Preface |
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Contributors |
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1 Introduction to Neurodegenerative Diseases and Related Techniques |
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3 | (6) |
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2 In Vivo and In Vitro Determination of Cell Death Markers in Neurons |
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9 | (14) |
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3 Degenerating Processes Identified by Electron Microscopic Immunocytochemical Methods |
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23 | (40) |
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PART II NEURAL CELL CULTURE TECHNIQUES |
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4 Isolation and Culture of Neurons and Astrocytes from the Mouse Brain Cortex |
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63 | (14) |
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5 Isolation and Culture of Postnatal Spinal Motoneurons |
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77 | (10) |
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6 Converting Human Pluripotent Stem Cells to Neural Tissue and Neurons to Model Neurodegeneration |
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87 | (12) |
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7 Neural Differentiation of Induced Pluripotent Stem Cells |
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99 | (14) |
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PART III MODELS OF NEURODEGENERATION |
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8 Developing Yeast Models of Human Neurodegenerative Disorders |
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113 | (16) |
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9 Modeling Dopamine Neuron Degeneration in Caenorhabditis elegans |
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129 | (20) |
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10 Role of the Proteasome in Fly Models of Neurodegeneration |
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149 | (18) |
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Thomas Schmidt-Glenewinkel |
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11 Modeling Neurodegenerative Diseases in Zebrafish Embryos |
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167 | (18) |
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12 Practical Considerations of Genetic Rodent Models for Neurodegenerative Diseases |
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185 | (10) |
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13 Modeling Focal Cerebral Ischemia In Vivo |
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195 | (16) |
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14 Pharmacological Models of Parkinson's Disease in Rodents |
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211 | (18) |
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15 Behavioral Phenotyping of Mouse Models of Neurodegeneration |
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229 | (12) |
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PART IV NEURODEGENERATIVE DISEASE MECHANISMS: PROTEINOPATHIES |
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16 Characterization of Amyloid Deposits in Neurodegenerative Diseases |
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241 | (18) |
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17 Detection of Protein Aggregation in Neurodegenerative Diseases |
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259 | (14) |
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18 Assessment of Proteasome Impairment and Accumulation/Aggregation of Ubiquitinated Proteins in Neuronal Cultures |
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273 | (26) |
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PART V NEURODEGENERATIVE DISEASE MECHANISMS: MITOCHONDRIA |
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19 Measurements of Threshold of Mitochondrial Permeability Transition Pore Opening in Intact and Permeabilized Cells by Flash Photolysis of Caged Calcium |
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299 | (12) |
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20 Isolation and Functional Assessment of Mitochondria from Small Amounts of Mouse Brain Tissue |
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311 | (14) |
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21 Monitoring Mitophagy in Neuronal Cell Cultures |
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325 | (18) |
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PART VI NEURODEGENERATIVE DISEASE MECHANISMS: BIOLOGICAL FUNCTIONS |
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22 Analysis of Vesicular Trafficking in Primary Neurons by Live Imaging |
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343 | (8) |
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23 Surface Trafficking of Sodium Channels in Cells and in Hippocampal Slices |
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351 | (12) |
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24 Imaging Presynaptic Exocytosis in Corticostriatal Slices |
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363 | (14) |
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25 Microscopic Imaging of Intracellular Calcium in Live Cells Using Lifetime-Based Ratiometric Measurements of Oregon Green BAPTA-1 |
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377 | (14) |
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26 Electrophysiological Characterization of Neuromuscular Synaptic Dysfunction in Mice |
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391 | (10) |
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27 Determination of Neurotransmitter Levels in Models of Parkinson's Disease by HPLC-ECD |
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401 | (16) |
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28 Designing, Performing, and Interpreting a Microarray-Based Gene Expression Study |
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417 | (26) |
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PART VII THERAPEUTIC APPROACHES IN NEURODEGENERATION |
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29 Adeno-Associated Viral Gene Delivery in Neurodegenerative Disease |
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443 | (14) |
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30 Specific Gene Silencing Using RNAi in Cell Culture |
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457 | (22) |
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31 Stem Cell Transplantation for Spinal Cord Neurodegeneration |
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479 | (16) |
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32 Evaluation of Histone Deacetylase Inhibitors as Therapeutics for Neurodegenerative Diseases |
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495 | (14) |
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Index |
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509 | |