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Histopathology [Mīkstie vāki]

3.82/5 (21 ratings by Goodreads)
Edited by (ViaPath (formerly GSTS Pathology), London), Edited by (Editor, British Journal of Biomedical Science)
  • Formāts: Paperback / softback, 416 pages, height x width x depth: 247x190x18 mm, weight: 891 g, 77 line illustrations and 33 half-tone
  • Sērija : Fundamentals of Biomedical Science
  • Izdošanas datums: 29-Sep-2011
  • Izdevniecība: Oxford University Press
  • ISBN-10: 0199574340
  • ISBN-13: 9780199574346
  • Mīkstie vāki
  • Cena: 68,38 €*
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  • Formāts: Paperback / softback, 416 pages, height x width x depth: 247x190x18 mm, weight: 891 g, 77 line illustrations and 33 half-tone
  • Sērija : Fundamentals of Biomedical Science
  • Izdošanas datums: 29-Sep-2011
  • Izdevniecība: Oxford University Press
  • ISBN-10: 0199574340
  • ISBN-13: 9780199574346
Biomedical scientists are the foundation of modern healthcare, from cancer screening to diagnosing HIV, from blood transfusion for surgery to food poisoning and infection control. Without biomedical scientists, the diagnosis of disease, the evaluation of the effectiveness of treatment, and research into the causes and cures of disease would not be possible. The Fundamentals of Biomedical Science series is written to reflect the challenges of practicing biomedical science today. It draws together essential basic science with insights into laboratory practice to show how an understanding of the biology of disease is coupled to the analytical approaches that lead to diagnosis. Assuming only a minimum of prior knowledge, the series reviews the full range of disciplines to which a Biomedical Scientist may be exposed - from microbiology to cytopathology to transfusion science. Alongside volumes exploring specific biomedical themes and related laboratory diagnosis, an overarching Biomedical Science Practice volume gives a grounding in the professional and experimental skills with which any Biomedical Scientist must be equipped.

The series · Understands the complex roles of Biomedical Scientists in the modern practice of medicine · Understands the development needs of employers and the Profession · Addresses the need for understanding of a range of fundamental sciences in the context of Biomedicine · Places the theoretical aspects of Biomedical Science in their practical context

The series is developed in partnership with and endorsed by the Institute of Biomedical Science. See ibms.org for more information.

Histopathology describes the processes and practices that are central to the role of the histopathology biomedical scientist, from pre-sampling to diagnosis to laboratory management.

An understanding of the physiology of cells and tissue is vital to the successful identification of clinical conditions and, as such, this book begins by laying out the key features of both and their significance for diagnosis. But histopathology relies heavily on optical technology, and microscopy is covered in detail from fixation, through staining to light and electron imaging methods.

A complete course on the concepts and skills needed for a biomedical scientist to work within a functioning histopathology laboratory.

Online Resource Centre The Online Resource Centre to accompany Histopathology features:

For registered adopters of the book: · Figures from the book, available to download

For students: · An interactive Digital Microscope, encouraging the exploration of tissue samples · Self-assessment modules to help you to check and reinforce understanding of the basic science introduced in each volume · Video podcasts including interviews with practicing biomedical scientists, and 'in the lab' footage showing biomedical science in practice

Recenzijas

"...well-written, systematically laid-out and nicely-illustrated text... will make a valuable addition to any library associated with laboratories performing histopathological techniques." * Judy Brincat, Histology Group of Victoria * The book is well illustrated with numerous high quality photomicrographs and well thought out diagrams. Case studies on classic lesions encountered in everyday practice are especially illustrative for the practice of histopathology... an excellent textbook for anybody interested in pursuing a career involving histopathology. * Journal of Histotechnology, July 2012 * All bioscience students should have access to such a textbook because of its intricate layout, clearly annotated pictures and its ability to cut straight to the important parts of histopathology. * Dan Bell, student, Liverpool Hope University *

