E-grāmata: Mesenchymal Stem Cells: Methods and Protocols

These cells, from human and rodent sources, have been under study for over four decades and have been found useful in treating myocardial infarction, stroke, lung diseases, spinal cord injury and neurological disorders, leading to even more paths of research. The contributors of these articles include step-by-step laboratory protocols, materials lists, and tips on avoiding pitfalls in this guide to conducting mesenchymal stem cell research, focusing on the isolation of human multipotential stromal cells (MSCs) from bone marrow and adipose tissue, manipulation of multipotential stromal cells, and isolation and characterization of murine multipotential stromal cells from bone marrow. Topics include a method to isolate and purify human bone marrow stromal stem cells, adipose-derived stem cells, colony-forming unit assays for MSCs, freezing and recovery of MSCs, and assays of MSCs with microrrays. Annotation ©2008 Book News, Inc., Portland, OR (booknews.com)

For over forty years, mesenchymal stem cells (MSCs) have been scrutinized and studied, garnering much attention due to their broad therapeutic efficacy. In Mesenchymal Stem Cells: Methods and Protocols, leaders in the field were assembled to contribute detailed methodologies for the isolation and characterization of human and rodent MSCs. Recently, these vital cells have shown therapeutic benefits in the treatment of myocardial infarction, stroke, lung diseases, spinal cord injury and other neurological disorders, thus promising a boundless future in their study.Cutting edge and easy to use, Mesenchymal Stem Cells: Methods and Protocols is the perfect resource for scientists attempting to pursue this important and ever-developing field of research.