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E-grāmata: Next Generation Sequencing in Forensic Science: A Primer [Taylor & Francis e-book]

, (Towson University, Chemistry Department, Maryland, USA)
  • Formāts: 162 pages, 6 Tables, color; 10 Line drawings, black and white; 43 Halftones, black and white; 53 Illustrations, black and white
  • Izdošanas datums: 16-Sep-2021
  • Izdevniecība: CRC Press
  • ISBN-13: 9781003196464
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  • Taylor & Francis e-book
  • Cena: 155,64 €*
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  • Standarta cena: 222,34 €
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Next Generation Sequencing in Forensic Science: A PrimerPalielināt
  • Formāts: 162 pages, 6 Tables, color; 10 Line drawings, black and white; 43 Halftones, black and white; 53 Illustrations, black and white
  • Izdošanas datums: 16-Sep-2021
  • Izdevniecība: CRC Press
  • ISBN-13: 9781003196464
Citas grāmatas par šo tēmu:
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Rokasgrāmatas mājas lapa: https://www.taylorfrancis.com/books/9781003196464

Next Generation Sequencing in Forensic Science: A Primer addresses Next Generation Sequencing (NGS) specific to its application to forensic science.

The book opens with a history of human identity approaches including VNTR, RFLP, STR, and SNP DNA typing. It next discusses the history of sequencing for human DNA typing including: Sanger sequencing, SNaPshot, pyrosequencing, and principles of next generation sequencing. Readers are expected to have as basic understanding of molecular and cellular biology and DNA typing. Chapters present an overview of the forensically-focused AmpliSeq, ForenSeq, Precision ID, PowerSeq and QIAseq panels for human DNA typing using autosomal, Y and X chromosome STRs and SNPs run on the MiSeq FGx and Ion Torrent System. Using these, that authors outline the steps included in DNA extraction and DNA quantitation that are performed prior to preparing libraries with the NGS kits.

The second half of the book details the implementation of ForenSeq and Precision ID to amplify and tag targets to create the library, enrich targets to attach indexes and adaptors, perform library purification and normalization, pool the libraries, and load samples to the cartridge to perform the sequencing on the instrument. Coverage addresses the operation of the MiSeq FGx and Ion Chef including creating a sample list, wash steps, performing NGS, understanding the run feedback files from the instrument, and troubleshooting. ForenSeq and Precision ID panel data analysis are explained including how to analyze and interpret NGS data and output graphs and charts. The book concludes with the mitochondrial chromosome and mitochondrial DNA (mtDNA) sequencing and SNPs analysis including the issue of heteroplasmy. The final chapters review applications of microbial DNA for forensics, NGS in body fluid analysis, and challenges and considerations for future applications.

Key Features:

  • Focuses on human identification using traditional and NGS DNA typing methods targeting short tandem repeats (STRs)

  • Applies the technology and its application to law enforcement investigations and identity and ancestry single nucleotide polymorphisms (SNPs) for investigational leads, mass disaster, and ancestry cases

    • Presents the underlying principles of NGS in clear, easy-to-understand for practitioners and students studying DNA in forensic programs

    • Next Generation Sequencing in Forensic Science

    is the first book to prepare practitioners to utilize and implement this new technology into their lab for casework, highlighting early applications of how NGS results have been used in court. The book can also be utilized for upper-level undergraduate and graduate students taking courses focused on NGS concepts.



    Next Generation Sequencing in Forensic Science prepares practitioners and students to utilize and implement this new technology into their lab casework for forensic investigations, highlighting early applications of how NGS results have been used in court.

