Klientu atbalsts: 27018494

E-grāmata: Molecular Diagnostics

Edited by Nadčge Presneau (Senior Lecturer in Biomedical Sciences, University of Westminster), Edited by Anthony Warford (Emeritus Fellow, Department of Biomedical Sciences, University of Westminster)

Formāts: 344 pages
Sērija : Fundamentals of Biomedical Science
Izdošanas datums: 14-Mar-2019
Izdevniecība: Oxford University Press
Valoda: eng
ISBN-13: 9780191082863

Citas grāmatas par šo tēmu:

Formāts - PDF+DRM
Cena: 50,08 €*
* ši ir gala cena, t.i., netiek piemērotas nekādas papildus atlaides
Ielikt grozā
Pievienot vēlmju sarakstam
Šī e-grāmata paredzēta tikai personīgai lietošanai. E-grāmatas nav iespējams atgriezt un nauda par iegādātajām e-grāmatām netiek atmaksāta.

Formāts: 344 pages
Sērija : Fundamentals of Biomedical Science
Izdošanas datums: 14-Mar-2019
Izdevniecība: Oxford University Press
Valoda: eng
ISBN-13: 9780191082863

Citas grāmatas par šo tēmu:

DRM restrictions

Kopēšana (kopēt/ievietot):

nav atļauts
Drukāšana:

nav atļauts
Lietošana:

Digitālo tiesību pārvaldība (Digital Rights Management (DRM))
Izdevējs ir piegādājis šo grāmatu šifrētā veidā, kas nozīmē, ka jums ir jāinstalē bezmaksas programmatūra, lai to atbloķētu un lasītu. Lai lasītu šo e-grāmatu, jums ir jāizveido Adobe ID. Vairāk informācijas šeit. E-grāmatu var lasīt un lejupielādēt līdz 6 ierīcēm (vienam lietotājam ar vienu un to pašu Adobe ID).

Nepieciešamā programmatūra
Lai lasītu šo e-grāmatu mobilajā ierīcē (tālrunī vai planšetdatorā), jums būs jāinstalē šī bezmaksas lietotne: PocketBook Reader (iOS / Android)

Lai lejupielādētu un lasītu šo e-grāmatu datorā vai Mac datorā, jums ir nepieciešamid Adobe Digital Editions (šī ir bezmaksas lietotne, kas īpaši izstrādāta e-grāmatām. Tā nav tas pats, kas Adobe Reader, kas, iespējams, jau ir jūsu datorā.)

Jūs nevarat lasīt šo e-grāmatu, izmantojot Amazon Kindle.

One of the most notable trends in biomedical science in recent years has been the increasing use of molecular techniques as part of the diagnosis of disease. As such, there is a growing need for students to understand the technological basis of molecular analysis and to have a comprehensive appreciation of their use in diagnosis. Combining coverage of molecular techniques with their application to diagnostic pathology, this book provides students with a thorough and up to date appreciation of the scope of molecular analysis, the principles of the technology used, and thematic diagnostic application.

As part of the Fundamentals of Biomedical Science series, the book provides a unique blend of theory and practice, featuring a range of learning features to help students assimilate the information presented quickly and effectively. It will be relevant to undergraduate students on a wide variety of biomedical pathways, cutting across traditional discipline boundaries to provide a unified overview of molecular diagnostics.

Acknowledgements

vii

An introduction to the Fundamentals of Biomedical Science series

viii

List of Contributors

xii

Introduction

(2)

References

(1)

1 Sample Preparation

(31)

Anthony Warford

Sandra Hing

1.1 Introduction

(1)

1.2 Degradation of cells and tissues

(1)

1.3 Sample preparation and storage

(11)

1.4 Extraction methods

(5)

1.5 Assessment of sample quality

(8)

1.6 Future directions

(1)

Chapter summary

(1)

Further reading

(1)

References

(2)

Useful websites

(1)

Discussion question

(1)

Acknowledgements

(2)

2 The Molecular Laboratory

(15)

Sandra Hing

Anthony Warford

2.1 Introduction

(1)

2.2 Optimal molecular laboratory design

(3)

2.3 Laboratory accreditation and quality management systems

(2)

2.4 Validation and verification

(1)

2.5 Best practice

(4)

2.6 External quality assessment

(1)

2.7 Future directions

(1)

Chapter summary

(1)

References

(1)

Useful websites

(1)

Discussion question

(1)

3 Intact Sample Analysis

(28)

Anthony Warford

Emanuela V. Volpi

3.1 Introduction

(1)

3.2 Technologies

(1)

3.3 Immunocytochemistry

(8)

3.4 Flow cytometry

(6)

3.5 In situ hybridization

(4)

3.6 Controls

(1)

3.7 Future directions

(3)

Chapter summary

(1)

Further reading

(1)

References

(3)

Useful websites

(1)

Discussion question

(1)

4 Homogenate Sample Analysis

(36)

Nadege Presneau

Abdul Hye

Nicholas Ashton

4.1 Introduction

(1)

4.2 Nucleic acid analysis

(18)

4.3 Protein analysis

(12)

4.4 Future directions

108

(5)

Chapter summary

108

(1)

Further reading

109

(1)

References

109

(2)

Useful websites

111

(1)

Discussion questions

112

(1)

5 Sequencing Technologies

113

(23)

Nadege Presneau

Rifat Hamaudi

5.1 Introduction

113

(1)

5.2 First-generation sequencing

114

(5)

5.3 Second- or next-generation sequencing

119

(8)

