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Histopathology 2nd Revised edition [Mīkstie vāki]

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Edited by (Editor, ^IBritish Journal of Biomedical Science^R), Edited by (Consultant Grade Biomedical Scientist/Laboratory Manager, Viapath, St. John's Institute of Dermatology, London)
  • Formāts: Paperback / softback, 520 pages, height x width x depth: 247x190x21 mm, weight: 992 g, 375 colour illustrations
  • Sērija : Fundamentals of Biomedical Science
  • Izdošanas datums: 16-Nov-2017
  • Izdevniecība: Oxford University Press
  • ISBN-10: 0198717334
  • ISBN-13: 9780198717331
  • Mīkstie vāki
  • Cena: 71,61 €
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  • Formāts: Paperback / softback, 520 pages, height x width x depth: 247x190x21 mm, weight: 992 g, 375 colour illustrations
  • Sērija : Fundamentals of Biomedical Science
  • Izdošanas datums: 16-Nov-2017
  • Izdevniecība: Oxford University Press
  • ISBN-10: 0198717334
  • ISBN-13: 9780198717331
Histopathology describes the processes and practices that are central to the role of the histopathologist within a functioning diagnostic laboratory, from pre-sampling to diagnosis to laboratory management.

Biomedical scientists are the foundation of modern healthcare, from cancer screening to diagnosing HIV, from blood transfusion for surgery to food poisoning and infection control. Without biomedical scientists the diagnosis of disease, the evaluation of the effectiveness of treatment, and research into the causes and cures of disease would not be possible.

The Fundamentals of Biomedical Science series has been written to reflect the challenges of practicing biomedical science today. It draws together essential basic science with insights into laboratory practice to show how an understanding of the biology of disease is coupled to the analytical approaches that lead to diagnosis. Assuming only a minimum of prior knowledge, the series reviews the full range of disciplines to which a Biomedical Scientist may be exposedfrom microbiology to cytopathology to transfusion science.

Histopathology describes the processes and practices that are central to the role of the histopathology biomedical scientist, from presampling to diagnosis to laboratory management. It demonstrates throughout how an understanding of cell and tissue physiology is vital to the successful identification of clinical conditions.

Recenzijas

It is very simply and clearly written in language that is easy to understand for the non-specialist. It describes day-to-day histopathology lab practice with clarity and the case studies are very helpful to illustrate the applications of the techniques. * Susan Brooks, Oxford Brookes University * The strength of the book is its detail. The book contains information that is completely lacking from other histopathology books. Overall I think that it is a great textbook for biomedical students and academics. * Akis Karakesisoglou, Durham University * Any potential biomedical scientist with an interest in histopathology, and sound knowledge of the contents of this well- written and nicely-illustrated text together with appropriate practical experience would be well on their way to becoming a valuable member of the team that comprises the core of todays histopathology laboratory. The text will make a valuable addition to any library associated with laboratories performing histopathological techniques. * Judy Brincat, Histology Group of Victoria *

