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Nuclear Membrane: Methods and Protocols [Hardback]

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  • Formāts: Hardback, 237 pages, height x width: 254x178 mm, 58 Illustrations, color; 4 Illustrations, black and white; VI, 237 p. 62 illus., 58 illus. in color., 1 Hardback
  • Sērija : Methods in Molecular Biology 2958
  • Izdošanas datums: 12-Sep-2025
  • Izdevniecība: Springer-Verlag New York Inc.
  • ISBN-10: 107164713X
  • ISBN-13: 9781071647134
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  • Formāts: Hardback, 237 pages, height x width: 254x178 mm, 58 Illustrations, color; 4 Illustrations, black and white; VI, 237 p. 62 illus., 58 illus. in color., 1 Hardback
  • Sērija : Methods in Molecular Biology 2958
  • Izdošanas datums: 12-Sep-2025
  • Izdevniecība: Springer-Verlag New York Inc.
  • ISBN-10: 107164713X
  • ISBN-13: 9781071647134
Citas grāmatas par šo tēmu:
This book compiles protocols that encompass a diverse range of classical and contemporary approaches, including biochemical techniques, proteomic analyses, microscopic observations, and biophysical measurements. Written in the highly successful Methods in Molecular Biology series format, the chapters include brief introductions to the material, lists of necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and a Notes section which highlights tips on troubleshooting and avoiding known pitfalls.



Authoritative and cutting-edge, The Nuclear Membrane: Methods and Protocols aims to be  a valuable resource for scientists seeking to explore the nuclear structure and functions in the postgenomic era.
Lipidomic profiling of isolated nuclei from mice and cultured cells.-
Profiling plant nuclear envelope composition using subtractive proteomics.-
Ultrastructural visualization of the  nuclearenvelope in HeLa cells.- Imaging
nuclear envelopes using correlative AFM/fluorescence microscopy.- Fast
super-resolution live cell imaging of nuclear membrane-endoplasmic reticulum
dynamicsImmunostaining the yeast nuclear membrane for imaging by
super-resolution fluorescence microscopy.- Visualizing phosphatidic acid and
diacylglycerol at the nuclear envelope in fission yeast.- Measuring molecular
mass densities at subcellular resolution using optical diffraction
tomography.- Quantification and comparison of protein distribution on nuclear
membrane.- Quantifying nuclear shape fluctuations along the cell cycle, with
a focus on early mitosis.- Micropost arrays to model ECM fiber obstacles
during cell migration in confinement.- Use of nucleoporin-conjugated beads to
study the nuclear pore complex assembly on the nuclear membrane.- T4
DNA-induced reconstruction of artificial nuclei in living mouse oocyte.-
 Preparation of nucleoplasmic extract and its application in DNA end
processing.- Liposome floatation assays to study membrane interactions of
nucleoporins.-  Giant unilamellar vesicles (GUVs) to study membrane
interaction of nucleoporins.- One-pot reconstitution of GPCRs into
unilamellar vesicles for fluorescence-based phospholipid scramblase activity
assay.- Identifying genomic DNA sequences near the nuclear lamina using
proximity biotinylation with Ascorbate Peroxidase.-  Pairing LaminB1-DamID
and Immuno-3D-FISH to resolve and verify peripheral genome organization of
adipogenesis.- Measurement of spatial contact map using sequential FISH.