| Contributors |
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xv | |
| Fish as model systems |
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xix | |
| Section 1 Zebrafish (Danio rerio) |
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1 Biology and research applications |
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The biology of the zebrafish |
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3 | (3) |
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3 | (1) |
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Zebrafish appearance and life span |
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4 | (1) |
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Zebrafish phylogeny and genetics |
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4 | (1) |
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Zebrafish embryonic development |
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5 | (1) |
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The history of the zebrafish as laboratory model |
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6 | (2) |
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Resources for the zebrafish model |
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7 | (1) |
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The zebrafish as a model for development and pathology |
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8 | (9) |
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The zebrafish as model in developmental biology |
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8 | (1) |
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The zebrafish as model in pathology |
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8 | (1) |
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The zebrafish as a model for cancer research |
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9 | (2) |
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The zebrafish embryo in drug discovery and high-throughput screens |
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11 | (2) |
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The zebrafish as a model for toxicology |
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13 | (2) |
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The zebrafish as model for neurodegenerative diseases |
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15 | (1) |
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15 | (1) |
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Amyotrophic lateral sclerosis |
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16 | (1) |
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Lessons from the zebrafish model on muscle dystrophies |
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16 | (1) |
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17 | (6) |
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2 Housing and maintenance of zebrafish, new technologies in laboratory aquatic systems and considerations for facility design |
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Introduction to the model |
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23 | (6) |
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Defining your needs from an animal model and the impact on facility design |
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24 | (3) |
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27 | (2) |
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29 | (4) |
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29 | (1) |
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30 | (1) |
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Water disinfection and biological control |
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31 | (1) |
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32 | (1) |
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Establishing biofiltration |
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33 | (1) |
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34 | (1) |
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34 | (23) |
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Water parameter targets and dealing with anomalies |
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34 | (3) |
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37 | (1) |
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37 | (3) |
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40 | (6) |
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46 | (4) |
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50 | (5) |
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New and emerging technologies |
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55 | (2) |
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57 | (1) |
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58 | (4) |
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62 | (1) |
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3 Breeding and larviculture of zebrafish |
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63 | (1) |
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Should we mimic nature, or should we ignore it in laboratory breeding and husbandry of zebrafish: theoretical considerations from the perspective of experimental biology |
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64 | (1) |
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65 | (1) |
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Preparing zebrafish for breeding |
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66 | (2) |
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68 | (3) |
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Raising zebrafish juveniles is not as easy as often stated |
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71 | (1) |
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Hatching the eggs and caring for the larvae |
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71 | (2) |
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Caring for young juveniles |
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73 | (3) |
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Standardization of breeding and larviculture practices: the question of replicability and reproducibility |
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76 | (2) |
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78 | (3) |
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4 Health monitoring, disease, and clinical pathology |
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81 | (1) |
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Controlling and monitoring population health |
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82 | (1) |
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Husbandry, environmental conditions, and infection pressure |
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82 | (1) |
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82 | (1) |
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Daily recording of morbidity and mortality |
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83 | (1) |
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Pathogens and diseases to monitor |
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83 | (7) |
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84 | (2) |
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Pseudocapillaria tomentosa |
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86 | (1) |
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87 | (1) |
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88 | (2) |
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Fish and environmental samples |
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90 | (2) |
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90 | (1) |
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91 | (1) |
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91 | (1) |
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92 | (1) |
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Necropsy and fresh mounts |
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92 | (1) |
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92 | (1) |
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Polymerase chain reaction |
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93 | (1) |
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Determining the number of samples |
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93 | (1) |
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Defining an epidemiological unit |
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93 | (1) |
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Specific pathogen free for a threshold prevalence |
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94 | (1) |
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94 | (1) |
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Introduction of zebrafish colonies and biosecurity |
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94 | (3) |
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94 | (2) |
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96 | (1) |
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96 | (1) |
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97 | (1) |
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97 | (1) |
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98 | (3) |
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5 The welfare of zebrafish |
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101 | (1) |
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Measuring welfare in zebrafish |
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102 | (2) |
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Behavioral stress response |
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102 | (1) |
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Neuroendocrine stress response |
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103 | (1) |
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104 | (2) |
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104 | (2) |
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Strains, genetic modifications, and harmful phenotypes |
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106 | (1) |
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Refinements in regulated procedures |
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106 | (6) |
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Pain and analgesic considerations |
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107 | (1) |
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108 | (1) |
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108 | (1) |
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Welfare-focused systematic review of surgical zebrafish models for cardiac regeneration |
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109 | (1) |
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109 | (3) |
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Conclusion: the state of zebrafish welfare today |
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112 | (1) |
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113 | (1) |
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113 | (7) |
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6 Analgesia, anesthesia, and euthanasia in zebrafish |
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120 | (4) |
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121 | (2) |
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123 | (1) |
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124 | (1) |
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124 | (4) |
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126 | (1) |
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126 | (1) |
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127 | (1) |
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127 | (1) |
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128 | (1) |
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128 | (5) |
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131 | (1) |
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131 | (1) |
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132 | (1) |
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133 | (1) |