1 What is histopathology? 1(33)
David Evans
Max Robinson
1.1 Introduction
1(5)
1.2 Key criteria in the production of a histopathology report
6(1)
1.3 Types of biopsy
7(1)
1.4 Tissue fixation
7(3)
1.5 Specimen collection, transport and receipt in the laboratory
10(2)
1.6 Macroscopic examination, trimming and block selection
12(1)
1.7 Tissue processing
12(2)
1.8 Tissue embedding
14(1)
1.9 Microtomy
15(3)
1.10 Staining and mounting
18(1)
1.11 Reporting
19(7)
1.12 Quality assurance
26(3)
1.13 Case studies
29(3)
Chapter summary
32(1)
Further reading
32(1)
Discussion questions
33(1)
2 Staining-principles and demonstration techniques 34(45)
Harry Elliott
2.1 Introduction
34(1)
2.2 Principles of staining
35(1)
2.3 Aims of staining
35(1)
2.4 Fixation effects
35(1)
2.5 Types of staining
36(2)
2.6 Stains
38(4)
2.7 Mechanisms of staining
42(7)
2.8 Some staining methods used in histopathology
49(27)
2.9 Applications of tinctorial and histochemical staining
76(1)
Chapter summary
77(1)
Further reading
77(1)
Discussion questions
77(2)
3 From specimen to slide 79(48)
David Muskett
3.1 Introduction
79(2)
3.2 Definitions
81(2)
3.3 Sending samples to histopathology
83(4)
3.4 Cell and tissue preservation
87(9)
3.5 Specimen handling
96(7)
3.6 Tissue processing and embedding
103(7)
3.7 Embedding
110(2)
3.8 Sectioning
112(8)
3.9 Staining, coverslipping and quality
120(5)
Chapter summary
125(1)
Further reading
125(1)
Discussion questions
126(1)
4 Stains in action 127(43)
David Muskett
4.1 Introduction
127(1)
4.2 Nomenclature
128(1)
4.3 Stains and dyes
128(1)
4.4 Special stains in practice
129(1)
4.5 Special stains in the laboratory
129(3)
4.6 Carbohydrate staining
132(9)
4.7 Identifying microorganisms
141(8)
4.8 Identifying pigments and minerals
149(6)
4.9 Extracellular proteins
155(7)
4.10 Lipids
162(2)
4.11 Liver biopsy-special stains
164(4)
Chapter summary
168(1)
Further reading
168(1)
Discussion questions
169(1)
5 Immunocytochemistry in diagnostic histopathology 170(34)
Guy Orchard
Alex Javed
5.1 Introduction
170(1)
5.2 Historical perspective
171(1)
5.3 Immunocytochemistry in diagnostic histopathology
172(1)
5.4 Tissue preparation
173(4)
5.5 Antigen retrieval methods
177(8)
5.6 Immunocytochemistry detection methods
185(5)
5.7 Quality control and ICC
190(8)
5.8 Automation in ICC
198(1)
5.9 Health and safety in the ICC laboratory
199(1)
5.10 The value of ICC in multidisciplinary team meetings
199(1)
5.11 Future developments in ICC
200(1)
Chapter summary
201(1)
Further reading
201(1)
Discussion questions
202(2)
6 Immunocytochemistry in action 204(48)
David Muskett
Guy Orchard
6.1 Introduction
204(2)
6.2 Key principles in the diagnostic use of immunocytochemistry
206(1)
6.3 Reasons for immunocytochemical investigations
206(1)
6.4 What is malignancy?
206(2)
6.5 Introduction to tumour groups
208(1)
6.6 Key antibodies used in immunocytochemistry
209(10)
6.7 Use of antibody panels
219(1)
6.8 Antibody panels in diagnostic practice
220(2)
6.9 Investigation of breast cancer
222(5)
6.10 Investigation of lung cancer
227(4)
6.11 Investigation of prostate cancer
231(4)
6.12 Distinguishing lymphoma types
235(5)
6.13 Assessment of sentinel lymph nodes
240(2)
6.14 Identification of aetiological agents
242(1)
6.15 Identifying autoimmune states
243(2)
6.16 Algorithmic approach to the use of antibody panels
245(1)
6.17 Identification of tumours of unknown aetiology
246(2)
6.18 Immunocytochemistry as an indicator of suitability for therapy
248(1)
Chapter summary
249(1)
Further reading
250(1)
Discussion questions
250(2)
7 Molecular diagnostics in histopathology 252(34)
Diana Cullen
Lisa Wheatley
Guy Orchard
7.1 Introduction
252(1)
7.2 Essential background knowledge
253(5)
7.3 Probe preparation and labelling
258(1)
7.4 In situ hybridization
259(5)
7.5 Southern blotting
264(4)
7.6 Polymerase chain reaction
268(7)
7.7 Application of molecular techniques
275(6)
7.8 Quality
281(1)
7.9 Current developments and a look to the future
282(2)
7.10 Molecular diagnostics in the real world
284(1)
Chapter summary
284(1)
Further reading
285(1)
Discussion questions
285(1)
8 Light Microscopy 286(24)
David Crook
Tony Sims
8.1 Introduction
286(1)
8.2 Scientific principles behind light microscopy
287(2)
8.3 Image formation and lens defects
289(1)
8.4 Components of the compound microscope
290(5)
8.5 Contrast techniques
295(1)
8.6 Phase contrast microscope
296(1)
8.7 Polarization microscope
297(1)
8.8 Darkfield microscope
298(1)
8.9 Fluorescence microscope
299(4)
8.10 Confocal microscopy and optical sectioning
303(1)
8.11 Alternative microscope designs
304(1)
8.12 Sharing the microscope image
305(3)
8.13 The future
308(1)
Chapter summary
309(1)
Further reading
309(1)
Discussion questions
309(1)
9 Transmission electron microscopy 310(35)
Tracey de Haro
9.1 Introduction
310(1)
9.2 General introduction to transmission electron microscopy
311(1)
9.3 Design of the electron microscope
312(7)
9.4 Specific tissue preparation for electron microscopy
319(9)
9.5 Case studies/applications
328(13)
9.6 Other applications
341(1)
Chapter summary
342(1)
Further reading
343(1)
Discussion questions
344(1)
10 Essentials of laboratory management 345(16)
Barbara Lloyd
10.1 Introduction
345(1)
10.2 Roles in laboratory management
346(1)
10.3 Quality management systems
347(4)
10.4 Clinical governance
351(1)
10.5 Risk management
352(3)
10.6 Dealing with complaints
355(1)
10.7 Human Tissue Act
356(3)
Chapter summary
359(1)
Further reading
359(1)
Discussion questions
360(1)
11 Mortuary practice 361(22)
Ishbel Gall
11.1 Introduction
361(1)
11.2 The mortuary
361(3)
11.3 Death and decomposition
364(1)
11.4 Certification
365(1)
11.5 Other documentation
366(2)
11.6 Post-mortem examinations
368(11)
11.7 Reconstruction
379(1)
11.8 Disinfection
379(1)
Chapter summary
380(1)
Further information
381(1)
Discussion questions
382(1)
Glossary 383(3)
Abbreviations 386(2)
Index 388
Dr Guy Orchard is Consultant Grade Biomedical Scientist, Laboratory Manager at Viapath, St John's Institute of Dermatology, and Viapath Operational Lead for Head and Neck Pathology at Guys Hospital, London.

Step Publishing, Institute of Biomedical Science, UK. Brian Nation is the editor of The Biomedical Scientist and British Journal of Biomedical Science.