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E-grāmata: Modern Applications of Plant Biotechnology in Pharmaceutical Sciences

, (Head of the Plant Tissue Culture Lab, PDM College of Pharmacy, Bahadurgarh, Haryana, India), (Senior Research Fellow, Department of Plant tissue culture, Jamia Hamdard, New Delhi, India), (Associate Professor, Maharishi Markandeshwar )
  • Formāts: EPUB+DRM
  • Izdošanas datums: 22-Jul-2015
  • Izdevniecība: Academic Press Inc
  • Valoda: eng
  • ISBN-13: 9780128024980
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Modern Applications of Plant Biotechnology in Pharmaceutical SciencesPalielināt
  • Formāts: EPUB+DRM
  • Izdošanas datums: 22-Jul-2015
  • Izdevniecība: Academic Press Inc
  • Valoda: eng
  • ISBN-13: 9780128024980
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Modern Applications of Plant Biotechnology in Pharmaceutical Sciences explores advanced techniques in plant biotechnology, their applications to pharmaceutical sciences, and how these methods can lead to more effective, safe, and affordable drugs.The book covers modern approaches in a practical, step-by-step manner, and includes illustrations, examples, and case studies to enhance understanding.Key topics include plant-made pharmaceuticals, classical and non-classical techniques for secondary metabolite production in plant cell culture and their relevance to pharmaceutical science, edible vaccines, novel delivery systems for plant-based products, international industry regulatory guidelines, and more. Readers will find the book to be a comprehensive and valuable resource for the study of modern plant biotechnology approaches and their pharmaceutical applications.Builds upon the basic concepts of cell and plant tissue culture and recombinant DNA technology to better illustrate the modern and potential applications of plant biotechnology to the pharmaceutical sciencesProvides detailed yet practical coverage of complex techniques, such as micropropogation, gene transfer, and biosynthesisExamines critical issues of international importance and offers real-life examples and potential solutions

