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E-grāmata: Mammalian Cell Engineering: Methods and Protocols

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  • Formāts: PDF+DRM
  • Sērija : Methods in Molecular Biology 2312
  • Izdošanas datums: 06-Jul-2021
  • Izdevniecība: Springer-Verlag New York Inc.
  • Valoda: eng
  • ISBN-13: 9781071614419
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Mammalian Cell Engineering: Methods and ProtocolsPalielināt
  • Formāts: PDF+DRM
  • Sērija : Methods in Molecular Biology 2312
  • Izdošanas datums: 06-Jul-2021
  • Izdevniecība: Springer-Verlag New York Inc.
  • Valoda: eng
  • ISBN-13: 9781071614419
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This volume explores the latest engineering methods of mammalian cells that are useful for controlling the performance of engineered mammalian cells for future cell-based therapeutics and for better understanding of complex biological systems. The chapters in this book are organized into five parts. Part One described methods to engineer mammalian cells to sense biologically relevant inputs, such as cell contacts and soluble proteins. Part Two looks at techniques to engineer mammalian cells to sense artificial inputs, such as light and ultrasound. Part Three provides cutting-edge CRISPR-Cas-based methods to carry out highly multiplexed genome editing and spatiotemporally controlled genome editing. Part Four discusses ways to control and engineer biological events in mammalian cells in combination with chemical compounds and systems. Part Five explores techniques to engineer specific mammalian cells in targeted manners. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Comprehensive and authoritative, Mammalian Cell Engineering: Methods and Protocols is a valuable resource that allows scientists to successfully carry out their research, thus ultimately contributing to the future advancement of this field.

Generation of CAR-T Cells by Lentiviral Transduction.- Synthetic
Receptors for Sensing Soluble Molecules with Mammalian Cells.- Engineering
Mammalian Cells to Control Glucose Homeostasis.- Using Engineered Mammalian
Cells for an Epitope-Directed Antibody Affinity Maturation
System.- Purification of Specific Cell Populations Differentiated from Stem
Cells using MicroRNA-Responsive Synthetic Messenger RNAs.- Green
Light-Controlled Gene Switch for Mammalian and Plant Cells.- Sonogenetic
Modulation of Cellular Activities in Mammalian Cells.- Constructing
Smartphone-Controlled Optogenetic Switches in Mammalian Cells.- Constructing
a Smartphone-Controlled Semiautomatic Theranostic System for Glucose
Homeostasis in Diabetic Mice.- Construction of Caffeine-Inducible Gene
Switches in Mammalian Cells.- Multiplexed Genome Engineering with
Cas12a.- Highly Multiplexed Analysis of CRISPR Genome Editing Outcomes in
Mammalian Cells.- Optical Control of Genome Editing by Photoactivatable
Cas9.- Chemogenetic Control of Protein Localization and Mammalian Cell
Signaling by SLIPT.- Engineering Hydrogel Production in Mammalian Cells to
Synthetically Mimic RNA Granules.- AgDD System: A Chemical Controllable
Protein Aggregates in Cells.- Intracellular Unnatural Catalysis Enabled by an
Artificial Metalloenzyme.- Feeder-Free Human Induced Pluripotent Stem Cell
Culture using a DNA Aptamer-Based Mimic of Basic Fibroblast Growth
Factor.- Protocol for de novo Gene Targeting via In Utero
Electroporation.- Magnetically Guided Single Cell Virus Stamping. 
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