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E-grāmata: Photodynamic Therapy: Methods and Protocols

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  • Formāts: EPUB+DRM
  • Sērija : Methods in Molecular Biology 2451
  • Izdošanas datums: 03-May-2022
  • Izdevniecība: Springer-Verlag New York Inc.
  • Valoda: eng
  • ISBN-13: 9781071620991
  • Formāts - EPUB+DRM
  • Cena: 237,34 €*
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  • Formāts: EPUB+DRM
  • Sērija : Methods in Molecular Biology 2451
  • Izdošanas datums: 03-May-2022
  • Izdevniecība: Springer-Verlag New York Inc.
  • Valoda: eng
  • ISBN-13: 9781071620991

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This collection explores state-of-the-art methods and protocols for research on photodynamic therapy (PDT) and its use in a wide range of medical applications, from antiviral to anticancer. Beginning with an extensive section on in vitro and in vivo models, the volume continues with chapters on oxygen-independent photosensitizers, next-generation photosensitization strategies, contemporary insights into the immunomodulatory effects of PDT, antimicrobial effects of PDT, as well as a variety of general biochemical and molecular biological techniques. Written for the highly successful Methods in Molecular Biology series, chapters include the kind of detailed implementation advice that ensures successful results in the lab. 

Thorough and authoritative, Photodynamic Therapy: Methods and Protocols serves as an ideal source of inspiration for both new and established PDT scientists and a guide for designing innovative research programs in this continuously advancing and multidisciplinary field.
Application of Monolayer Cell Cultures for Investigating Basic
Mechanisms of Photodynamic Therapy.- The Negative Impact of Cancer Cell
Nitric Oxide on Photodynamic Therapy.- Microtumor Models as a Preclinical
Investigational Platform for Photodynamic Therapy.- A Microfluidic Model to
Evaluate Photodynamic Therapy Treatment Response in 3D Tumors.- Analysis of
Treatment Effects on Structurally Complex Microtumor Cultures Using a
Comprehensive Image Analysis Procedure.- High-Throughput Examination of
Therapy-Induced Alterations in Redox Metabolism in Spheroid and Microtumor
Models.- Spatiotemporal Tracking of Different Cell Populations in Cancer
Organoid Models for Investigations on Photodynamic Therapy.- Generating Large
Numbers of Pancreatic Microtumors on Alginate-Gelatin Hydrogels for
Quantitative Imaging of Tumor Growth and Photodynamic Therapy Optimization.-
The Chicken Embryo Chorioallantoic Membrane as an In Vivo Model for
Photodynamic Therapy.- Subcutaneous Xenograft Models for Studying PDT In
Vivo.- In Vivo Models for Studying Interstitial Photodynamic Therapy of
Locally Advanced Cancer.- Orthotopic Models of Pancreatic Cancer to Study
PDT.- An Orthotopic Murine Model of Peritoneal Carcinomatosis of Ovarian
Origin for Intraoperative PDT.- Photodynamic Treatments for Disseminated
Cancer Metastases Using Fiber-Optic Technologies.- Stereotaxic Implantation
of F98 Cells in Fischer Rats: A Syngeneic Model to Investigate Photodynamic
Therapy Response in Glioma.- New Generation of Photosensitizers Based on
Inorganic Nanomaterials.- Cytotoxicity of Metal-Based Photoactivated
Chemotherapy (PACT) Compounds.- Photosensitized Oxidation of Intracellular
Targets: Understanding the Mechanisms to Improve the Efficiency of
Photodynamic Therapy.- Inhibition of the HIF-1 Survival Pathway as a Strategy
to Augment Photodynamic Therapy Efficacy.- Strategies for Improving
Photodynamic Therapy through Pharmacological Modulation of the Immediate
Early Stress Response.- Nanobody-Targeted Photodynamic Therapy: Nanobody
Production and Purification.- Conjugation of IRDye Photosensitizers or
Fluorophores to Nanobodies.- In Vitro Assessment of Binding Affinity,
Selectivity, Uptake, Intracellular Degradation, and Toxicity of
Nanobody-Photosensitizer Conjugates.- Assessment of the In Vivo Response to
Nanobody-Targeted PDT through Intravital Microscopy.- Orthotopic Breast
Cancer Model to Investigate the Therapeutic Efficacy of Nanobody-Targeted
Photodynamic Therapy.- Investigation of the Therapeutic Potential of
Nanobody-Targeted Photodynamic Therapy in an Orthotopic Head and Neck Cancer
Model.- Evaluation of the Antitumor Immune Response Following Photofrin-Based
PDT in Combination with the Epigenetic Agent 5-Aza-2-Deoxycytidine.-
Controlling Immunoregulatory Cell Activity for Effective Photodynamic Therapy
of Cancer.- Measuring the Anti-Tumor T-Cell Response in the Context of
Photodynamic Therapy.- Combination of Photodynamic Therapy and Immune
Checkpoint Blockade.- Combination of Photodynamic Therapy and Therapeutic
Vaccination.- In Vitro Potentiation of Antimicrobial Photodynamic
Inactivation by Addition of Potassium Iodide.- In Vivo Potentiation of
Antimicrobial Photodynamic Therapy in a Mouse Model of Fungal Infection by
Addition of Potassium Iodide.- Bioluminescent Models to Evaluate the
Efficiency of Light-Based Antibacterial Approaches.- Photochemical
Internalization as a New Strategy to Enhance Efficacy of Antimicrobial Agents
against Intracellular Infections.- Determination of the Efficiency of
Photodynamic Decontamination of Food.- Super-Resolution Imaging of
Intracellular Lipid Nanocarriers to Study Drug Delivery in Photodynamic
Therapy.- Detection of Paraptosis after Photodynamic Therapy.- Optimal Use of
2“,7“-Dichlorofluorescein Diacetate in Cultured Hepatocytes.