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E-grāmata: Jasmonate Signaling: Methods and Protocols

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  • Formāts: EPUB+DRM
  • Sērija : Methods in Molecular Biology 1011
  • Izdošanas datums: 08-Jul-2014
  • Izdevniecība: Humana Press Inc.
  • Valoda: eng
  • ISBN-13: 9781627034142
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  • Formāts: EPUB+DRM
  • Sērija : Methods in Molecular Biology 1011
  • Izdošanas datums: 08-Jul-2014
  • Izdevniecība: Humana Press Inc.
  • Valoda: eng
  • ISBN-13: 9781627034142
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It is now well established that jasmonates, originally identified as the major component of jasmine scent, play a universal role in the plant kingdom and are involved in the regulation of diverse aspects of plant biology, including growth, development, metabolism, and interaction with the environment. In Jasmonate Signaling: Methods and Protocols, experts in the field aim to unite powerful emerging omics platforms with a number of key reductionist approaches to form a comprehensive collection of tools and protocols. The detailed chapters in this book embrace physiological, environmental, molecular, omics, and bioinformatics approaches that allow dissecting jasmonate actions in the model species Arabidopsis thaliana or in other plants. Written in the highly successful Methods in Molecular Biology series format, chapters feature introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, along with tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Jasmonate Signaling: Methods and Protocols will empower interested researchers to dissect all steps of jasmonate signaling and the processes they modulate.

This book examines physiological, environmental, molecular, omics, and bioinformatics approaches that allow dissecting jasmonate actions in the model species Arabidopsis thaliana or in other plants. It features readily reproducible laboratory protocols.
Phenotyping Jasmonate Regulation of Senescence.- Characterizing
Jasmonate Regulation of Male Fertility in Arabidopsis.- Phenotyping Jasmonate
Regulation of Root Growth.- Bioassays for Assessing Jasmonate-Dependent
Defenses Triggered by Pathogens, Herbivorous Insects, or Beneficial
Rhizobacteria.- Elicitation of Jasmonate-Mediated Defense Responses by
Mechanical Wounding and Insect Herbivory.- Pseudomonas syringae Infection
Assays in Arabidopsis.- Jasmonate Signaling in the Field, Part I: Elicited
Changes in Jasmonate Pools of Transgenic Nicotiana attenuata Populations.-
Jasmonate Signaling in the Field, Part II: Insect-Guided Characterization of
Genetic Variations in Jasmonate-Dependent Defenses of Transgenic and Natural
Nicotiana attenuata Populations.- Profiling of Jasmonic Acid-Related
Metabolites and Hormones in Wounded Leaves.- A Non-Targeted Approach for
Extended Liquid Chromatography-Mass Spectrometry Profiling of Free and
Esterified Jasmonates After Wounding.- Cell-Specific Detection of Jasmonates
by Means of an Immunocytological Approach.- Jasmonic Acid-Amino Acid
Conjugation Enzyme Assays.- Pull-Down Analysis of Interactions Among Jasmonic
Acid Core Signaling Proteins.- Yeast Two-Hybrid Analysis of Jasmonate
Signaling Proteins.- Modified Bimolecular Fluorescence Complementation Assay
to Study the Inhibition of Transcription Complex Formation by JAZ Proteins.-
Agroinfiltration of Nicotiana benthamiana Leaves for Co-Localization of
Regulatory Proteins Involved in Jasmonate Signaling.- Electrophoretic
Mobility Shift Assay for the Analysis of Interactions of Jasmonic
Acid-Responsive Transcription Factors with DNA.- Transient Expression Assays
in Tobacco Protoplasts.- Functional Analysis of Jasmonic Acid-Responsive
Secondary Metabolite Transporters.- Expression Analysis of
Jasmonate-Responsive Lectins in Plants.- Profiling the Jasmonic Acid
Responses by Nuclear Magnetic Resonance-Based Metabolomics.- Metabolite
Profiling of Plant Tissues by Liquid Chromatography Fourier Transform Ion
Cyclotron Resonance Mass Spectrometry.- cDNA-AFLP-Based Transcript Profiling
for Genome-Wide Expression Analysis of Jasmonate-Treated Plants and Plant
Cultures.- Analysis of RNA-Seq Data with TopHat and Cufflinks for Genome-Wide
Expression Analysis of Jasmonate-Treated Plants and Plant Cultures.-
Transcriptome Coexpression Analysis Using ATTED-II for Integrated
Transcriptomics/Metabolomics Analysis.- A Guide to CORNET for the
Construction of Coexpression and Protein-Protein Interaction Networks.