5.4 Third-generation sequencing

127

(3)

5.5 Future directions

130

(1)

Chapter summary

130

(1)

Further reading

131

(1)

References

131

(3)

Useful websites

134

(1)

Discussion question

135

(1)

6 Molecular Analysis and Interpreting Molecular Data

136

(33)

Rifat Hamoudi

Anthony Warford

6.1 Introduction

136

(1)

6.2 Data analysis of homogenate preparations

137

(18)

6.3 Analysis of intact sample preparations

155

(5)

6.4 Image capture and analysis

160

(1)

6.5 Future directions

161

(1)

Chapter summary

162

(1)

Further reading

162

(1)

References

162

(4)

Useful websites

166

(1)

Discussion questions

166

(3)

7 Recent Technical Advances in Molecular Analysis

169

(37)

Nadege Presneau

Mary Alikian

7.1 Introduction

169

(1)

7.2 Liquid biopsy

170

(14)

7.3 Digital PCR (dPCR)

184

(16)

Chapter summary

200

(1)

Further reading

200

(1)

References

201

(4)

Discussion question

205

(1)

8 Haemopoietic Diseases 1: Leukaemias

206

(41)

Georgina Ryland

Piers Blombery

8.1 An introduction to leukaemia classification

206

(4)

8.2 Acute myeloid leukaemia

210

(12)

8.3 Chronic myeloid leukaemia

222

(5)

8.4 Acute lymphoblastic leukaemia

227

(4)

8.5 Chronic lymphocytic leukaemia (and other chronic lymphoid leukaemias)

231

(9)

8.6 Multiple myeloma

240

(3)

Chapter summary

243

(1)

Further reading

244

(1)

References

245

(1)

Discussion questions

246

(1)

9 Haemopoietic Diseases 2: Lymphoproliferative Disorders

247

(23)

Mark Catherwood

Ken Mills

9.1 Introduction

247

(1)

9.2 Classification of non-Hodgkin lymphomas

248

(2)

9.3 Diagnostic haematopathology

250

(14)

9.4 Future directions

264

(2)

Chapter summary

266

(1)

Further reading

266

(1)

References

266

(1)

Useful websites

266

(3)

Discussion questions

269

(1)

10 Breast Cancer

270

(21)

Mary Falzon

Elaine Borg

Alexandra Saetta

10.1 Introduction

270

(1)

10.2 Histopathological classification

271

(4)

10.3 Grading of tumours

275

(1)

10.4 Sentinel lymph node assessment

276

(1)

10.5 Receptors

277

(3)

10.6 Molecular classification

280

(3)

10.7 Gene expression profiling of breast cancer

283

(2)

10.8 Future directions

285

(2)

Chapter summary

287

(1)

Further reading

287

(1)

References

287

(2)

Useful websites

289

(1)

Discussion question

290

(1)

11 Epithelial Tumours and Melanoma

291

(37)

Manuel Rodriguez-Justo

11.1 Introduction

291

(1)

11.2 Colorectal cancer

292

(12)

11.3 Gastric and gastro-oesophageal adenocarcinoma

304

(3)

11.4 Lung cancer

307

(4)

11.5 Melanoma

311

(2)

11.6 Immuno-checkpoints in epithelial tumours and melanoma

313

(3)

11.7 Future directions

316

(3)

Chapter summary

319

(1)

Further reading

319

(1)

References

320

(6)

Useful websites

326

(1)

Discussion question

327

(1)

12 Mesenchymal Tumours

328

(20)

Fernanda Amary

12.1 Introduction

328

(1)

12.2 Mesenchymal tumours associated with chromosomal translocations

329

(11)

12.3 Mesenchymal tumours associated with gene amplification

340

(3)

12.4 Mesenchymal tumours associated with point mutations

343

(2)

12.5 Sarcomas associated with complex genomic events

345

(1)

Chapter summary

345

(1)

Further reading

345

(1)

References

346

(1)

Discussion questions

347

(1)

13 Prenatal and Neonatal Testing

348

(43)

Claire V. S. Brasted-Pike

13.1 Introduction

348

(1)

13.2 Invasive testing

349

(5)

13.3 Non-invasive testing

354

(7)

13.4 Neonatal testing

361

(2)

13.5 Applications for prenatal testing

363

(9)

13.6 Applications for neonatal testing

372

(5)

13.7 Ethical considerations

377

(3)

13.8 Future directions

380

(1)

Chapter summary

381

(1)

Further reading

382

(1)

References

382

(8)

Useful websites

390

(1)

Discussion questions

390

(1)

Abbreviations

391

(4)

Index

395

Anthony Warford is Emeritus Fellow in the Department of Biomedical Sciences at the University of Westminster, Fellow of the Institute of Biomedical Sciences, and Chartered Scientist. He is the primary author of two scientific papers on tissue microarray, two reviews on in situ hybridisation, and a book chapter on same subject, as well as being a co-author of many other papers. He has lectured internationally on immunohistochemistry and in situ hybridisation.

Nadčge Presneau is Senior Lecturer in the Department of Biomedical Sciences at the University of Westminster. She teaches Molecular Pathology and Cancer Genetics/Genomics at undergraduate and postgraduate levels. Her research focuses principally on developing new bioinformatics approaches to identify driver genes involved in breast cancer using high throughput technologies such as Next Generation Sequencing. She is also interested in the development of new diagnostic tools for cancer as well as biobanking and ethics in translational research.

Biežāk uzdotie jautājumi par e-grāmatām