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E-grāmata: Macromolecular Crystallography: Deciphering the Structure, Function and Dynamics of Biological Molecules

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This volume is a collection of the contributions presented at the 42nd Erice Crystallographic Course whose main objective was to train the younger generation on advanced methods and techniques for examining structural and dynamic aspects of biological macromolecules. The papers review the techniques used to study protein assemblies and their dynamics, including X-ray diffraction and scattering, electron cryo-electron microscopy, electro nanospray mass spectrometry, NMR, protein docking and molecular dynamics.
A key theme throughout the book is the dependence of modern structural science on multiple experimental and computational techniques, and it is the development of these techniques and their integration that will take us forward in the future.
1 Spatial and Temporal Organisation of Multiprotein Systems of Cell Regulation and Signalling: What Can We Learn from NHEJ System of Double-Strand Break Repair?
1(32)
Qian Wu
Lynn Sibanda
Takashi Ochi
Victor M. Bolanos-Garcia
Tom L. Blundell
Dimitri Y. Chirgadze
2 Co-translational Protein Processing, Folding, Targeting, and Membrane Insertion of Newly Synthesized Proteins
33(10)
Daniel Boehringer
Nenad Ban
3 The Role of Multiple Sequence Repeat Motifs in the Assembly of Multi-protein Complexes
43(8)
David Barford
4 Cryoelectron Tomography or Doing Structural Biology In Situ
51(4)
Wolfgang Baumeister
5 RuvBL1 and RuvBL2 and Their Complex Proteins Implicated in Many Cellular Pathways
55(10)
Sabine Gorynia
Tiago M. Bandeiras
Pedro M. Matias
Filipa G. Pinho
Colin E. McVey
Peter Donner
Maria Armenia Carrondo
6 The Structural Biology of Muscle: Spatial and Temporal Aspects
65(14)
Kenneth C. Holmes
7 Molecular Basis of Allosteric Transitions: GroEL
79(8)
Amnon Horovitz
8 Cell Signalling Through Covalent Modification and Allostery
87(6)
Louise N. Johnson
9 Combining Cryo-EM and X-ray Crystallography to Study Membrane Protein Structure and Function
93(10)
Werner Kuhlbrandt
10 Molecular Mechanisms of DNA Polymerase Clamp Loaders
103(12)
Brian Kelch
Debora Makino
Kyle Simonetta
Mike O'Donnell
John Kuriyan
11 Electron Microscopy of Macromolecular Machines
115(10)
Helen R. Saibil
12 Assembly and Function of the Signal Recognition Particle from Archaea
125(10)
Elisabeth Sauer-Eriksson
Shenghua Huang
Tobias Hainzl
13 Structural Studies of the Functional Complexes of the 50S and 70S Ribosome, a Major Antibiotic Target
135(14)
Thomas A. Steitz
Gregor Blaha
C. Axel Innis
Robin Evans Stanley
David Bulkley
14 Proteopedia: Exciting Advances in the 3D Encyclopedia of Biomolecular Structure
149(14)
Jaime Prilusky
Eran Hodis
Joel L. Sussman
15 Structure Analysis of Biological Macromolecules by Small-Angle X-ray Scattering
163(10)
Dmitri I. Svergun
16 Structural Dynamics of the Vault Ribonucleoprotein Particle
173(10)
Arnau Casanas
Jordi Querol
Ignasi Fita
Nuria Verdaguer
17 Structural Dynamics of Picornaviral RdRP Complexes. Implications for the Design of Antivirals
183(12)
Nuria Verdaguer
Cristina Ferrer-Orta
Esteban Domingo
18 Ribosomes: Ribozymes that Survived Evolution Pressures but Is Paralyzed by Tiny Antibiotics
195
Ada Yonath