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Mucins: Methods and Protocols [Mīkstie vāki]

  • Formāts: Paperback / softback, 417 pages, height x width: 254x178 mm, 1 Illustrations, black and white; XIV, 417 p. 1 illus., 1 Paperback / softback
  • Sērija : Methods in Molecular Biology 2763
  • Izdošanas datums: 14-Feb-2025
  • Izdevniecība: Springer-Verlag New York Inc.
  • ISBN-10: 1071636723
  • ISBN-13: 9781071636725
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  • Mīkstie vāki
  • Cena: 127,23 €*
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  • Formāts: Paperback / softback, 417 pages, height x width: 254x178 mm, 1 Illustrations, black and white; XIV, 417 p. 1 illus., 1 Paperback / softback
  • Sērija : Methods in Molecular Biology 2763
  • Izdošanas datums: 14-Feb-2025
  • Izdevniecība: Springer-Verlag New York Inc.
  • ISBN-10: 1071636723
  • ISBN-13: 9781071636725
Citas grāmatas par šo tēmu:

This volume explores the latest advancements in mucin research. The chapters in this book are organized into 8 parts and cover a wide range of topics such as mucin extraction, isolation, physicochemical property analysis, and experimental methods. The chapters also discuss the origins of mucins in jellyfish, feces, saliva and salivary glands, bronchi, stomach, and cervical tract; organic synthesis of peptides glycosylated at specific sites; analysis of mucin gene expression and methylation-specific electrophoresis of genes; imaging of mucin networks by AFM; and experimental methods using supported molecular matrix electrophoresis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and comprehensive, Mucins: Methods and Protocols is a valuable resource for researchers who want to obtain in-depth insight on how to more thoroughly conduct mucin research in their laboratories. 


Preparation of Jellyfish Mucins.- Extraction of Mucin from Rodent Feces
and Determination of O-Linked Oligosaccharide Chain Equivalent Derived from
Fecal Mucin.- Preparation of Soluble Mucin Solutions from the Salivary
Glands.- Isolation of Membrane Bound Mucins from Human Bronchial Epithelial
Cells.- Extraction and Fractionation of Human Gastric Mucins from Gastric
Juice.- Extraction of Mucins from the Mammalian Intestinal Tract.- Supported
Molecular Matrix Electrophoresis.- Supported Immunohistochemical Staining of
Mucins.- Succinylation-Alcian Blue Staining of Mucins on Polyvinylidene
Diuoride Membrane.- Quantitation of Mucin by Densitometry of an Alcian
Blue-Stained Membrane.- Quantitation of MUC5AC and MUC5B by Stable Isotope
Labeling Mass Spectrometry.- Preparation of O-Glycans from Mucins using
Hydrazine Treatment.- Eliminative Oximation of O-glycans from
Mucins.- 9-Fluorenylmethyl Chloroformate Labeling forO-Glycan
Analysis.- Liquid Chromatography and Capillary Electrophoresis Analysis of
2AA-Labeled O-Glycans.- Preparation of Mucin Glycopeptides by Organic
Synthesis.- MALDI-TOF MS/MS Analysis of Permethylated O-Glycan Alditols
Released from Mucins.- Structural Elucidation of Sialylated O-Glycan Alditols
Obtained from Mucins by Mass Spectrometry.- Differential Glycoform Analysis
of MUC1 Derived from Biological Specimens using an Antibody-Overlay Lectin
Microarray.- ISOGlyP: O-Glycosylation Site Prediction using Peptide Sequences
and GALNTs.- Assessment of Mucin-Associated Gene Expression Levels on the
Ocular Surface.- Methylation Specific Electrophoresis.- Expression Analysis
of Genes Corresponding to Mucins and their Glycans from Cervical Tissue using
RNA Sequencing.- Recombinant Production of Glycoengineered Mucins in HEK293-F
Cells.- Analysis of the Interaction between Mucin and Green Fluorescent
Protein (GFP)-Tagged Galectin-2 using a 96-Well Plate.- Solution NMR Analysis
of O-Glycopeptide-Antibody Interaction.- Cultivation of Microorganisms in
Media Supplemented with Mucin Glycoproteins.- Bacterial Enzyme Assay for
Mucin Glycan Degradation.- Measurement of Mucinase Activity.- Adhesion
Inhibition Assay for Helicobacter pylori to Mucin by lactobacillus.- Imaging
of Mucin Networks with Atomic Force Microscopy.- Molecular Dynamics
Simulation and Docking of MUC1 O-Glycopeptide.- Fabrication and
Characterization of Mucin Nanoparticles for Drug Delivery
Applications.- Evaluation of Rheological Properties of Saliva by Determining
the Spinnbarkeit.- Mechanical Characterization of Mucus on Intestinal Tissues
by Atomic Force Microscopy.