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Methods in Cell Biology, v.52, Methods in Muscle Biology [Hardback]

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  • Formāts: Hardback, 500 pages, height x width: 241x193 mm, weight: 1153 g, b&w illustrations, index
  • Sērija : Methods in Cell Biology Vol 52
  • Izdošanas datums: 01-Oct-1997
  • Izdevniecība: Academic Press Inc
  • ISBN-10: 0125641540
  • ISBN-13: 9780125641548
Citas grāmatas par šo tēmu:
Methods in Cell Biology, v.52, Methods in Muscle BiologyPalielināt
  • Formāts: Hardback, 500 pages, height x width: 241x193 mm, weight: 1153 g, b&w illustrations, index
  • Sērija : Methods in Cell Biology Vol 52
  • Izdošanas datums: 01-Oct-1997
  • Izdevniecība: Academic Press Inc
  • ISBN-10: 0125641540
  • ISBN-13: 9780125641548
Citas grāmatas par šo tēmu:
Permanent link: https://www.kriso.lv/db/9780125641548.html
Keywords:
Methods in Muscle Biology is a comprehensive laboratory guide that details the methods used in the study of muscle biology. The techniques included embrace cell, developmental, and molecular biology, as well as physiology, neurobiology, and medical research.
Contributors xi(4) Preface xv PART I Embryological Analysis of Myogenesis 4(81)
1. Avian Somite Transplantation: A Review of Basic Methods 4(25) Charles P. Ordahl Bodo Christ I. Introduction 4(1) II. Materials 5(6) III. Methods 11(14) IV. Summary and Concluding Remarks 25(1) References 26(3)
2. Myogenesis in the Mouse Embryo 29(24) Margaret Buckingham Giulio Cossu I. Introduction 29(2) II. Manipulation of Genes in the Mouse 31(7) III. Analysis of Gene Expression in the Mouse Embryo 38(5) IV. Experimental Embryology 43(5) V. Perspectives 48(2) References 50(3)
3. Myogenesis in Xenopus Embryos 53(15) John B. Gurdon Patrick Lemaire Timothy J. Mohun I. Fate Maps for Muscle 53(2) II. Embryo Manipulations 55(4) III. Molecular Markers of Muscle Differentiation 59(3) IV. Studies of Xenopus Heart Formation 62(1) References 63(5)
4. Zebrafish: Genetic and Embryological Methods in a Transparent Vertebrate Embryo 68(17) Mark C. Fishman Didier Y. R. Stainier Roger E. Breitbart Monte Westerfield I. Introduction 68(1) II. Genetics 68(2) III. Heart Development 70(2) IV. Skeletal Muscle Development 72(7) References 79(6) PART II Myogenesis in Cell Culture 85(94)
5. Skeletal Muscle Cultures 85(33) Craig Neville Nadia Rosenthal Michael McGrew Natalia Bogdanova Stephen Hauschka I. Introduction 85(1) II. Muscle Cell Cultures 86(27) III. Summary and Future Prospects 113(1) References 114(4)
6. Avian Cardiac Progenitors: Methods for Isolation, Culture, and Analysis of Differentiation 118(15) Maureen Gannon David Bader I. Establishment of Embryonic Axis 118(1) II. Pregastrulation Location of Cardiac Progenitors 119(1) III. Postgastrulation Location of Cardiac Progenitors 119(1) IV. Removal of Embryos from the Egg 120(1) V. Removal of Cardiac Progenitors 121(1) VI. Removal of Endoderm 122(2) VII. Culturing Whole Embryos 124(1) VIII. Culturing Explants of Cardiogenic Mesoderm 125(1) IX. Culturing Primary Cardiomyocytes 126(1) X. Analysis of Differentiation 127(4) References 131(2)
7. Vascular Smooth Muscle Cell Cultures 133(22) Rebecca R. Pauly Claudio Bilato Linda Cheng Robert Monticone Michael T. Crow I. Introduction 133(3) II. Establishment of Cell Cultures 136(5) III. Characterization of Cultured Vascular Smooth Muscle Cells 141(6) IV. Specialized Methods for Human Tissues 147(3) V. Specialized Methods to Maintain the Differentiated State 150(2) VI. Conclusions and Perspectives 152(1) References 153(2)
8. Skeletal Muscle Satellite Cell Cultures 155(24) Ronald E. Allen Constance J. Temm-Grove Shannon M. Sheehan Glenna Rice I. Introduction 155(2) II. Physiological or Developmental Background of Subjects 157(1) III. Monolayer Mass Cultures 158(12) IV. Isolated Single Fiber Cultures 170(3) V. Advantages and Disadvantages of Each Culture System 173(1) VI. Conclusions 174(1) References 175(4) PART III Viral and Cellular Gene Delivery Systems in Muscle 179(96)
9. Retroviral Gene Delivery 179(36) Mark J. Federspiel Stephen H. Hughes I. Introduction 179(12) II. Vector Construction and Virus Propagation 191(5) III. Avian Model 196(5) IV. ALV/Receptor Mouse Model 201(6) V. Summary/Reprise 207(2) References 209(6)
10. The Use of Replication-Defective Retroviruses for Cell Lineage Studies of Myogenic Cells 215(15) Donald A. Fischman Takashi Mikawa I. Introduction 215(1) II. Basic Principles 216(2) III. Practical Procedures 218(5) IV. Application of Retroviruses for Muscle Lineage Analyses in Chicken Embryos 223(2) References 225(5)
