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E-grāmata: Oral Biology: Molecular Techniques and Applications

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  • Formāts: EPUB+DRM
  • Sērija : Methods in Molecular Biology 2588
  • Izdošanas datums: 23-Nov-2022
  • Izdevniecība: Springer-Verlag New York Inc.
  • Valoda: eng
  • ISBN-13: 9781071627808
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  • Formāts: EPUB+DRM
  • Sērija : Methods in Molecular Biology 2588
  • Izdošanas datums: 23-Nov-2022
  • Izdevniecība: Springer-Verlag New York Inc.
  • Valoda: eng
  • ISBN-13: 9781071627808
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This fully revised new edition explores advances in the prevention and treatment of oral diseases. Beyond the updated chapters, the book delves into regenerative biology, gene editing and the use of CRISPR in oral biology, as well as histone acetylation and deacetylation methods, further reflecting advances in the application of molecular techniques to oral biology. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and up-to-date, Oral Biology: Molecular Techniques and Applications, Third Edition serves as an ideal basic resource not only for new researchers but also for experienced scientists wishing to expand their research platform into new areas of this vital field.
RNA Sequencing Analysis of Saliva exRNA.- Proteome Analysis of Oral
Biofluids in Periodontal Health and Disease Using Mass Spectrometry.- Saliva
Diagnosis Using Small Extracellular Vesicles and Salivaomics.- Antioxidant
Micronutrients and Oxidative Stress Biomarkers.- The Oral Microbiota in
Health and Disease: An Overview of Molecular Findings.- The Long and Short of
Genome Sequencing: Using a Hybrid Sequencing Strategy to Sequence Oral
Microbial Genomes.- Microbial Community Profiling Using Terminal Restriction
Fragment Length Polymorphism (T-RFLP) and Denaturing Gradient Gel
Electrophoresis (DGGE).- Bioinformatic Approaches for Describing the Oral
Microbiota.- Adhesion of Yeast and Bacteria to Oral Surfaces.- Quantitative
Analysis of Periodontal Pathogens Using Real Time Polymerase Chain Reaction
(PCR).- Methods to Study Antagonistic Activities Among Oral Bacteria.-
Generation of Multi-Species Oral Bacteria Biofilm Models.- Markerless Genome
Editing in Competent Streptococci.- A Protocol to Produce Genetically Edited
Primary Oral Keratinocytes Using the CRISPR-Cas9 System.- Size-Based Method
for Enrichment of Circulating Tumor Cells from Blood of Colorectal Cancer
Patients.- Strategy for RNA-Seq Experimental Design and Data Analysis.-
Characterization of the Expression and Role of Histone Acetylation and
Deacetylation in Dental Pulp Cells.- Genome-Wide Analysis of Periodontal and
Peri-Implant Cells and Tissues.- Differential Expression, Functional and
Machine Learning Analysis of High-Throughput -Omics Data Using Open-Source
Tools.- Micro-RNA Profiling in Dental Pulp Cell Cultures.- Oral Epithelial
Cell Culture Model for Studying the Pathogenesis of Chronic Inflammatory
Disease.- A Cell Culture Method for the Isolation and Study of Primary Human
Dental Pulp Cells.- Culturing Adipose-Derived Stem Cells Under Serum-Free
Conditions.- Quantitative Real-Time Gene Profiling of Human Alveolar
Osteoblasts Using a One-Step System.- Fabrication and Characterization of
Decellularized Periodontal Ligament Cell Sheet Constructs.-
Immunohistochemistry and Immunofluorescence.- Characterization,
Quantication, and Visualization of Neutrophil Extracellular Traps.- Cell
Seeding on 3D Scaffolds for Tissue Engineering and Disease Modeling
Applications.- Workflow for Fabricating 3D-Printed Resorbable Personalized
Porous Scaffolds for Orofacial Bone Regeneration.- Methacrylated Gelatin as
an On-Demand Injectable Vehicle for Drug Delivery in Dentistry.- In Vitro
Biological Testing of Dental Materials.