Myogenesis: Methods and Protocols Softcover reprint of the original 1st ed. 2019 [Mīkstie vāki]

  • Formāts: Paperback / softback, 351 pages, height x width: 235x155 mm, 64 Illustrations, color; 20 Illustrations, black and white; XII, 351 p. 84 illus., 64 illus. in color., 1 Paperback / softback
  • Sērija : Methods in Molecular Biology 1889
  • Izdošanas datums: 10-Nov-2019
  • Izdevniecība: Humana Press Inc.
  • ISBN-10: 1493994085
  • ISBN-13: 9781493994083
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Myogenesis: Methods and Protocols Softcover reprint of the original 1st ed. 2019
  • Formāts: Paperback / softback, 351 pages, height x width: 235x155 mm, 64 Illustrations, color; 20 Illustrations, black and white; XII, 351 p. 84 illus., 64 illus. in color., 1 Paperback / softback
  • Sērija : Methods in Molecular Biology 1889
  • Izdošanas datums: 10-Nov-2019
  • Izdevniecība: Humana Press Inc.
  • ISBN-10: 1493994085
  • ISBN-13: 9781493994083
Citas grāmatas par šo tēmu:
This detailed volume collects many of the common experimental approaches used to study myogenesis. It covers subjects ranging from isolation and purification protocols, manipulation of muscle cells, transcriptomics and proteomics, metabolism and exercise, and tissue engineering. Presented methods involve different species, including human, bovine, Atlantic salmon, rats, mice, larval zebrafish, and Drosophila melanogaster. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Myogenesis: Methods and Protocols aims to serve as an essential part of many laboratory libraries and to assist researchers throughout the world in revealing the unknowns of myogenesis.
1. Purification of Myogenic Progenitors from Human Muscle Using Fluorescence-Activated Cell Sorting (FACS) Anna Pakula, Janelle M. Spinazzola, and Emanuela Gussoni
2. Electrical Pulse Stimulation of Primary Human Skeletal Muscle Cells Natasa Nikolic and Vigdis Aas
3. Transdifferentiation of Muscle Satellite Cells to Adipose Cells Using CRISPR/Cas9-Mediated Targeting of MyoD Jingjuan Chen, Chao Wang, and Shihuan Kuang
4. Chromatin Immunoprecipitation in Skeletal Myoblasts Vinay Kumar Rao, Shilpa Rani Shankar, and Reshma Taneja
5. Exercising Bioengineered Skeletal Muscle In Vitro: Biopsy to Bioreactor Daniel C. Turner, Andreas M. Kasper, Robert A. Seaborne, Alexander D. Brown, Graeme L. Close, Mark Murphy, Claire E. Stewart, Neil R.W. Martin, and Adam P. Sharples
6. Isolation and Purification of Satellite Cells from Young Rats by Percoll Density Gradient Centrifugation Yuji Matsuyoshi, Mariko Akahoshi, Mako Nakamura, Ryuichi Tatsumi, and Wataru Mizunoya
7. Lentivirus-Mediated RNAi in Skeletal Myogenesis Dongwook Kim, Adriana Reyes-Ordonez, and Jie Chen
8. Adipogenesis from Bovine Precursors Frea Mehta, Ruud Theunissen, and Mark J. Post
9. Transcriptomic Profiling during Myogenesis Alicja Majewska, Tomasz Domoradzki, and Katarzyna Grzelkowska-Kowalczyk
10. Co-Culture Method to Obtain Endothelial Networks within Human Tissue-Engineered Skeletal Muscle Dacha Gholobova, Melanie Gerard, Lisanne Terrie, Linda Desender, Janet Shansky, Herman Vandenburgh, and Lieven Thorrez
11. Interaction between Skeletal Muscle Cells and Extracellular Matrix Proteins Using a Serum Free Culture System Danielle E. Dye, Beverley F. Kinnear, Vishal Chaturvedi, and Deirdre R. Coombe
12. LC-MS Analyses of Lipid Species in Skeletal Muscle Cells and Tissue Marta Moreno-Torres, Jesper F. Havelund, and Nils J. Faergeman
13. siRNA Gene Silencing during C2C12 Myogenesis Alexis Parente, Luce Perie, Laetitia Magnol, Khaled Bouhouche, and Veronique Blanquet
14. Fluorescence-Activated Cell Sorting of Larval Zebrafish Muscle Stem/Progenitor Cells Following Skeletal Muscle Injury Dhanushika Ratnayake and Peter D. Currie
15. Preparation of Proliferated Bovine Primary Skeletal Muscle Cells for Bottom-Up Proteomics by LC-MSMS Analysis Eva Veiseth-Kent, Vibeke Host, and Mona E. Pedersen
16. Myogenesis in Drosophila melanogaster: Dissection of Distinct Muscle Types for Molecular Analysis Anton L. Bryantsev, Lizzet Castillo, Sandy T. Oas, Maria B. Chechenova, Tracy Dohn, and TyAnna L. Lovato
17. Measuring Both Glucose Uptake and Myosin Heavy Chain Isoform Expression in Single Rat Skeletal Muscle Fibers Mark W. Pataky, Edward B. Arias, and Gregory D. Cartee
18. Myoblast Phosphoproteomics as a Tool to Investigate Global Signalling Events during Myogenesis Fiona K. Jones, Gemma E. Hardman, Samantha Ferries, Claire E. Eyers, and Addolorata Pisconti
19. Preparation and Culturing of Atlantic Salmon Muscle Cells for In Vitro Studies Tone-Kari K. Ostbye and Elisabeth Ytteborg
20. RNA Interference Screening for Genes Regulating Drosophila Muscle Morphogenesis Aynur Kaya-Copur and Frank Schnorrer