11. Adenoviral Gene Delivery 230(31) Thierry Ragot Paule Opolon Michel Perricaudet I. Introduction to Research 230(4) II. Recombinant Adenovirus Design 234(4) III. Practical Methods 238(3) IV. Applications of Adenoviral Gene Transfer to Muscle 241(7) V. Current Problems and Future Prospects 248(8) VI. Conclusion 256(1) References 256(5)
12. Methods for Myoblast Transplantation 261(14) Thomas A. Rando Helen M. Blau I. Introduction 261(1) II. Preparation of Cells 262(2) III. Labeling of Myoblasts in Vitro by Retroviral Mediated Gene Transfer 264(2) IV. Transplantation Techniques 266(2) V. Evaluation of Transplant 268(3) VI. Concluding Remarks 271(1) References 271(4) PART IV Molecular Analysis of Muscle Structure and Function 275(99)
13. Structure-Function Analysis of Cytoskeletal/Contractile Proteins in Avian Myotubes 275(9) H. Lee Sweeney Huisheng Feng I. Introduction 275(1) II. Muscle Cultures 276(3) III. Other Considerations 279(2) References 281(3)
14. Functional and Structural Approaches to the Study of Excitation-Contraction Coupling 284(23) Kurt G. Beam Clara Franzini-Armstrong I. Functional and Structural Approaches to the Identification of e-c Coupling Components 284(12) II. The Dysgenic Muscle Model 296(9) III. Conclusions 305(1) References 305(2)
15. Adenovirus-Mediated Myofilament Gene Transfer into Adult Cardiac Myocytes 307(16) Margaret Westfall Elizabeth Rust Faris Albayya Joseph M. Metzger I. Introduction 307(2) II. Isolation of Adult Cardiac Myocytes and Adenovirus-Mediated Gene Transfer 309(4) III. Production of Recombinant Adenovirus Vectors 313(4) IV. Analysis of Myofilament Protein Expression and Incorporation in Adenovirus-Infected Ventricular Myocytes 317(3) V. Summary and Future Directions 320(1) References 321(2)
16. In Vivo Approaches to Neuromuscular Structure and Function 323(26) Rita J. Balice-Gordon I. Introduction 323(1) II. In Vivo Analysis of Skeletal Muscle and Neuromuscular Innervation 324(13) III. Manipulation of Motor Neurons, Muscle Fibers, and Synapses in Vivo 337(1) IV. In Vivo Observations and Manipulations of Muscle Fibers and Neuromuscular Innervation: Practical Considerations 338(1) V. In Vivo Approaches: New Insights into Neuromuscular Junction Plasticity 339(7) VI. Summary 346(1) References 346(3)
17. Molecular Diversity of Myofibrillar Proteins: Isoforms Analysis at the Protein and mRNA Level 349(25) Stefano Schiaffino Giovanni Salviati I. Myofibrillar Protein Isoforms in Muscle Tissues 349(1) II. Multiple Approaches to Isoform Analysis 350(6) III. Electrophoretic Techniques and Immunoblotting 356(3) IV. Immunohistochemistry and in Situ Hybridization 359(2) V. Other Techniques for mRNA Analysis 361(3) VI. Isoform Analysis and Physiological Studies in Single Muscle Fibers 364(1) VII. Summary and Perspectives 365(2) References 367(7) PART V Molecular Genetic Analysis of Muscle Gene Regulation 374(99)
18. Transgenic Mice: Production and Analysis of Expression 374(31) Alexander Faerman Moshe Shani I. Introduction 374(1) II. Production of Stable Transgenic Mice 375(8) III. Production of Transient Transgenics 383(1) IV. Analysis of Expression at the RNA Level 384(13) V. Analysis of Expression at the Protein Level 397(3) VI. Future Directions 400(1) References 401(4)
19. DNA Transfection of Cultured Muscle Cells 405(18) Craig Neville Nadia Rosenthal Stephen Hauschka I. Introduction 405(1) II. Transient Transfections 406(6) III. Stable Transfections 412(3) IV. Identification of cis-Acting Control Regions with Reporter Assays 415(6) V. Summary and Future Prospects 421(1) References 422(1)
20. DNA- and Adenovirus-Mediated Gene Transfer into Cardiac Muscle 423(17) Alyson Kass-Eisler Leslie A. Leinwand I. Introduction 423(3) II. Methods for Cardiac Injection 426(2) III. Homogenization of Tissue and Assays 428(2) IV. Experimental Design 430(3) V. Future Directions 433(2) References 435(5)
21. Nuclear DNA-Binding Proteins 440(33) Kristen L. Kucharczuk David J. Goldhamer I. Introduction 440(1) II. Nuclear Extract Preparation: Overview and Important Parameters 441(1) III. Nuclear Extract Protocol 442(4) IV. Electromobility Shift Assays: Overview and Important Parameters 446(4) V. EMSA Protocol 450(4) VI. DNase I Protection Assays: Overview and Important Parameters 454(4) VII. DNase I Protection Assay Protocol 458(8) VIII. Relevance of the EMSA and DNase I Protection Assay to Muscle Research 466(5) References 471(2) Index 473(16) Volumes in Series 489