Immuno-Oncology And Immunotherapy Part A Volume 189, in the Methods in Cell Biology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. Specific chapters to this release include, Generation of transmitochondrial cybrids in cancer cells, Methods for flow cytometry analysis for tumor infiltrating cells functions and phenotype, Innovative 2D and 3D methods of tumor cells culture, and more.
1. Assessment of translation rate in leukemic cells and immune cells of
the microenvironment by OPP protein synthesis assay
Vanessa Klapp, Ozgu Gumustekin, Jérōme Paggetti, Etienne Moussay and Anne
Largeot
2. Generation of transmitochondrial cybrids in cancer cells
Ruth Soler-Agesta, Cristina Ripollés-Yuba, Joaquķn Marco-Brualla, Raquel
Moreno-Loshuertos, Ai Sato, Manuel Beltrįn-Visiedo, Lorenzo Galluzzi and
Alberto Anel
3. Enzyme-linked Immunospot (Elispot) assay to quantify peptide-specific
IFN- production by splenocytes in a mouse tumor model after radiation
therapy
Benoīt Lecoester, Yaoyao Xie, Amélie Marguier, Laura Boulerot, Marine
Malfroy, Olivier Adotévi and Jihane Boustani
4. Tumor slice culture system for ex vivo immunotherapy studies
Leire Arrizabalaga, Joan Salvador Russo-Cabrera, Virginia Belsśe, Pedro
Berraondo, Ignacio Melero and Fernando Aranda
5. Identification and analysis of alloreactive T lymphocytes from peripheral
blood mononuclear cells
Alberto Susana, Giovanni Galletti, Gabriele De Simone, Chiara Camisaschi and
Enrico Lugli
6. Assessment of pDCs functional capacity upon exposure to tumor-derived
soluble factors
Vladimķr Kouckż, Linn A. Syding, Klįra Plakovį, Lucie Pavelkovį and Anna
Fialovį
7. Degranulation assay to evaluate NK cell natural and antibody-dependent
cell-mediated cytotoxicity against A549 tumor spheroids
Ainara Lopez-Pardo, Ainhoa Amarilla-Irusta, Vķctor Sandį, Mario Stan,
Francisco Borrego and Laura Amo
8. Characterization of Natural Killer cells infiltrating irradiated murine
tumors through flow cytometry
Jérémy Baude, Riad Ladjohounlou, Emeric Limagne, Lisa Froidurot, Véronique
Morgand and Céline Mirjolet
9. Flow cytometry detection and quantification of circulating leukocyte
subpopulations in mice after brain irradiation
Julie Coupey, Marine M. Leblond, Erika S. Hue and Samuel Valable
10. Flow cytometry-assisted analysis of phenotypic maturation markers on an
immortalized dendritic cell line
Ginevra Campia, Manuel Beltrįn-Visiedo, Ruth Soler-Agesta, Ai Sato, Norma
Bloy, Liwei Zhao, Peng Liu, Oliver Kepp, Guido Kroemer, Lorenzo Galluzzi and
Claudia Galassi
11. Optimization and intra-assay validation of a multiparametric flow
cytometric test for monitoring circulating TREGs
Iole Macchia, Floriana Iacobone and Francesca Urbani
12. Cytofluorometric assessment of calreticulin exposure on CD38+ plasma
cells from the human bone marrow
Manuel Beltrįn-Visiedo, Alfonso Serrano-Del Valle, Nelia Jiménez-Aldśan, Ruth
Soler-Agesta, Javier Naval, Lorenzo Galluzzi and Isabel Marzo
Lorenzo Galluzzi is Assistant Professor of Cell Biology in Radiation Oncology at the Department of Radiation Oncology of the Weill Cornell Medical College, Honorary Assistant Professor Adjunct with the Department of Dermatology of the Yale School of Medicine, Honorary Associate Professor with the Faculty of Medicine of the University of Paris, and Faculty Member with the Graduate School of Biomedical Sciences and Biotechnology of the University of Ferrara, the Graduate School of Pharmacological Sciences of the University of Padova, and the Graduate School of Network Oncology and Precision Medicine of the University of Rome La Sapienza. Moreover, he is Associate Director of the European Academy for Tumor Immunology and Founding Member of the European Research Institute for Integrated Cellular Pathology.
Galluzzi is best known for major experimental and conceptual contributions to the fields of cell death, autophagy, tumor metabolism and tumor immunology. He has published over 450 articles in international peer-reviewed journals and is the Editor-in-Chief of four journals: OncoImmunology (which he co-founded in 2011), International Review of Cell and Molecular Biology, Methods in Cell biology, and Molecular and Cellular Oncology (which he co-founded in 2013). Additionally, he serves as Founding Editor for Microbial Cell and Cell Stress, and Associate Editor for Cell Death and Disease, Pharmacological Research and iScience. Norma received her masters degree in health biology from Paris Saclay. In 2013, she joined the laboratory of Dr. Guido Kroemer at the Centre de Recherche des Cordeliers (Paris, France) and at Gustave Roussy (Villejuif, France), the largest center for oncological patients in Europe. She worked on several projects linked to immunosurveillance, culminating with her obtaining her PhD in 2017 with an original work on Immunogenic stress and death of cancer cells: Contribution of antigenicity vs adjuvanticity to immunosurveillance. She then moved to Weill Cornell Medicine to join the program in radiation and immunity under the mentorship of Dr. Galluzzi. Her current research is focused on investigating resistance to immunotherapy in a mouse model of HR+ breast cancer.
Maud Charpentier received her M.Sc. in Cellular Biology and her Ph.D. in Immunology from the University of Nantes, France. She chose to pursue an academic career and continued her postdoctoral training in the United States. She joined the Department of Radiation Oncology at Weill Cornell Medicine under the mentorship of Dr. Sandra Demaria. Maud has a long-standing interest in the anti-tumor immune response and its role in controlling cancer progression and treatment outcomes. Her research focuses on understanding the synergy between radiation therapy and immunotherapy in solid tumors, with the aim of overcoming resistance to treatment and developing innovative therapeutic approaches in preclinical models.
