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Plant Gene Silencing: Methods and Protocols Second Edition 2022 [Hardback]

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  • Formāts: Hardback, 328 pages, height x width: 254x178 mm, weight: 838 g, 49 Illustrations, color; 7 Illustrations, black and white; XII, 328 p. 56 illus., 49 illus. in color., 1 Hardback
  • Sērija : Methods in Molecular Biology 2408
  • Izdošanas datums: 25-Mar-2022
  • Izdevniecība: Springer-Verlag New York Inc.
  • ISBN-10: 1071618741
  • ISBN-13: 9781071618745
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Plant Gene Silencing: Methods and Protocols Second Edition 2022Palielināt
  • Formāts: Hardback, 328 pages, height x width: 254x178 mm, weight: 838 g, 49 Illustrations, color; 7 Illustrations, black and white; XII, 328 p. 56 illus., 49 illus. in color., 1 Hardback
  • Sērija : Methods in Molecular Biology 2408
  • Izdošanas datums: 25-Mar-2022
  • Izdevniecība: Springer-Verlag New York Inc.
  • ISBN-10: 1071618741
  • ISBN-13: 9781071618745
Citas grāmatas par šo tēmu:
Permanent link: https://www.kriso.lv/db/9781071618745.html
Keywords:

This second edition provides a comprehensive review of various gene silencing methodologies and applications. Chapters detail a historical overview of gene silencing mechanisms in plants, vectors, and strategies available for plant gene silencing, practical applications of gene silencing, bioinformatics tools, and other resources. In addition to these review chapters, this book includes methodology for virus-induced gene silencing (VIGS), understanding plant stress responses using VIGS, miRNA identification, DNA interference, host-induced gene silencing, artificial miRNAs for gene silencing, and high throughput RNAi. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.

 

Authoritative and cutting-edge, Plant Gene Silencing: Methods and Protocols, Second Edition aims to further the understanding of functional relevance of target genes using gene silencing methods and technologies in commercial plant varieties. 

Recent Advances in Plant Gene Silencing Methods.- Strategies for
Efficient RNAi Based Gene Silencing of Viral Genes For Disease Resistance In
Plants.- Genome Editing and Designer Crops for the Future.- In Silico Methods
for the identification of Viral-derived Small interfering RNAs (vsiRNAs) and
their application in Plant genomics.- Barley stripe mosaic virus (BSMV)-Based
Virus-Induced Gene Silencing to Functionally Characterize Genes In Wheat And
Barley.-Virus-induced Gene Silencing in Wheat and Related Monocot
Species.- Virus-Induced Gene Silencing in Sorghum using Brome mosaic
virus.- RTBV-based VIGS Vector for Functional Genomics in Rice: Methodology,
Advances, Challenges, and Future Implications.- Optimization of Tobacco
rattle virus (TRV)-based Virus-Induced Gene Silencing (VIGS) in
Tomato.- Virus-Induced Gene Silencing for Functional Genomics of Specialized
Metabolism in Medicinal  Plants.- VIGS-based Gene Silencing for Assessing
Mineral Nutrient Acquisition.- High throughput Analysis of Gene Function
Under Multiple Abiotic Stresses Using Leaf Disks from Silenced Plants.- A
Method for Developing RNAi-derived Resistance in Cowpea Against
Geminiviruses.- In vitro method for synthesis of large scale ds RNA molecule
as a Novel Plant Protection Strategy.- Fine-tuning Plant Gene Expression with
Synthetic Trans-Acting Small Interfering RNAs.- Trans-Kingdom RNA silencing
in Plant-Fungal Disease Control.- An integrated bioinformatics and functional
approach for miRNA validation.- Development of a Ligation-Independent
Cloning-Based Dual Vector System for RNA Interference in Plants.- Multigene
Transformation Through Cre-lox mediated Site-Specific Integration in
Rice.- An Improvised Hairy Root Transformation Method for Efficient Gene
Silencing in Roots and Nodules of Arachis hypogaea.- A method to reduce
off-targets in CRISPR/Cas9 system in plants.