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133 | (6) |
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7 Transgenesis, mutagenesis, knockdown, and genetic colony management |
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139 | (1) |
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Ethical issues in genetic manipulation of laboratory fish |
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140 | (8) |
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Fish management during generation of mutants |
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140 | (3) |
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143 | (3) |
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Fish management during generation of transgenics |
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146 | (2) |
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Transgenic fish management |
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148 | (1) |
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Generation and management of morphants and CRISPants |
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148 | (2) |
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Management of CRISPants/morphants |
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150 | (1) |
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150 | (2) |
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152 | (1) |
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152 | (5) |
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8 Sperm cryopreservation, in vitro fertilization, and embryo freezing |
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157 | (6) |
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163 | (14) |
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Protocol for sperm cryopreservation and in vitro fertilization |
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163 | (9) |
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Protocol for embryo cryopreservation |
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172 | (5) |
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177 | (1) |
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177 | (1) |
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177 | (8) |
| Section 2 Other small freshwater fish |
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9 Medaka as a model teleost: characteristics and approaches of genetic modification |
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185 | (4) |
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185 | (3) |
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Husbandry and breeding under laboratory conditions |
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188 | (1) |
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Basic approaches of generation of transgenic/knockout medaka |
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189 | (4) |
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Conventional ways of introducing transgenes: plasmid/ BAC-based constructs-their merits and demerits |
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189 | (1) |
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Generation of knockout using TALEN/CRISPR |
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190 | (1) |
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Double promoter methods for the efficient screening of transgenic lines |
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191 | (2) |
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Introduction of new transgenic methods |
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193 | (15) |
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Transgenesis with DNA transposase |
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193 | (2) |
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phiC31 integrase-mediated transgenesis |
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195 | (5) |
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An advantage and a basic principle of CRISPR/Cas-based knock-in approaches in medaka |
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200 | (8) |
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208 | (1) |
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208 | (7) |
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10 Integrated analyses using medaka as a powerful model animal toward understanding various aspects of reproductive regulation |
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Medaka as a powerful model animal for research fields of reproduction |
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215 | (1) |
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Mechanisms of reproductive systems clarified by histological and physiological analyses |
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216 | (1) |
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Histological studies using medaka |
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217 | (6) |
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220 | (1) |
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221 | (1) |
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Single-cell labeling and tract tracing using axonal transport |
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222 | (1) |
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Conventional histological staining |
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222 | (1) |
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The present scheme of HPG axis regulation clarified by multidisciplinary analyses in vertebrates |
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223 | (3) |
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Differences between mammals and teleosts in terms of function of the kisspeptin neuronal system and HPG axis regulation |
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224 | (2) |
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Various novel functions of the kisspeptin neuronal system in teleosts |
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226 | (1) |
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Endocrine studies using medaka |
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226 | (4) |
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Surgery and blood sampling |
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227 | (1) |
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Hormonal analysis of sex steroid hormones |
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228 | (2) |
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Neurophysiological studies using medaka: patch clamp recording and calcium imaging |
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230 | (5) |
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Introduction to the neurophysiological analysis |
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230 | (1) |
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Neural activity analyzed by patch clamp recordings |
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231 | (2) |
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Hormone/neuropeptide release clarified by Cat} imaging |
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233 | (2) |
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Neurophysiology advanced the understanding of neuroendocrinological regulation of reproduction |
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235 | (1) |
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Medaka provides clues to the understanding of general mechanisms of reproduction in teleosts and even in vertebrates |
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235 | (2) |
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Understanding of mechanism of HPG axis regulation in medaka and teleosts |
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235 | (1) |
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Difference of reproductive strategies and their underlying mechanisms-prolific teleosts and less prolific mammals |
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236 | (1) |
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Toward understanding of a general mechanism of neuroendocrine systems in vertebrates by taking advantage of medaka |
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236 | (1) |
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237 | (1) |
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237 | (8) |
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11 The African turquoise killifish (Nothobranchius furzeri): biology and research applications |
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245 | (6) |
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Large-scale husbandry (including water systems and water parameters) |
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251 | (1) |
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Housing and water parameters |
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251 | (1) |
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252 | (1) |
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Breeding strategies for genome engineering, including egg collection and incubation |
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252 | (5) |
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Breeding and embryo incubation for microinjection and genetic manipulation |
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253 | (1) |
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254 | (1) |
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Hatching of injected eggs and rearing of fry |
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254 | (2) |
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Reagent setup and additional protocols |
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256 | (1) |
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Efficient genome engineering approaches |
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257 | (11) |
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Target selection and synthesis of reagents for CRISPR/Cas9 genome editing |
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258 | (4) |
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262 | (1) |
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263 | (2) |
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Evaluation of editing efficiency (1-2 days) |
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265 | (1) |
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Germline transmission and outcrosses |
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266 | (1) |
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266 | (1) |
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267 | (1) |
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268 | (2) |
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268 | (1) |
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269 | (1) |
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The African turquoise killifish: available toolbox |
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270 | (4) |
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270 | (1) |
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271 | (1) |
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Neurodegeneration and brain functions |
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272 | (1) |
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272 | (1) |
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273 | (1) |
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274 | (1) |
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Summary and future perspectives |
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274 | (1) |
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275 | (3) |
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275 | (2) |
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277 | (1) |
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278 | (1) |
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278 | (11) |
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12 Challenges in keeping annual killifish |
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289 | (1) |
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Available strains and species |
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290 | (2) |