    Foreword xi
    Preface xiii
    Acknowledgments xv
    Authors xvii
    List of Figures
    		xix
    List of Tables
    		xxiii
    List of Credits
    		xxv
    List of Abbreviations
    		xxvii
    1 History of DNA-Based Human Identification in Forensic Science
    		1(12)
    1.1 Introduction
    		1(1)
    1.2 Application of DNA Sequencing to Human DNA
    		1(1)
    1.3 History of DNA Typing
    		2(6)
    1.4 Next Generation Sequencing for Forensic DNA Typing
    		8(2)
    1.5 Conclusion
    		10(3)
    Questions
    		10(1)
    References
    		11(2)
    2 History of Sequencing for Human DNA Typing
    		13(18)
    2.1 Introduction
    		13(1)
    2.2 Common Chemistries Used in Sequencing Applications
    		13(4)
    2.2.1 Chain Termination Sequencing
    		13(1)
    2.2.2 Pyrosequencing
    		14(2)
    2.2.3 Sequencing by Ligation
    		16(1)
    2.3 Detection Techniques
    		17(2)
    2.3.1 Fluorescence
    		17(2)
    2.3.2 Pyrosequencing
    		19(1)
    2.3.3 Ion Detection
    		19(1)
    2.4 Sequencing Platforms
    		19(4)
    2.4.1 First-Generation Sequencing Techniques
    		19(1)
    2.4.1.1 Sanger Sequencing
    		19(1)
    2.4.1.2 SNaPShot Sequencing
    		20(1)
    2.4.1.3 Pyrosequencing
    		21(2)
    2.5 Massively Parallel Sequencing
    		23(2)
    2.5.1 Reversible Chain Termination MPS Platforms
    		23(1)
    2.5.2 Ion Detection Platforms
    		23(1)
    2.5.3 Sequencing by Ligation Platforms
    		24(1)
    2.5.4 Single Base Extension Platforms
    		25(1)
    2.5.5 Third-Generation Platforms
    		25(1)
    2.6 NGS Instruments Adopted for Forensic Science
    		25(6)
    Questions
    		28(1)
    References
    		28(3)
    3 Sample Preparation, Standards, and Library Preparation for Next Generation Sequencing
    		31(16)
    3.1 Overview of the NGS Sample Preparation Process
    		31(1)
    3.2 Sample Handling and Processing
    		31(1)
    3.3 DNA Extraction
    		32(2)
    3.4 DNA Quantitation
    		34(1)
    3.5 Library Preparation
    		35(4)
    3.6 Library Purification and Normalization
    		39(2)
    3.7 Multiplexing and Denaturation
    		41(6)
    Questions
    		42(1)
    References
    		42(5)
    4 Performing Next Generation Sequencing
    		47(10)
    4.1 Performing Next Generation Sequencing
    		47(1)
    4.2 Verogen MiSeq FGx® Sequencing
    		47(6)
    4.3 ThermoFisher Ion Torrent™ and Ion PGM Sequencing
    		53(1)
    4.4 The Next Step
    		54(3)
    Questions
    		54(1)
    References
    		55(2)
    5 Next Generation Sequencing Data Analysis and Interpretation
    		57(30)
    5.1 NGS Data Analysis
    		57(1)
    5.2 Verogen Universal Analysis Software
    		58(11)
    5.3 ThermoFisher Converge Software
    		69(5)
    5.4 Phenotype Analysis Using the Erasmus Server
    		74(3)
    5.5 Other Sequence Analysis Software
    		77(1)
    5.6 Additional Tools for Mixture Interpretation
    		78(1)
    5.7 Other NGS Sequence Data Analysis Tools
    		79(1)
    5.8 NGS Validation and Applications
    		80(7)
    Questions
    		83(1)
    References
    		83(4)
    6 Next Generation Sequencing Troubleshooting
    		87(8)
    6.1 Troubleshooting NGS Sequencing
    		87(1)
    6.2 Troubleshooting MiSeq FGx Instrument Failure
    		87(2)
    6.3 Troubleshooting MiSeq FGx Run Failure
    		89(3)
    6.4 Troubleshooting Ion Series Run Failure
    		92(3)
    Questions
    		94(1)
    References
    		94(1)
    7 Mitochondrial DNA Typing Using Next Generation Sequencing
    		95(22)
    7.1 Introduction to Mitochondrial DNA Typing
    		95(1)
    7.2 The Sequence of the Mitochondrial Chromosome
    		96(2)
    7.3 Mitochondrial DNA Typing Methods
    		98(1)
    7.4 Mitochondrial DNA Typing Using Next Generation Sequencing
    		98(4)
    7.5 Mitochondrial Sequence Data Interpretation and Reporting
    		102(5)
    7.6 Recent Reports of Mitotyping Using NGS for Forensic Applications
    		107(1)
    7.7 Mitochondrial Sequence Data and Databases
    		108(9)
    Questions
    		109(1)
    References
    		109(8)
    8 Microbial Applications of Next Generation Sequencing for Forensic Investigations
    		117(20)
    8.1 Introduction to Microbial DNA Profiling
    		117(1)
    8.2 Why NGS?
    		118(1)
    8.3 The Human Microbiome Project
    		118(1)
    8.4 Sampling and Processing
    		118(1)
    8.5 NGS Methodology in Microbial Forensics
    		119(1)
    8.6 Results from the Human Microbiome Project
    		120(1)
    8.7 HMP Applications for Forensic Science
    		121(4)
    8.8 NGS Applications in Geolocation, Autopsy, PMI, and Lifestyle Analysis
    		125(1)
    8.9 Bioinformatic Approaches and Tools
    		126(1)
    8.10 Bioforensics and Biosurveillance
    		127(1)
    8.11 Infectious Disease Diagnostics
    		128(1)
    8.12 NGS Applications in Archeology
    		129(1)
    8.13 Summary of NGS Microbial Sequencing Applications in Forensic Investigation
    		129(8)
    Questions
    		130(1)
    References
    		130(7)
    9 Body Fluid Analysis Using Next Generation Sequencing
    		137(8)
    9.1 Introduction
    		137(1)
    9.2 Epigenetic-Based Tissue Source Attribution
    		137(2)
    9.3 mRNA-Based Tissue Source Attribution
    		139(1)
    9.4 MicroRNA Analysis
    		140(1)
    9.5 The Future of Body Fluid Assays
    		141(4)
    Questions
    		141(1)
    References
    		142(3)
    10 Conclusions and Future Outlook of Next Generation Sequencing in Forensic Science
    		145(14)
    10.1 NGS Is Here
    		145(1)
    10.2 Why NGS?
    		146(1)
    10.3 Ongoing Challenges of Adopting NGS for Forensic Investigations
    		147(5)
    10.4 Early Successes of NGS in Forensic Cases
    		152(2)
    10.5 Summary
    		154(5)
    Questions
    		154(1)
    References
    		154(5)
    Index 159
    Dr. Kelly Elkins is an Associate Professor of Chemistry at Towson University and a founding editor-in-chief of the Journal of Forensic Science Education.

    Dr. Cynthia Zeller is an Associate Professor of Chemistry at Towson University.
    Permanent link: https://www.kriso.lv/db/9781003196464_pe.html
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