E-grāmata: Metagenomics: Methods and Protocols

Construction of Small-Insert and Large-Insert Metagenomic
Libraries.- Extraction of Total DNA and RNA from Marine Filter Samples and
Generation of a Universal cDNA as Universal Template for Marker Gene
Studies.- Functional Metagenomics as a Tool to Tap into Natural Diversity of
Valuable Biotechnological Compounds.- Functional and Sequence-Specific
Screening Protocols for the Detection of Novel Antimicrobial Resistance Genes
in Metagenomic DNA.- Identification of PKS Gene Clusters from Metagenomic
Libraries Using a Next Generation Sequencing Approach.- Activity-Based
Screening of Metagenomic Fosmid Libraries for Hydrogen-Uptake Enzymes.-
Functional Metagenomics Approach for the Discovery of Novel Genes Encoding
Phosphatase Activity.- Isolation of Genes Encoding Carbon Metabolism Pathways
from Complex Microbial Communities.- Screening Metagenomes for Algae Cell
Wall Carbohydrates Degrading Hydrolases in Enrichment Cultures.- The
PET-Degrading Potential of Global Metagenomes: From InSilico Mining to Active
Enzymes.- High-Throughput Screening for Thermostable Polyester Hydrolases.-
Metagenomic Screening of a Novel PET Esterase via In Vitro Expression
System.- Assigning Functions of Unknown Enzymes by High Throughput Enzyme
Characterization.- Isolation of a Host-Confined Phage Metagenome Allows the
Detection of Phages Both Capable and Incapable of Plaque Formation.-
CRISPR-Cas9 Shaped Viral Metagenomes Associated with Bacillus subtilis.-
Cloning and Expression of Metagenomic DNA in Streptomyces lividans and Its
Subsequent Fermentation for Optimized Production.- DNA-, RNA-, and
Protein-Based Stable-Isotope Probing for High-Throughput Biomarker Analysis
of Active Microorganisms.