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292 | (5) |
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Behavior and its association to welfare |
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292 | (1) |
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Types of killifish housing |
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293 | (2) |
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Water quality and sanitation |
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295 | (1) |
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295 | (2) |
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297 | (3) |
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297 | (2) |
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Developmental variability |
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299 | (1) |
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Manipulation of embryo development in the laboratory |
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299 | (1) |
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300 | (2) |
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302 | (4) |
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303 | (1) |
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304 | (1) |
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305 | (1) |
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Summary of the challenges |
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306 | (1) |
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306 | (1) |
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306 | (5) |
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13 Mexican tetra (Astyanax mexicanus): biology, husbandry, and experimental protocols |
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Biology and natural history |
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311 | (2) |
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313 | (8) |
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313 | (2) |
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315 | (3) |
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318 | (3) |
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321 | (7) |
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321 | (2) |
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Breeding and embryo handling |
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323 | (4) |
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327 | (1) |
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Animal welfare and health management |
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328 | (6) |
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328 | (1) |
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329 | (1) |
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330 | (1) |
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331 | (1) |
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332 | (2) |
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334 | (6) |
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Visible Implant Elastomer tagging |
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334 | (1) |
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335 | (1) |
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336 | (1) |
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336 | (2) |
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338 | (1) |
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339 | (1) |
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339 | (1) |
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340 | (1) |
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340 | (1) |
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340 | (1) |
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341 | (6) |
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347 | (2) |
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14 The housing, care, and use of a laboratory three-spined stickleback colony |
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349 | (2) |
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Supply, quarantine, and disinfection |
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351 | (1) |
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351 | (1) |
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351 | (1) |
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352 | (5) |
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352 | (1) |
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353 | (1) |
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Stocking density and water quality |
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353 | (1) |
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Photoperiod and temperature |
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354 | (1) |
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355 | (1) |
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355 | (2) |
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Establishing and maintaining a successful colony |
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357 | (9) |
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Annual cycle of temperature and photoperiod |
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358 | (1) |
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Genitors and embryo production |
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359 | (4) |
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363 | (1) |
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364 | (1) |
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365 | (1) |
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366 | (1) |
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366 | (1) |
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366 | (1) |
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Genetic sex determination |
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366 | (1) |
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367 | (1) |
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368 | (1) |
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368 | (5) |
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15 Goldfish (Carassius auratus): biology, husbandry, and research applications |
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373 | (3) |
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376 | (4) |
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376 | (1) |
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377 | (3) |
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Geography and natural habitat |
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380 | (1) |
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381 | (1) |
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381 | (1) |
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381 | (1) |
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381 | (1) |
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382 | (14) |
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Aquatic facility and housing |
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382 | (2) |
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384 | (1) |
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Export and transportation |
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385 | (1) |
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386 | (1) |
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386 | (2) |
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Maintenance/cleaning and disinfection |
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388 | (2) |
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390 | (1) |
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Diseases/pathogens/treatment |
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390 | (6) |
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Goldfish in biomedical research |
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396 | (4) |
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396 | (1) |
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Anesthesia, analgesia, and euthanasia |
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397 | (1) |
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Injections and blood collection |
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397 | (1) |
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Biomedical research applications |
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398 | (2) |
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Conclusions and perspectives |
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400 | (1) |
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400 | (9) |
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16 Danionella translucida, a tankful of new opportunities |
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Biology and ecology of Danionella translucida |
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409 | (2) |
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411 | (1) |
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Development and evolution |
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412 | (1) |
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Animal husbandry: early larval stages |
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413 | (1) |
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Animal husbandry: early stages to adulthood |
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413 | (2) |
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415 | (2) |
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417 | (1) |
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417 | (1) |
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417 | (4) |
| Section 3 Databases |
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17 Fish inventory databases |
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421 | (2) |
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Basic operation principles |
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422 | (1) |
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Fish characterization and grouping |
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423 | (2) |
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424 | (1) |
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424 | (1) |
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Record browsing and spatial tracking |
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425 | (1) |
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425 | (1) |
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Performing actions and monitoring parameters |
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425 | (5) |
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425 | (3) |
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Animal age, productivity, and status |
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428 | (1) |
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428 | (1) |
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Monitoring of the conditions |
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429 | (1) |
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430 | (1) |
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430 | (1) |
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430 | (1) |
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430 | (1) |
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Communication, requesting, and experiment planning |
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431 | (1) |
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431 | (1) |
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Examples of current solutions |
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431 | (1) |
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431 | (1) |
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431 | (1) |
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432 | (1) |
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432 | (1) |
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433 | (1) |
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433 | (2) |
| Index |
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435 | |
Biežāk uzdotie jautājumi par e-grāmatām