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E-grāmata: Liposomes: Methods and Protocols

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  • Formāts: EPUB+DRM
  • Sērija : Methods in Molecular Biology 2622
  • Izdošanas datums: 13-Feb-2023
  • Izdevniecība: Springer-Verlag New York Inc.
  • Valoda: eng
  • ISBN-13: 9781071629543
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  • Formāts: EPUB+DRM
  • Sērija : Methods in Molecular Biology 2622
  • Izdošanas datums: 13-Feb-2023
  • Izdevniecība: Springer-Verlag New York Inc.
  • Valoda: eng
  • ISBN-13: 9781071629543
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This third edition provides new and updated chapters detailing preparation of liposomes, physicochemical characterization of liposomes, lipid analysis, drug encapsulation, surface modification, stimuli response as well as cellular interaction, and biodistribution.  Also included is an updated chapter on the history and evolution of the field of liposomology. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.

 Authoritative and cutting-edge, Liposomes: Methods and Protocols, Third Edition aims to serve as a reference for graduate students, post-doctoral researchers as well as established investigators utilizing lipid-based systems.

From olive oil emulsions to COVID-19 Vaccines - Liposomes Came
First.- Preparation of DRV liposomes.- Preparation of small unilamellar
vesicles using detergent dialysis method.- Thin-film hydration followed by
extrusion method for liposome preparation.- Ethanol injection method for
liposome preparation.- Preparation of giant vesicles with mixed single-tail
and double-tail lipids.- Scalable Liposome Synthesis by High Aspect Ratio
Microfluidic Flow Focusing.- Preparation of doxorubicin liposomes by remote
loading method.- Magnetic thermosensitive liposomes loaded with
Doxorubicin.- Preparation and physical characterization of DNA binding
cationic liposomes.- Tunable pH sensitive lipoplexes.- Solid Lipid
Nanoparticles for Drug Delivery.- Stable Discoidal Bicelles Formulation,
Characterization and Functions .- The post-insertion method for the
preparation of PEGylated liposomes.- Click chemistry for liposome surface
modification.- Surface Modification of Liposomes Using Folic
Acid.- Preparation and Characterization of Trastuzumab Fab conjugated
liposomes (immunoliposomes).- Pyrophosphorylated cholesterol modified
bone-targeting liposomes formulation procedure.- Method of simultaneous
analysis of liposome components using HPTLC/FID.- HPLC-MS/MS Method for
Identification and Quantification of Lipids in Liposomes.- DPH probe method
for liposome-membrane fluidity determination.- Imaging of Liposomes by
Negative Staining Transmission Electron Microscopy and Cryogenic Transmission
Electron Microscopy.- Visualization and characterization of liposomes by
Atomic Force Microscopy (AFM).- Determination of the sub-cellular
distribution of fluorescently labeled liposomes using confocal
microscopy.- Liposome biodistribution via europium complexes.- Quantification
of a fluorescent lipid DOPE-NBD by an HPLC method in biological tissue:
application to study liposomes uptake by human placenta.