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E-grāmata: Microbial Environmental Genomics (MEG)

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  • Formāts: EPUB+DRM
  • Sērija : Methods in Molecular Biology 2605
  • Izdošanas datums: 15-Dec-2022
  • Izdevniecība: Springer-Verlag New York Inc.
  • Valoda: eng
  • ISBN-13: 9781071628713
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Microbial Environmental Genomics (MEG)Palielināt
  • Formāts: EPUB+DRM
  • Sērija : Methods in Molecular Biology 2605
  • Izdošanas datums: 15-Dec-2022
  • Izdevniecība: Springer-Verlag New York Inc.
  • Valoda: eng
  • ISBN-13: 9781071628713
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This volume guides researchers on how to characterize, image rare, and  hitherto unknown taxa and their interactions, to identify new functions and biomolecules and to understand how environmental changes condition the activity and the response of the organisms living with us and in our environment.





Chapters cover different organism types (i.e., archaea, bacteria, fungi, protest, microfauna and microeukaryotes) and propose detailed protocols to produce high quality DNA, to analyse active microbial communities directly involved in complex interactions or processes through stable isotope probing, to identify and characterize of new functional genes, to image in situ interactions and to apply bioinformatics analysis tools to complex metagenomic or RNAseq sequence data. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.





 





Authoritative and cutting-edge, Microbial Environmental Genomics (MEG): Methods and Protocols, Second Edition aims to serve as a primary research reference for researchers in microbiology working to in the expanding field of molecular ecology and environmental genomics.
Metabarcoding Approaches For Soil Eukaryotes, Protists and
Microfauna.- Metabarcoding of the three domains of life in aquatic saline
ecosystems.- Sample preparation for fungal community analysis by
high-throughput sequencing of barcode amplicons.- Characterization of seed
mycobiota using culture-dependent and -independent approaches.- Tissue
Cultivation, Preparation, and Extraction of High Molecular Weight DNA for
Single-Molecule Genome Sequencing of Plant-Associated Fungi.- Analysis of
ancient microbial DNA.- Whole Genome Sequencing of bacterial endophytes from
fresh and preserved plant specimens.- Investigating the bacterial and fungal
communities involved in dead biomass degradation in forest soils.- Stable
isotope probing-RNA strategy to study plant/fungus interactions.- Targeted
16S rRNA Gene Capture by Hybridization and Bioinformatic
Analysis.- Construction of a transposon mutant library in the pathogen
Agrobacterium tumefaciens C58 and identification of genes involved in gall
niche exploitation and colonization.- High-throughput screening of fosmid
libraries for increased identification of novel N-acyl homoserine lactone
degrading enzymes.- Analyzing prokaryotic transcriptomics in the light of
genome data with the MicroScope platform.- MycoCosm, the JGIs Fungal Genome
Portal for Comparative Genomic and Multiomics Data Analyses.- Investigating
endobacteria that thrive within Mucoromycota.- Confocal laser scanning
microscopy approach to investigate plant-fungal interactions.- Visualisation
of fungi during wood colonisation and decomposition by microscopy: from light
to electron microscopy.
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