Neuroscientists, psychiatrists, pathologists, oncologists, surgeons, microbiologists, and other researchers and physicians from around the world present 36 contributions which address the major techniques in interleukin research methodologies currently available. The five sections address methods for assaying interleukin protein and mRNA, including the use of ELISA; the assays of interleukins in specific pathologies; the assaying of interleukins in different biological fluids relevant to disease; and newer methods in the study of interleukin signal transduction, analysis of polymorphisms in interleukin genes, and the use of cDNA arrays. Of likely interest to a wide array of scientific investigators including molecular and cell biologists and immunologists. Annotation c. Book News, Inc., Portland, OR (booknews.com)
Luke O'Neill and Andrew Bowie bring together a collection of standard and advanced methods for measuring these powerful agents. Their readily reproducible techniques range from the assay of interleukin protein and mRNA-using ELISA, FACS, and RT-PCR-to the study of interleukin signal transduction. Newer techniques are also covered, including the analysis of interleukin gene polymorphisms and the use of cDNA microarrays. Many of the assays are geared to specific pathologies, including breast cancer, depression, psoriasis, Grave's disease, migraine, and myocardial infarction. Comprehensive and highly practical, Interleukin Protocols offers biomedical investigators a stellar collection of all the major techniques needed to analyze the role of interleukins in disease, to improve diagnosis, and to foster the rapid emergence of new and more powerful therapeutics.