Foreword vii
An introduction to the Fundamentals of Biomedical Science series viii
Contributors xii
1 What is histopathology? 1(26)
David Muskett
Guy Orchard
Anne Warren
1.1 Introduction
1(1)
1.2 What is pathology?
2(1)
1.3 What is histopathology?
3(1)
1.4 Histopathology specimens
4(1)
1.5 Histopathology service users
5(1)
1.6 Patients
6(1)
1.7 Post-mortem specimens
7(1)
1.8 Human Tissue Authority
8(1)
1.9 Clinical trials
8(1)
1.10 Laboratory processing
9(4)
1.11 Reporting results
13(2)
1.12 Reporting
15(3)
1.13 Health and safety
18(3)
1.14 Public health considerations
21(1)
1.15 Cancer registration
22(1)
1.16 Laboratory information management systems
22(1)
1.17 Report production and distribution
23(1)
1.18 Communication with clinicians
23(1)
1.19 Archiving
23(1)
1.20 Quality assurance
23(2)
Chapter Summary
25(1)
Further Reading
25(1)
Discussion Questions
25(2)
2 Fixation and specimen handling 27(23)
David Muskett
2.1 Introduction
27(1)
2.2 Specimen collection
28(2)
2.3 Specimen reception
30(4)
2.4 Cell and tissue preservation
34(6)
2.5 Key fixatives
40(5)
2.6 Decalcification
45(3)
Chapter Summary
48(1)
Further Reading
49(1)
Discussion Questions
49(1)
3 Data recording and histopathological dissection 50(43)
Vanda McTaggert
Sue Pritchard
Anne Warren
3.1 Pre-analysis
51(3)
3.2 Specimen types
54(2)
3.3 Fundamentals of dissection
56(4)
3.4 Organ systems
60(22)
3.5 Head and neck pathology
82(3)
3.6 Gynaecological cases
85(5)
3.7 Storage and retention
90(1)
Chapter Summary
91(1)
Further Reading
92(1)
Discussion Questions
92(1)
4 Routine processing, embedding and staining 93(24)
David Muskett
4.1 Introduction
93(1)
4.2 Tissue processing
94(2)
4.3 Tissue processing in practice
96(6)
4.4 Embedding
102(2)
4.5 Microtomy
104(4)
4.6 Cryotechniques and cryotomy
108(3)
4.7 Haematoxylin and eosin staining
111(1)
4.8 Staining equipment
112(1)
4.9 Coverslips
113(1)
4.10 Quality assurance
114(1)
Chapter Summary
115(1)
Further Reading
116(1)
Discussion Questions
116(1)
5 Stains in action 117(52)
David Muskett
Guy Orchard
5.1 Introduction
117(1)
5.2 Underlying principles of staining
118(2)
5.3 Differential action of dyes
120(2)
5.4 Special staining in practice
122(4)
5.5 Carbohydrates, mucins and glycoprotein demonstration
126(10)
5.6 Infective agent demonstration
136(9)
5.7 Extracellular proteins and connective tissues
145(6)
5.8 Lipids
151(2)
5.9 Pigments and minerals
153(7)
5.10 Liver biopsy
160(2)
5.11 Native renal biopsy
162(1)
5.12 Muscle biopsy
162(3)
5.13 Nerve biopsy
165(1)
Chapter Summary
166(1)
Further Reading
167(1)
Discussion Questions
168(1)
6 Artefacts 169(28)
Guy Orchard
Chantell Hodgson
Brian Nation
6.1 Introduction
169(1)
6.2 Concepts and classification criteria
170(1)
6.3 Pre-analytical artefacts
171(5)
6.4 Post-analytical artefacts
176(19)
Chapter Summary
195(1)
Further Reading
196(1)
Discussion Questions
196(1)
7 Mohs procedures 197(21)
Guy Orchard
Mohammad Shams
7.1 Introduction
197(1)
7.2 Historical perspective
198(1)
7.3 Principle of Mohs micrographic surgery
199(1)
7.4 Mohs application in the removal of skin and mucosal tumours
199(2)
7.5 The Mohs laboratory set up and key equipment
201(3)
7.6 Tissue inking, mapping and cut-up
204(2)
7.7 Tissue embedding and orientation procedures
206(1)
7.8 Tissue cryotomy
207(2)
7.9 Tissue section slide mounting and staining
209(2)
7.10 Troubleshooting
211(1)
7.11 Basic microscope interpretation
212(1)
7.12 Slow Mohs
212(2)
7.13 Immunocytochemistry and Mohs
214(1)
7.14 Health and safety, and professional standards
215(1)
7.15 Future developments
215(1)
Chapter Summary
216(1)
Further Reading
217(1)
Discussion Questions
217(1)
8 Immunocytochemical techniques 218(33)
Merdol Ibrahim
Guy Orchard
8.1 Introduction
218(1)
8.2 Historical perspective
219(1)
8.3 Immunocytochemistry in diagnostic histopathology
220(1)
8.4 Tissue preparation
221(4)
8.5 Antigen retrieval methods
225(7)
8.6 Immunocytochemistry detection methods
232(5)
8.7 Automation in immunocytochemistry
237(1)
8.8 Quality control and immunocytochemistry
237(7)
8.9 Health and safety in the ICC laboratory
244(1)
8.10 The value of ICC in multidisciplinary team meetings
245(2)
8.11 Future developments in immunocytochemistry
247(1)
Chapter Summary
248(1)
Further Reading
249(1)
Discussion Questions
250(1)
9 Analytical immunocytochemistry 251(47)
Guy Orchard
David Muskett
Anne Warren
9.1 Introduction
251(1)
9.2 Key principles in the diagnostic use of immunocytochemistry
252(1)
9.3 Reasons for immunocytochemical investigations
253(1)
9.4 What is malignancy?
253(1)
9.5 Introduction to tumour groups
254(1)
9.6 Key antibodies used in immunocytochemistry
255(2)
9.7 Use of antibody panels
257(1)
9.8 Antibody panels in diagnostic practice
258(1)
9.9 Investigation of breast cancer
259(5)
9.10 Investigation of lung cancer
264(5)
