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E-grāmata: Lymphoma: Methods and Protocols

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  • Formāts: EPUB+DRM
  • Sērija : Methods in Molecular Biology 971
  • Izdošanas datums: 09-Jan-2013
  • Izdevniecība: Humana Press Inc.
  • Valoda: eng
  • ISBN-13: 9781627032698
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  • Formāts: EPUB+DRM
  • Sērija : Methods in Molecular Biology 971
  • Izdošanas datums: 09-Jan-2013
  • Izdevniecība: Humana Press Inc.
  • Valoda: eng
  • ISBN-13: 9781627032698
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Lymphomas are lymphoid malignancies derived from B or T lymphocytes, and their study has been and still is paradigmatic for many aspects of cancer research. Lymphoma: Methods and Protocols presents and discusses key methods that are used in lymphoma research, partly specific for lymphoma research but often adaptable to the study of other cancers. By covering a broad variety of methods used in lymphoma research, this book will be of interest not only for hematologists, hematopathologists, and immunologists but also for scientists interested in other fields of cancer research as well as human genetics. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Versatile and cutting-edge, Lymphoma: Methods and Protocols serves researchers studying human physiology with the ultimate goal of understanding and controlling these often terrible diseases.

Preface v
Contributors ix
1 Origin and Pathogenesis of B Cell Lymphomas
1(26)
Marc Seifert
Rene Scholtysik
Ralf Kuppers
2 Flow Cytometry for Non-Hodgkin and Classical Hodgkin Lymphoma
27(22)
David Wu
Brent L. Wood
Jonathan R. Fromm
3 Laser-Based Microdissection of Single Cells from Tissue Sections and PCR Analysis of Rearranged Immunoglobulin Genes from Isolated Normal and Malignant Human B Cells
49(16)
Ralf Kuppers
Markus Schneider
Martin-Leo Hansmann
4 PCR-Based Analysis of Rearranged Immunoglobulin or T-Cell Receptor Genes by GeneScan Analysis or Heteroduplex Analysis for Clonality Assessment in Lymphoma Diagnostics
65(28)
Elke Boone
Brenda Verhaaf
Anton W. Langerak
5 Expression Cloning of Human B Cell Immunoglobulins
93(20)
Hedda Wardemann
Juliane Kofer
6 Studying the Replication History of Human B Lymphocytes by Real-Time Quantitative (RQ)-PCR
113(10)
Menno C. van Zelm
Magdalena A. Berkowska
Jacques J.M. van Dongen
7 The Detection of Chromosomal Translocations Involving the Immunoglobulin Loci in B-Cell Malignancies
123(12)
Martin J.S. Dyer
8 Stereotyped B Cell Receptors in B Cell Leukemias and Lymphomas
135(14)
Nikos Darzentas
Kostas Stamatopoulos
9 Flow Cytometric MRD Detection in Selected Mature B-Cell Malignancies
149(26)
Sebastian Bottcher
Matthias Ritgen
Michael Kneba
10 MRD Detection in B-Cell Non-Hodgkin Lymphomas Using Ig Gene Rearrangements and Chromosomal Translocations as Targets for Real-Time Quantitative PCR
175(26)
Christiane Pott
Monika Bruggemann
Matthias Ritgen
Vincent H.J. van der Velden
Jacques J.M. van Dongen
Michael Kneba
11 Enrichment of Methylated DNA by Methyl-CpG Immunoprecipitation
201(12)
Miriam Sonnet
Constance Baer
Michael Rehli
Dieter Weichenhan
Christoph Plass
12 Gene Expression Profile Analysis of Lymphomas
213(14)
Katia Basso
Ulf Klein
13 FISH and FICTION to Detect Chromosomal Aberrations in Lymphomas
227(18)
Maciej Giefing
Reiner Siebert
14 Identification of Pathogenetically Relevant Genes in Lymphomagenesis by shRNA Library Screens
245(20)
Vu N. Ngo
15 Studying MicroRNAs in Lymphoma
265(12)
Joost Kluiver
Izabella Slezak-Prochazka
Anke van den Berg
16 Molecular Methods of Virus Detection in Lymphoma
277(18)
Ruth F. Jarrett
Alice Gallagher
Derek Gatherer
17 High-Throughput RNA Sequencing in B-Cell Lymphomas
295(18)
Wenming Xiao
Bao Tran
Louis M. Staudt
Roland Schmitz
Index 313