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E-grāmata: Methicillin-Resistant Staphylococcus aureus (MRSA) Protocols

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  • Formāts: EPUB+DRM
  • Sērija : Methods in Molecular Biology 391
  • Izdošanas datums: 15-Mar-2008
  • Izdevniecība: Humana Press Inc.
  • Valoda: eng
  • ISBN-13: 9781597454681
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  • Formāts: EPUB+DRM
  • Sērija : Methods in Molecular Biology 391
  • Izdošanas datums: 15-Mar-2008
  • Izdevniecība: Humana Press Inc.
  • Valoda: eng
  • ISBN-13: 9781597454681
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Researchers set out the step-by-step procedures for detecting and investigating resistant strains of a potentially fatal pathogen that has until recently been pretty well controlled by a common drug. They begin each account with an introduction to the method and its purpose, and usually include hints to help fellow laboratory workers avoid lengthy trouble-shooting. The descriptions might also inspire work with other pathogens and other drugs. Annotation ©2008 Book News, Inc., Portland, OR (booknews.com)

MRSA Protocols for Methods in Molecular Biology provides a comprehensive collection of the most up-to-date techniques for the detection and investigation of MRSA. Each chapter begins with a brief introduction to the method and purpose and then goes into detailed protocols for every step of analysis. Several chapters also include a section with tips on individual steps not usually found in methods books. These tips may represent the difference between immediate success and lengthy troubleshooting.Among the topics discussed in this volume are Staphylococcus aureus infections, MIC analysis, ITS-PCR identification, SCCmec analysis, and Biofilm formation. The book also contains helpful case studies, and authors provide insights into the control, prevention, and treatment of infections.Oriented towards routine clinical diagnosis, research, and actual practice for treatment of patients infected by MRSA, MRSA Protocols for Methods in Molecular Biology will make a useful basis for anyone interested in instituting a new method for studying MRSA.

This book provides a comprehensive collection of the most up-to-date techniques for the detection and investigation of MRSA. Each chapter begins with a brief introduction to the method and purpose, and then goes into detailed protocols for every step of analysis. Several chapters also include a section with tips not usually found in methods books. These tips may represent the difference between immediate success and lengthy troubleshooting.

Recenzijas

From the reviews:









"This book has a unique format focussing on detailed experimental protocols. This is particularly useful if you are trying a technique for the first time, troubleshooting a method, or wishing to understand how a technique works in order to critique its use in the literature. there is much to recommend in this book, particularly those who are new to the field and want an introduction to techniques in both basic biology and clinical aspects of MRSA." (Jodi Lindsay, Microbiology Today, May, 2008)



"The book is devoted to the multiple laboratory techniques available for technicians and basic scientists. a good field manual, and clinicians will have an easy to read book on these techniques. describes well the laboratory techniques available for the study of MRSA. Most chapters have illustrations in black and white, which are helpful in the overall discussion, and all chapters have a bibliographic list. it is a good starting point for clinicians and novice scientists in the world of MRSA infections." (Carlos Ernesto Figueroa Castro, Doodys Review Service, May, 2008)



"A unique compilation of 17 chapters aimed chiefly at the scientific investigation of MRSA . This book may also serve as a classic methods reference for those geared towards studying MRSA . In summary, this book represents an excellent reference manual of protocols used to study MRSA, with concise, clear, and well-written introductory sections that can be useful for laboratory investigators and clinicians wishing to get a better understanding of the theory behind scientific methods. is timely in its arrival." (George Sakoulas, Clinical Infectious Diseases, Vol. 46, May, 2008)

Preface v
Contributors ix
Clinical, Epidemiological, and Laboratory Aspects of Methicillin-Resistant Staphylococcus aureus (MRSA) Infections
1(20)
Elizabeth Palavecino
MRSA Case Studies
21(8)
Kurt D. Reed
Mary E. Stemper
Sanjay K. Shukla
Minimum Inhibitory Concentration (MIC) Analysis and Susceptibility Testing of MRSA
29(22)
German Bou
Internal Transcribed Spacer (ITS)-PCR Identification of MRSA
51(8)
Shin-ichi Fujita
Pulsed-Field Gel Electrophoresis of MRSA
59(12)
Kurt D. Reed
Mary E. Stemper
Sanjay K. Shukla
Multilocus Sequence Typing (MLST) of Staphylococcus aureus
71(16)
Nicholas A. Saunders
Anne Holmes
Staphylococcal Cassette Chromosome mec (SCCmec) Analysis of MRSA
87(16)
Teruyo Ito
Kyoko Kuwahara
Keiichi Hiramatsu
Targeted Gene Disruption for the Analysis of Virulence of Staphylococcus aureus
103(10)
J. Ross Fitzgerald
Molecular Analysis of Staphylococcal Superantigens
113(14)
Patrick M. Schlievert
Laura C. Case
Investigation of Biofilm Formation in Clinical Isolates of Staphylococcus aureus
127(18)
James E. Cassat
Chia Y. Lee
Mark S. Smeltzer
Comparative Analysis of Staphylococcal Adhesion and Internalization by Epithelial Cells
145(8)
Xudong Liang
Yinduo Ji
Comparative Analysis of MRSA
153(16)
Fumihiko Takeuchi
Tadashi Baba
Keiichi Hiramatsu
Genomic Analysis of Gene Expression of Staphylococcus aureus
169(10)
Chuanxin Yu
Junsong Sun
Li Zheng
Yinduo Ji
Proteomic Approach to Investigate MRSA
179(22)
Patrice Francois
Alexander Scherl
Denis Hochstrasser
Jacques Schrenzel
Environmental Surveillance for MRSA
201(8)
J. Scott Weese
Control and Prevention of MRSA Infections
209(18)
Liangsu Wang
John F. Barrett
Treatment of Infections Caused by Resistant Staphylococcus aureus
227(32)
Gregory M. Anstead
Gabriel Quinones-Nazario
James S. Lewis II
Index 259