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| Preface |
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| About the Companion Website |
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1 Measurement of calcium transient ex vivo |
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3 | (9) |
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2 Confocal imaging of intracellular calcium cycling in isolated cardiac myocytes |
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12 | (8) |
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3 Generating a large animal model of persistent atrial fibrillation |
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20 | (12) |
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4 Confocal imaging of intracellular calcium cycling in the intact heart |
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32 | (9) |
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5 Recording and measurement of action potentials |
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41 | (9) |
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6 Patch-clamp recordings from isolated cardiac myocytes |
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50 | (10) |
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7 Optical mapping of the heart |
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60 | (11) |
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Part 2 Isolation and Maintenance of Primary Stem Cells |
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8 Isolation of colony-forming endothelial progenitor cells |
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71 | (16) |
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9 Cardiac resident stem cells |
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87 | (8) |
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95 | (9) |
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11 Mesenchymal stem cells |
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104 | (6) |
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12 Generation and differentiation of human iPS cells |
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110 | (7) |
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13 Isolation of neonatal and adult rat cardiomyocytes |
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117 | (8) |
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14 Isolation and culture of vascular smooth muscle cells |
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125 | (6) |
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15 Isolation and culture of cardiac endothelial cells |
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131 | (9) |
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16 Isolation and culture of cardiac fibroblasts |
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140 | (6) |
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17 Murine bone marrow transplantation model |
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146 | (3) |
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18 In vitro differentiation and expansion of vascular endothelial cells derived from mouse embryonic stem cells |
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149 | (8) |
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Part 3 Manipulation of the Heart and Vessels in Vivo and ex Vivo |
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157 | (7) |
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20 Transverse aortic constriction: a model to study heart failure in small animals |
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164 | (6) |
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21 Pharmacological models of hypertrophy and failure |
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170 | (7) |
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177 | (10) |
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23 The Langendorff preparation |
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187 | (10) |
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24 Myocarditis and other immunological models of cardiac disease |
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197 | (6) |
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25 Models of pacing-induced heart failure |
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203 | (12) |
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26 Porcine myocardial ischemia models |
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215 | (10) |
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225 | (7) |
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28 Immunohistochemical analysis of cardiac tissue |
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232 | (5) |
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29 A murine model of cardiac arrest by exsanguination |
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237 | (8) |
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Part 4 Small Animal Imaging |
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30 Blood pressure, telemetry, and vascular measurements in the rodent model |
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245 | (5) |
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31 The setting: imaging conscious, sedated, or anesthetized rodents |
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250 | (4) |
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32 Echocardiography: standard techniques (M-mode, two-dimensional imaging, and Doppler) |
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254 | (21) |
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33 Echocardiography: advanced techniques (tissue Doppler, speckle tracking, and three-dimensional imaging) |
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275 | (12) |
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34 In vivo tomographic cardiac imaging: positron emission tomography and magnetic resonance imaging |
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287 | (8) |
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295 | (12) |
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Part 5 Metabolism, Mitochondria, and Cell Death |
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36 Fractionation of cardiomyocytes and isolation of mitochondria |
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307 | (6) |
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37 Assessment of glucose and fatty acid metabolism ex vivo |
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313 | (7) |
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38 Quantification and characterization of atherosclerotic lesions in mice |
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320 | (12) |
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39 Assessment of cell death in the heart |
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332 | (11) |
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40 Assessment of mitochondrial function in isolated cells |
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343 | (8) |
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41 Multinuclear NMR spectroscopy of myocardial energetics and substrate utilization in isolated perfused mouse hearts |
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351 | (8) |
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42 Measurement of reactive oxygen species in cardiovascular disease |
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359 | (12) |
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371 | (7) |
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44 Assessment of cardiomyocyte size |
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378 | (11) |
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Part 6 Manipulation of Gene Expression in Vitro and in Vivo |
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45 Generation of Cre-loxP mouse models for conditional knockout and overexpression of genes in various heart cells |
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389 | (8) |
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46 Modulation of myocardial genes via use of adenoviral vectors and RNA interference approaches |
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397 | (10) |
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47 Overexpression and downregulation of proteins in vitro |
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407 | (9) |
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48 In vivo microRNA studies |
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416 | (9) |
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49 Vascular and cardiac studies in zebrafish |
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425 | (7) |
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50 Vascular and cardiac studies in Drosophila |
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432 | (9) |
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| Index |
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441 | |
Biežāk uzdotie jautājumi par e-grāmatām