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E-grāmata: MicroRNA Technologies

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  • Formāts: PDF+DRM
  • Sērija : Neuromethods 128
  • Izdošanas datums: 25-Jul-2017
  • Izdevniecība: Humana Press Inc.
  • Valoda: eng
  • ISBN-13: 9781493971756
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  • Formāts: PDF+DRM
  • Sērija : Neuromethods 128
  • Izdošanas datums: 25-Jul-2017
  • Izdevniecība: Humana Press Inc.
  • Valoda: eng
  • ISBN-13: 9781493971756
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This volume examines different approaches toward understanding the function of microRNA (miRNA) and the tools used to regulate microRNA expression in nervous systems. MicroRNA Technologies also explores newly established techniques to study the role of miRNAs in the nervous system. The chapters in this book cover topics such as: high content screening for looking at the impact of overexpressed miRNAs on primary motor neurons; miRNA expression profiling by PCR array in 2D and 3D neural culture systems; analysis of cortical neuron migration using miRNA silencing; isolating and screening subcellular miRNAs in neurons; and measuring miRNA mediated translational regulation with live cell imaging. The Neuromethods series offers chapters with key advice and procedure specifics to empower the readers to successfully achieve their own scientific and experimental goals.

Cutting-edge and practical, MicroRNA Technologies is a valuable resource for students and researchers who are interested about microRNAs in nervous systems.

Preface to the Series v
Preface vii
Contributors xi
Protocol for miRNA In Situ Hybridization on Mouse Spinal Cord
1(10)
Irit Reichenstein
Eran Hornstein
Protocol for High-Content Screening for the Impact of Overexpressed MicroRNAs on Primary Motor Neurons
11(10)
Tal Yardeni
Eran Hornstein
Quantification of Dicer Activity in Mammalian Cell Lysates Using a Non-radioactive Fluorescence Method
21(8)
Anna Emde
Natalia Rivkin
Mark A. Behlke
Eran Hornstein
Analysis of MicroRNAs and their Potential Targets in Human Embryonic Stem Cell-Derived Neurons Treated with the Anesthetic Propofol
29(14)
Danielle Twaroski
Yasheng Yan
Jessica M. Olson
Mingyu Liang
Zeljko J. Bosnjak
Xiaowen Bai
MicroRNA Expression Profiling by PCR Array in 2D and 3D Differentiated Neural Culture Systems and Target Validation
43(16)
Lara Stevanato
Caroline Hicks
Lavaniya Thanabalasundaram
John D. Sinden
Study of miRNA Function in the Developing Axons of Mouse Cortical Neurons: Use of Compartmentalized Microfluidic Chambers and In Utero Electroporation
59(14)
Patricia P. Garcez
Francois Guillemot
Federico Dajas-Bailador
Functional Analysis of Cortical Neuron Migration Using miRNA Silencing
73(16)
Pierre-Paul Prevot
Marie-Laure Volvert
Alexander Deiters
Laurent Nguyen
Continuous Delivery of Oligonucleotides into the Brain
89(30)
Ilya A. Vinnikov
Andrii Domanskyi
Witold Konopka
Isolating and Screening Subcellular miRNAs in Neuron
119(10)
Min Jeong Kye
Experimental Methods for Functional Studies of microRNAs in Animal Models of Psychiatric Disorders
129(18)
Vladimir Jovasevic
Jelena Radulovic
Isolation and Quantitative Analysis of Axonal Small Noncoding RNAs
147(14)
Hak Hee Kim
Monichan Phay
Soonmoon Yoo
In Ovo Electroporation of miRNA Plasmids to Silence Genes in a Temporally and Spatially Controlled Manner
161(22)
Nicole H. Wilson
Esther T. Stoeckli
Three Dimensional Cell Culture of Human Neural Stem Cells Using Polysaccharide-Based Hydrogels and Subsequent Bioanalyses
183(22)
Geun-woo Jin
Weill Ma
Won H. Suh
Measuring miRNA Mediated Translational Regulation with Live Cell Imaging
205(8)
Min Jeong Kye
Erratum to: Experimental Methods for Functional Studies of microRNAs in Animal Models of Psychiatric Disorders
213(2)
Vladimir Jovasevic
Jelena Radulovic
Index 215