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Plant Gene Regulatory Networks: Methods and Protocols Second Edition 2023 [Mīkstie vāki]

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  • Formāts: Paperback / softback, 382 pages, height x width: 254x178 mm, 63 Illustrations, color; 6 Illustrations, black and white; XII, 382 p. 69 illus., 63 illus. in color., 1 Paperback / softback
  • Sērija : Methods in Molecular Biology 2698
  • Izdošanas datums: 10-Sep-2024
  • Izdevniecība: Springer-Verlag New York Inc.
  • ISBN-10: 1071633562
  • ISBN-13: 9781071633564
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Plant Gene Regulatory Networks: Methods and Protocols Second Edition 2023Palielināt
  • Formāts: Paperback / softback, 382 pages, height x width: 254x178 mm, 63 Illustrations, color; 6 Illustrations, black and white; XII, 382 p. 69 illus., 63 illus. in color., 1 Paperback / softback
  • Sērija : Methods in Molecular Biology 2698
  • Izdošanas datums: 10-Sep-2024
  • Izdevniecība: Springer-Verlag New York Inc.
  • ISBN-10: 1071633562
  • ISBN-13: 9781071633564
Citas grāmatas par šo tēmu:
Permanent link: https://www.kriso.lv/db/9781071633564.html
This second edition details protocols that analyze and explore gene regulatory networks (GRNs). Chapters guide readers through experimental techniques used to study genes and their regulatory interactions in plants, and computational approaches used for the integration of experimental data and bioinformatics-based predictions of regulatory interactions. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.





 





Authoritative and cutting-edge, Plant Gene Regulatory Networks: Methods and Protocols, Second Edition aims to be a useful and practical guide to new researchers and experts looking to expand their knowledge. 
Characterization of gene regulatory networks in plants using new methods
and data types.- Inducible, tissue-specific gene expression in Arabidopsis
using GR-LhG4-mediated trans-activation.- Targeted activation of Arabidopsis
genes by a potent CRISPRAct3.0 system.- Single cell RNA-sequencing in
Arabidopsis root tissues.- Analysis of Chromatin Accessibility, Histone
Modifications, and Transcriptional States in Specific Cell Types Using Flow
Cytometry.- Untargeted proteomics and metabolomics analysis of plant organ
development.- DamID-seq: A DNA methylation method that captures both
transient and stable TF-DNA interactions genome-wide in plant cells.- CUT&Tag
for mapping in vivo protein-DNA interactions in plants.- Identification of
plant transcription factor DNA-binding sites using seq-DAP-seq.- Estimating
DNA-binding specificities of transcription factors using
SELEX-seq.- Immunoprecipitation-mass spectrometry (IP-MS) of protein-protein
interactions of nucleus-localized plant proteins.- Mapping active gene
modules by ChIA-PET in rice.- Building high-confidence gene regulatory
networks by integrating validated TF-target gene interactions using
ConnecTF.- The ChIP-hub resource: towards plantEncode.- A practical guide to
inferring multi-omics networks in plant systems.- Gene regulatory network
modelling using single-cell multi-omics in plants.- Methodology for
Constructing a Knowledgebase for Plant Gene Regulation
Information.- Predicting gene regulatory interactions using natural genetic
variation.- Prediction of transcription factor regulators and gene regulatory
networks in tomato using binding site information.- AGENT for Exploring and
Analyzing Gene Regulatory Networks from Arabidopsis.- A transferable machine
learning framework for predicting transcriptional responses of gene across
species.