Papildus informācija

The only book with a primary focus on the pharmaceutical applications of contemporary plant biotechnology techniques, covering basic mechanisms, principles, methods, and practical examples
About the Lead Author ix
Preface xi
1 History and Scope of Plant Biotechnology
1(30)
Saurabh Bhatia
1.1 Introduction
1(3)
1.2 History of Plant Biotechnology
4(13)
1.3 Scope and Importance of Biotechnology
17(14)
References
25(6)
2 Plant Tissue Culture
31(78)
Saurabh Bhatia
2.1 Introduction
32(1)
2.2 Types of Tissue Culture
32(36)
2.3 Plant Tissue Culture Media
68(18)
2.4 Selection of a Suitable New Medium
86(1)
2.5 Preparation of Media
86(3)
2.6 Callus Induction, Subculture, and Maintenance
89(2)
2.7 Optimization of Media
91(3)
2.8 Maintenance of Cultures Cell Lines
94(2)
2.9 Assessment of their Growth Parameters
96(13)
References
98(11)
3 Laboratory Organization
109(12)
Saurabh Bhatia
Randhir Dahiya
3.1 Introduction
109(1)
3.2 Washing Area
110(2)
3.3 Media Preparation Area
112(3)
3.4 Sterilization Room
115(1)
3.5 Aseptic Transfer Area/Inoculation Room
115(1)
3.6 Culture Room
116(2)
3.7 Data Collection Area
118(1)
3.8 Acclimatization Area
118(1)
3.9 Safety Rules
119(1)
3.10 Design and Layout of Tissue Culture Laboratory
119(1)
3.11 Automation in Plant Tissue Culture
119(2)
References
120(1)
4 Concepts and Techniques of Plant Tissue Culture Science
121(36)
Saurabh Bhatia
Randhir Dahiya
4.1 Introduction
122(2)
4.2 Culturing Techniques
124(5)
4.3 Sterilization Techniques
129(5)
4.4 Genetic Engineering Techniques
134(7)
4.5 Screening Method of Genomic Library
141(4)
4.6 Electrophoresis Techniques
145(3)
4.7 Polymerase Chain Reaction
148(2)
4.8 Microarray
150(1)
4.9 Cryopreservation
150(4)
4.10 Autoradiography
154(1)
4.11 Other Techniques
155(2)
References
155(2)
5 Application of Plant Biotechnology
157(52)
Saurabh Bhatia
5.1 Introduction
158(1)
5.2 Plant Basic Research
159(7)
5.3 Applied Research
166(43)
References
202(7)
6 Somatic Embryogenesis and Organogenesis
209(22)
Saurabh Bhatia
Tanmoy Bera
6.1 Introduction
210(1)
6.2 Somatic Embryogenesis
211(9)
6.3 Synthetic Seed Technology
220(7)
6.4 Organogenesis
227(4)
References
228(3)
7 Classical and Nonclassical Technique for Secondary Metabolite Production in Plant Cell Culture
231(62)
Saurabh Bhatia
Tanmoy Bera
7.1 Introduction
232(2)
7.2 Conventional Methods
234(11)
7.3 Modern or Nonclassical Methods for the Production of Secondary Metabolites
245(33)
7.4 Production of Antitumor Compounds by Plant Cell Culture
278(1)
7.5 Production of Flavoring Compounds through Tissue Culture
279(4)
7.6 Antimicrobial Agents from Plant Cell Culture
283(10)
References
287(6)
8 Plant-Based Biotechnological Products With Their Production Host, Modes of Delivery Systems, and Stability Testing
293(40)
Saurabh Bhatia
Randhir Dahiya
8.1 Introduction
294(1)
8.2 Plant-Based Biotechnological Products with Their Respective Production Host
295(23)
8.3 Different Modes of Delivery Systems Used for Plant-Based Biopharmaceuticals
318(6)
8.4 Stability of Proteins and Peptides
324(9)
References
329(4)
9 Edible Vaccines
333(12)
Saurabh Bhatia
Randhir Dahiya
9.1 Introduction
333(1)
9.2 Production of Edible Vaccines
334(2)
9.3 Mechanism of Action
336(1)
9.4 Advantages and Disadvantages of Edible Vaccines
337(1)
9.5 Most Popular Edible Vaccines Produced by Plants
338(7)
References
342(3)
10 Microenvironmentation in Micropropagation
345(16)
Saurabh Bhatia
Kiran Sharma
10.1 Introduction
346(1)
10.2 Various Problems Faced During Microenvironmentation
347(1)
10.3 Goals of Microenvironmentation
347(1)
10.4 Gas Microenvironment
348(2)
10.5 Light Microenvironment
350(1)
10.6 Water Microenvironment
351(1)
10.7 Temperature Microenvironment
351(1)
10.8 Physical Properties of Culture Vessels
351(2)
10.9 Evaluation of Photosynthetic Capacity in Micropropagated Plants by Image Analysis
353(1)
10.10 Monitoring Gene Expression in Plant Tissue Cultures
353(1)
10.11 Bioreactor Designs
353(1)
10.12 Integrating Automation Technologies with Commercial Micropropagation
354(1)
10.13 Machine Vision and Robotics for the Separation and Regeneration of Plant Tissue Culture
354(1)
10.14 Tissue Culture Gel Firmness: Measurement and Effects on Growth
355(1)
10.15 Commercialized Photoautotrophic Micropropagation System
356(1)
10.16 Closed System for High Quality Transplants Using Minimum Resources
357(1)
10.17 Other Environment Control Systems
358(3)
References
358(3)
11 Micropropagation
361(8)
Saurabh Bhatia
Kiran Sharma
11.1 Introduction
361(1)
11.2 Types of Explants Used in Micropropagation
362(2)
11.3 Methods of Plantlet Production Used in Micropropagation
364(1)
11.4 Stages Involved in Micropropagation
365(1)
11.5 Economic Significance
366(1)
11.6 Applications and Merits of Micropropagation Over Conventional Plant Breeding
367(2)
References
367(2)
12 Laws in Plant Biotechnology
369(24)
Saurabh Bhatia
Randhir Dahiya
12.1 Introduction
370(1)
12.2 Intellectual Property Right Protection
370(3)
12.3 Organization in Intellectual Property Right Protection
373(15)
12.4 Controversial Patent Cases Involving Traditional Knowledge and Genetic Resources
388(5)
References
390(3)
13 Technical Glitches in Micropropagation
393(12)
Saurabh Bhatia
Kiran Sharma
13.1 Introduction
393(3)
13.2 Microbial Contamination
396(1)
13.3 Gaseous Contamination
397(1)
13.4 Cross-Culture Contamination
398(1)
13.5 Browning of the Medium or Phenolic Exudation
398(1)
13.6 Absence of Rooting and Acclimatization
399(1)
13.7 Hyperhydricity
399(1)
13.8 Shoot-Tip Necrosis
400(1)
13.9 Habituation
400(1)
13.10 Tissue Proliferation
401(1)
13.11 Detection of the Contaminants
402(3)
References
403(2)
14 Plant Tissue Culture-Based Industries
405(14)
Saurabh Bhatia
Kiran Sharma
14.1 Introduction
405(1)
14.2 International Scenario
406(5)
14.3 Plant Tissue Culture-Based Industries in India
411(2)
14.4 Recent Technologies
413(2)
14.5 Various Problems Faced During Micropropagation
415(4)
References
415(4)
Subject Index 419
Saurabh Bhatia is Head of the Plant Tissue Culture Lab, PDM College of Pharmacy, Bahadurgarh, Haryana, India.
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