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Neurodegeneration: Methods and Protocols 2011 [Hardback]

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  • Formāts: Hardback, 512 pages, height x width: 254x178 mm, weight: 1179 g, XV, 512 p., 1 Hardback
  • Sērija : Methods in Molecular Biology 793
  • Izdošanas datums: 15-Sep-2011
  • Izdevniecība: Humana Press Inc.
  • ISBN-10: 1617793272
  • ISBN-13: 9781617793271
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  • Formāts: Hardback, 512 pages, height x width: 254x178 mm, weight: 1179 g, XV, 512 p., 1 Hardback
  • Sērija : Methods in Molecular Biology 793
  • Izdošanas datums: 15-Sep-2011
  • Izdevniecība: Humana Press Inc.
  • ISBN-10: 1617793272
  • ISBN-13: 9781617793271
Citas grāmatas par šo tēmu:

Neurodegeneration tackles the challenges involved with neurodegenerative disorders, such as Alzheimer's and Parkinson's disease. The text presents cutting-edge analytical and technological approaches to study the mechanisms underlying neurodegenerative processes.



Neurodegenerative disorders, such as Alzheimer's and Parkinson's disease are a growing burden on our society. Understanding and treating these common diseases is one of the major current challenges for the biomedical field. In Neurodegeneration: Methods and Protocols, experts in the field tackle this challenge and present cutting-edge analytical and technological approaches to study the mechanisms underlying neurodegenerative processes. Organizes into five sections, chapters detail protocols for isolating and culturing cells from the nervous system, production and use of animal models, characterization of cell death, analytical tools to study disease mechanisms, and therapeutic approaches for neurodegeneration. Written in the successful Methods in Molecular BiologyT  series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

Authoritative and easily accessible, Neurodegeneration: Methods and Protocols serves as an ideal guide to both novice and professional researchers in the field of neurodegeneration.

Recenzijas

From the reviews:

The authors describe various cell and molecular systems related to neurodegeneration research. there are a lot of protocols and methods described by the book that can be used by medical students, graduate students, junior, and senior investigators. I heartily recommend this book to university and research institute communities working on neurodegeneration and regeneration. (Joseph J. Grenier, Amazon.com, September, 2013)

This is an authoritative collection of manuscripts tailored to bench scientists involved in neurodegenerative research. It is intended for cell biologists, molecular biologists, biochemists, pathologists, and researchers in the field of neurodegeneration. This book is most appropriate for the audience of basic scientists interested in the mechanics of experimental neurodegenerative work. For this audience, the book will find a suitable space on their lab benches. (Joseph I. Sirven, Doodys Book Reviews, April, 2012)

