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E-grāmata: Methods in Cyanobacterial Research

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  • Formāts: 251 pages
  • Izdošanas datums: 14-Jun-2024
  • Izdevniecība: CRC Press
  • Valoda: eng
  • ISBN-13: 9781040029084
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Methods in Cyanobacterial ResearchPalielināt
  • Formāts: 251 pages
  • Izdošanas datums: 14-Jun-2024
  • Izdevniecība: CRC Press
  • Valoda: eng
  • ISBN-13: 9781040029084
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This laboratory reference compiles the essential protocols used in cyanobacterial research. Cyanobacteria is a model system for biofuel production and sequestration of carbon dioxide to tackle global climate change due to increasing levels of greenhouse gases. Topics range from identification and growth parameters to biomolecule estimation and isolation. It also covers genome analysis and bioengineering of cyanobacteria for novel products.

Key Features:

  • Provides step-by-step procedures and troubleshooting tips
  • Covers identification, growth parameters and pigment estimation methodologies
  • Describes isolation, characterization and purification of microcystin, phycobiliproteins and scytonemin from cyanobacteria
  • Discusses genomics, proteomics and bioengineering of cyanobacteria from novel products
  • Explains sample preparation and visualization protocol for electron microscopy-based analysis of cyanobacteria                                                                                                             

This collection is useful to students and researchers in life sciences. It is also meant for industry experts who are involved in the production of biofuels, biofertilizers, other value-added products, and carbon sequestration using cyanobacteria.



This laboratory reference compiles the essential protocols used in cyanobacterial research. Cyanobacteria is a model system for biofuel production and sequestration of carbon dioxide to tackle global climate change due to increasing levels of greenhouse gases.

Chapter 1: Evaluating the polyphasic approach in cyanobacterial taxonomy

Chapter 2: Estimation of growth and photosynthetic pigments in cyanobacteria

Chapter 3: Quantitative estimation of total carbohydrate, protein and lipid contents in cyanobacteria

Chapter 4: Isolation and purification of phycobiliproteins

Chapter 5: Methods for estimation of oxidative stress indices in cyanobacteria

Chapter 6: Estimation of glutamine synthetase enzyme

Chapter 7: Estimation of nitrate reductase enzyme

Chapter 8: Estimation of nitrogenase enzyme activity in cyanobacteria

Chapter 9: Isolation and purification of cyanobacterial toxins

Chapter 10: Isolation and purification of scytonemin

Chapter 11: Isolation and purification of mycosporine-like amino acids

Chapter 12: Protein estimation in cyanobacterial samples

Chapter 13: Proteomic analysis of cyanobacteria employing 2-dimensional gel electrophoresis (2-DE)

Chapter 14: LC-MS/MS based proteomics study of cyanobacteria

Chapter 15: Monitoring photosynthetic performance in cyanobacteria by a modulated fluorometer

Chapter 16: Reactive oxygen species measurement in cyanobacteria using 2',7'- dichlorodihydrofluorescein diacetate (DCFH-DA) dye and fluorescence microscope or flow cytometer

Chapter 17: Flow cytometry-based methods for estimating DNA content and live-dead cells in cyanobacteria

Chapter 18: Genomic DNA and RNA extraction from cyanobacteria

Chapter 19: Detection and quantification of DNA damage in cyanobacteria by immunodot- blot assay

Chapter 20: Engineering cyanobacteria for the synthesis of novel products

Chapter 21: Sample preparation and visualization protocol for electron microscopy-based analysis of cyanobacteria

Chapter 22: Cyanobacterial biofertilizer production technology

Chapter 23: Biomass composition measurement and intracellular flux analyses for insights into cyanobacterial metabolism

Chapter 24: In silico genome assembly and functional annotation

Index

Dr. Shailendra P. Singh is an Assistant Professor at the Centre of Advanced Study in Botany, Institute of Science, Banaras Hindu University (BHU), Varanasi, India. He earned his PhD from the Department of Biology, Friedrich-Alexander University, Erlangen-Nuremberg, Germany.

Prof. Dr. Rajeshwar P. Sinha, DAAD Fellowship Awardee and Fellow, Society for Applied Biotechnology, India, is a Professor of Molecular Biology, Centre of Advanced Study in Botany, Institute of Science, Banaras Hindu University (BHU), Varanasi, India.

Prof. Donat-P. Häder is the past-Director of the Botanical Institute and held the chair of Ecophysiology of Plants at the Friedrich-Alexander University, Erlangen-Nürnberg, Germany. He was also the director of the Botanical Garden.
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