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E-grāmata: Intracellular Pathogens: Methods and Protocols

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  • Formāts: EPUB+DRM
  • Sērija : Methods in Molecular Biology 2813
  • Izdošanas datums: 18-Jun-2024
  • Izdevniecība: Springer-Verlag New York Inc.
  • Valoda: eng
  • ISBN-13: 9781071638903
  • Formāts - EPUB+DRM
  • Cena: 237,93 €*
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  • Formāts: EPUB+DRM
  • Sērija : Methods in Molecular Biology 2813
  • Izdošanas datums: 18-Jun-2024
  • Izdevniecība: Springer-Verlag New York Inc.
  • Valoda: eng
  • ISBN-13: 9781071638903

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This detailed volume presents cutting-edge approaches to study infection, immunity, and intervention strategies against intracellular pathogens. The book explores areas such as how intracellular pathogens infect and survive inside the phagocytes, immunopathological features of infection, evasion of host-induced immune responses, cellular crosstalk in the presence of intracellular pathogens, host innate and adaptive immunity, and much more. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Intracellular Pathogens: Methods and Protocols serves as an ideal guide for researchers who work with intracellular pathogens and want to utilize or refine strategies to study, treat, or controlthem.
Intracellular Pathogens: Infection, Immunity, and Intervention.- Methods
for Genomic Epidemiology of Bacterial Pathogens: Example Salmonella.-
Phagocytosis.- Large Scale Culture and Plasmid Preparation Procedure for
Low-Yield Bacmids Containing Full-Length SARS-CoV-2 cDNAs.-
Metatranscriptomic RNA-Seq Data Analysis of Virus-Infected Host Cells.-
Metabolic Analysis of Intracellular Pathogenic Bacteria Using NMR.-
Quantitative Proteomics of the Intracellular Bacterial Pathogen Salmonella
enterica Serovar Typhimurium.- Median Tissue Culture Infectious Dose 50
(TCID50) Assay to Determine Infectivity of Cytopathic Viruses.- Applications
of Quantitative PCR (qPCR) in Studies of Virus-Host Interactions.-
Establishing Air-Liquid Interface (ALI) Airway Culture Models for Infectious
Disease Research.- Pig Acute Phase Proteins as Non-Antibody Systemic
Biomarkers of Intracellular Infections.- Quantification of Mycobacterium
tuberculosis Growth in Cell-Based Infection Assays by Time-Lapse Fluorescence
Microscopy.- Intravital Microscopy for Imaging and Live Cell Tracking of
Alveolar Macrophages in Real Time.- Recent Advancements in
Radiopharmaceuticals for Infection Imaging.- Bacteriophages Isolation and
Efficacy Testing.- Production, Isolation, and Detection of Poly IC in
Extracellular Vesicles from U937 Cells.- In Silico Tools for Predicting Novel
Epitopes.- In Situ Characterization of Human Follicular Helper CD4 T Cells.-
Identification and Characterization of Antigen-Specific T-Cells in Viral
Infections.- Evaluating Cationic Nanoparticles as Carriers of Antiviral
Nucleic Acids.- RNA-Based Vaccines and Therapeutics Against Intracellular
Pathogens.