Preface v
Contributors xi
PART I NEURODEGENERATION
1 Introduction to Neurodegenerative Diseases and Related Techniques
3(6)
Hibiki Kawamata
Giovanni Manfredi
2 In Vivo and In Vitro Determination of Cell Death Markers in Neurons
9(14)
Nicole Naniche
Daniela Sau
Piera Pasinelli
3 Degenerating Processes Identified by Electron Microscopic Immunocytochemical Methods
23(40)
Teresa A. Milner
Elizabeth M. Waters
Danielle C. Robinson
Joseph P. Pierce
PART II NEURAL CELL CULTURE TECHNIQUES
4 Isolation and Culture of Neurons and Astrocytes from the Mouse Brain Cortex
63(14)
Hyun Jeong Kim
Jordi Magrane
5 Isolation and Culture of Postnatal Spinal Motoneurons
77(10)
Carol Milligan
David Gifondorwa
6 Converting Human Pluripotent Stem Cells to Neural Tissue and Neurons to Model Neurodegeneration
87(12)
Stuart M. Chambers
Yvonne Mica
Lorenz Studer
Mark J. Tomishima
7 Neural Differentiation of Induced Pluripotent Stem Cells
99(14)
Mark Denham
Mirella Dottori
PART III MODELS OF NEURODEGENERATION
8 Developing Yeast Models of Human Neurodegenerative Disorders
113(16)
Alejandro Ocampo
Antoni Barrientos
9 Modeling Dopamine Neuron Degeneration in Caenorhabditis elegans
129(20)
Michelle L. Tucci
Adam J. Harrington
Guy A. Caldwell
Kim A. Caldwell
10 Role of the Proteasome in Fly Models of Neurodegeneration
149(18)
Chun-Hung Yeh
Marlon Jansen
Thomas Schmidt-Glenewinkel
11 Modeling Neurodegenerative Diseases in Zebrafish Embryos
167(18)
Angela S. Laird
Wim Robberecht
12 Practical Considerations of Genetic Rodent Models for Neurodegenerative Diseases
185(10)
Kindiya Geghman
Chenjian Li
13 Modeling Focal Cerebral Ischemia In Vivo
195(16)
Katherine Jackman
Alexander Kunz
Costantino Iadecola
14 Pharmacological Models of Parkinson's Disease in Rodents
211(18)
Peter Klivenyi
Laszlo Vecsei
15 Behavioral Phenotyping of Mouse Models of Neurodegeneration
229(12)
Magali Dumont
PART IV NEURODEGENERATIVE DISEASE MECHANISMS: PROTEINOPATHIES
16 Characterization of Amyloid Deposits in Neurodegenerative Diseases
241(18)
Ruben Vidal
Bernardino Ghetti
17 Detection of Protein Aggregation in Neurodegenerative Diseases
259(14)
Han-Xiang Deng
Eileen H. Bigio
Teepu Siddique
18 Assessment of Proteasome Impairment and Accumulation/Aggregation of Ubiquitinated Proteins in Neuronal Cultures
273(26)
Natura Myeku
Maria Jose Metcalfe
Qian Huang
Maria Figueiredo-Pereira
PART V NEURODEGENERATIVE DISEASE MECHANISMS: MITOCHONDRIA
19 Measurements of Threshold of Mitochondrial Permeability Transition Pore Opening in Intact and Permeabilized Cells by Flash Photolysis of Caged Calcium
299(12)
Andrey Y. Abramov
Michael R. Duchen
20 Isolation and Functional Assessment of Mitochondria from Small Amounts of Mouse Brain Tissue
311(14)
Christos Chinopoulos
Steven F. Zhang
Bobby Thomas
Vadim Ten
Anatoly A. Starkov
21 Monitoring Mitophagy in Neuronal Cell Cultures
325(18)
Jianhui Zhu
Ruben K. Dagda
Charleen T Chu
PART VI NEURODEGENERATIVE DISEASE MECHANISMS: BIOLOGICAL FUNCTIONS
22 Analysis of Vesicular Trafficking in Primary Neurons by Live Imaging
343(8)
Davide Tampellini
Gunnar K. Gouras
23 Surface Trafficking of Sodium Channels in Cells and in Hippocampal Slices
351(12)
Doo Yeon Kim
Dora M. Kovacs
24 Imaging Presynaptic Exocytosis in Corticostriatal Slices
363(14)
Minerva Y. Wong
David Sulzer
Nigel S. Bamford
25 Microscopic Imaging of Intracellular Calcium in Live Cells Using Lifetime-Based Ratiometric Measurements of Oregon Green BAPTA-1
377(14)
Carli Lattarulo
Diana Thyssen
Kishore V. Kuchibholta
Bradley T Hyman
Brian J. Bacskaiq
26 Electrophysiological Characterization of Neuromuscular Synaptic Dysfunction in Mice
391(10)
Yoshie Sugiura
Fujun Chen
Yun Liu
Weichun Lin
27 Determination of Neurotransmitter Levels in Models of Parkinson's Disease by HPLC-ECD
401(16)
Lichuan Yang
M. Flint Beal
28 Designing, Performing, and Interpreting a Microarray-Based Gene Expression Study
417(26)
Giovanni Coppola
PART VII THERAPEUTIC APPROACHES IN NEURODEGENERATION
29 Adeno-Associated Viral Gene Delivery in Neurodegenerative Disease
443(14)
Peter F. Morgenstern
Roberta Marongiu
Sergei A. Musatov
Michael G. Kaplitt
30 Specific Gene Silencing Using RNAi in Cell Culture
457(22)
Chunxing Yang
Linghua Qiu
Zuoshang Xu
31 Stem Cell Transplantation for Spinal Cord Neurodegeneration
479(16)
Angelo C. Lepore
Nicholas J. Maragakis
32 Evaluation of Histone Deacetylase Inhibitors as Therapeutics for Neurodegenerative Diseases
495(14)
Elisabetta Soragni
Chunping Xu
Andrew Cooper
Heather L. Plasterer
James R. Rusche
Joel M. Gottesfeld
Index 509