9.11 Investigation of prostate cancer
269(4)
9.12 Distinguishing lymphoma types
273(4)
9.13 Assessment of sentinel lymph nodes
277(2)
9.14 Identification of aetiological agents
279(1)
9.15 Identifying autoimmune states
280(1)
9.16 Algorithmic approach to the use of antibody panels
281(1)
9.17 Identification of tumours of unknown aetiology
282(3)
9.18 Immunocytochemistry as an indicator of suitability for therapy
285(4)
Chapter Summary
289(7)
Further Reading
296(1)
Discussion Questions
297(1)
10 In situ hybridization: key concepts and applications 298(20)
Anthony Warford
Emanuela Volpi
10.1 Introduction
298(2)
10.2 Technology
300(8)
10.3 Applications for ISH in diagnostic cellular pathology
308(4)
10.4 Future directions
312(1)
Chapter Summary
313(1)
Further Reading
314(3)
Discussion Questions
317(1)
11 Molecular diagnostics: techniques and applications 318(27)
Brendan O'Sullivan
Philippe Taniere
11.1 Introduction
318(2)
11.2 Essential background knowledge
320(4)
11.3 The polymerase chain reaction
324(4)
11.4 Laboratory workflow
328(4)
11.5 Processing the sample
332(1)
11.6 Pre-extraction sample handling and assessment
333(2)
11.7 Nucleic acid extraction
335(3)
11.8 PCR analysis methods
338(3)
Chapter Summary
341(1)
Further Reading
342(2)
Discussion Questions
344(1)
12 Molecular diagnostics in action 345(20)
Philippe Taniere
12.1 Introduction
345(3)
12.2 Molecular pathology diagnostic services: the mission
348(1)
12.3 Mutation testing
349(3)
12.4 Choosing the appropriate technique
352(1)
12.5 Multiplex gene testing
353(1)
12.6 Protein assessment
354(1)
12.7 Automation of protein assessment
355(1)
12.8 Predictive markers to non-targeted therapy
355(1)
12.9 Reporting
356(1)
12.10 Circulating free tumour DNA
356(1)
12.11 Accreditation
357(1)
12.12 Molecular pathology in perspective
357(1)
12.13 Case studies
358(5)
Chapter Summary
363(1)
Further Reading
363(1)
Discussion Questions
364(1)
13 Histopathology reporting 365(22)
Guy Orchard
Chris Ward
Susan Pritchard
13.1 What is a histopathology report?
365(2)
13.2 The microscopy content of a histopathology report
367(1)
13.3 Reporting datasets and tissue pathways
368(1)
13.4 Inflammation
369(6)
13.5 Neoplasia
375(6)
13.6 Research and development in histopathology: how does it improve diagnosis?
381(1)
13.7 Extended roles for biomedical and clinical scientists in histopathology
381(2)
13.8 Histopathology scientific qualifications in Australia and New Zealand
383(2)
Chapter Summary
385(1)
Further Reading
385(1)
Discussion Questions
386(1)
14 Light microscopy and digital pathology 387(22)
Guy Orchard
14.1 Introduction
387(1)
14.2 Scientific principles behind light microscopy
388(2)
14.3 Image formation and lens defects
390(1)
14.4 Components of the compound microscope
391(3)
14.5 Contrast techniques
394(1)
14.6 Phase contrast microscope
395(2)
14.7 Polarization microscope
397(1)
14.8 Darkfield microscope
398(1)
14.9 Fluorescence microscope
399(3)
14.10 Confocal microscopy and optical sectioning
402(1)
14.11 Alternative microscope designs
403(1)
14.12 Sharing the microscope image
404(3)
14.13 The future
407(1)
Chapter Summary
408(1)
Further Reading
408(1)
Discussion Questions
408(1)
15 Electron microscopy 409(32)
David N. Furness
15.1 Introduction
409(1)
15.2 General introduction to electron microscopy
410(2)
15.3 Design of transmission electron microscopes
412(5)
15.4 Design of scanning electron microscopes
417(2)
15.5 Specific tissue preparation for TEM
419(9)
15.6 Preparation for SEM
428(1)
15.7 Case Studies/Applications
429(9)
15.8 Other applications
438(1)
Chapter Summary
439(1)
Further Reading
439(1)
Discussion Questions
440(1)
16 Mortuary practice 441(22)
lshbel Gall
16.1 Introduction
441(1)
16.2 The mortuary
441(3)
16.3 Death and decomposition
444(1)
16.4 Certification
445(2)
16.5 Other documentation
447(1)
16.6 Post-mortem examinations
448(11)
16.7 Reconstruction
459(1)
16.8 Disinfection
459(1)
Chapter Summary
460(1)
Further Reading
461(1)
Discussion Questions
462(1)
17 Essentials of laboratory management 463(22)
Sue Alexander
Patricia Fernando
17.1 Introduction
463(2)
17.2 Roles in laboratory management
465(3)
17.3 Quality management systems and clinical governance
468(5)
17.4 Clinical governance
473(2)
17.5 Risk management
475(3)
17.6 Dealing with complaints
478(1)
17.7 Human Tissue Act
478(3)
17.8 Training and qualifications
481(1)
Chapter Summary
482(1)
Further Reading
482(1)
Discussion Questions
483(2)
Glossary 485(3)
Abbreviations 488(3)
Index 491
Dr Guy Orchard is Consultant Grade Biomedical Scientist, Laboratory Manager at Viapath, St John's Institute of Dermatology, and Viapath Operational Lead for Head and Neck Pathology at Guys Hospital, London.

Dr Brian Nation is Editor of The Biomedical Scientist, a publication of the Institute of Biomedical Science.