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E-grāmata: Plant Receptor Kinases: Methods and Protocols

  • Formāts: EPUB+DRM
  • Sērija : Methods in Molecular Biology 1621
  • Izdošanas datums: 31-May-2017
  • Izdevniecība: Humana Press Inc.
  • Valoda: eng
  • ISBN-13: 9781493970636
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  • Formāts: EPUB+DRM
  • Sērija : Methods in Molecular Biology 1621
  • Izdošanas datums: 31-May-2017
  • Izdevniecība: Humana Press Inc.
  • Valoda: eng
  • ISBN-13: 9781493970636
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This is volume presents protocols relevant for plant receptor kinases(PRK) both in development and defence. Chapters guide readers through comprehensive experimental approach for molecular investigations of plant receptor kinases, from the simplest methods for expression and purification of receptor domains to the most advanced methods aiming at understanding the dynamics of receptor complex formation and specificity of signaling pathways. Written in the highly successful Methods

in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Plant Receptor Kinases: Methods and Protocols aims to ensure successful results in the further study of this vital field.

Preface v
Contributors xi
Part I Expression and Purification of PRKs
1 Expression of Plant Receptor Kinases in E. coli
3(18)
Moneeza Akbar Agha
David Lightfoot
Ahmed Jawaad Afzal
2 Expression of Plant Receptor Kinases in Yeast
21(8)
Maria Laura Barberini
Jorge P. Muschietti
3 Expression of Plant Receptor Kinases in Tobacco BY-2 Cells
29(8)
Hidefumi Shinohara
Yoshikatsu Matsubayashi
4 Cell-Free Synthesis of Plant Receptor Kinases
37(10)
Akira Nozawa
Keiichirou Nemoto
Shunsuke Nomura
Satoshi Yamanaka
Kohki Kido
Tatsuya Sawasaki
5 Purification of Plant Receptor Kinases from Plant Plasma Membranes
47(12)
Jin Suk Lee
Part II Ligand Binding and Activation of PRKs
6 Photoaffinity Labeling of Plant Receptor Kinases
59(10)
Hidefumi Shinohara
Yoshikatsu Matsubayashi
7 Quantitative Detection of Oxidative Burst upon Activation of Plant Receptor Kinases
69(10)
Markus Albert
Ursula Furst
Part III In Silico Methods for Identification and Analyses of PRKs
8 Extraction and Curation of Gene Models for Plant Receptor Kinases for Phylogenetic Analysis
79(14)
Aleksia Vaattovaara
Jarkko Salojarvi
Michael Wrzaczek
9 In Silico Prediction of Ligand-Binding Sites of Plant Receptor Kinases Using Conservation Mapping
93(16)
Russell J. S. Orr
Reidunn Birgitta Aalen
Part IV Identification of Enzymatic Functions and Interacting Partners of PRKs
10 Co-immunoprecipitation of Plant Receptor Kinases
109(4)
Maria Laura Barberini
Jorge P. Muschietti
11 Autophosphorylation Assays Using Plant Receptor Kinases Synthesized in Cell-Free Systems
113(8)
Keiichirou Nemoto
Akira Nozawa
Satoshi Yamanaka
Shunsuke Nomura
Kohki Kido
Tatsuya Sawasaki
12 Investigation of Autophosphorylation Sites of Plant Receptor Kinases and Phosphorylation of Interacting Partners
121(10)
Matthew R. Meyer
Shweta Shah
A. Gururaj Rao
13 In Vitro Assessment of Guanylyl Cyclase Activity of Plant Receptor Kinases
131(10)
Misjudeen Raji
Chris Gehring
14 BiFC Assay to Detect Calmodulin Binding to Plant Receptor Kinases
141(10)
Cornelia Fischer
Margret Sauter
Petra Dietrich
15 Pull-down Assay to Characterize Ca2+/Calmodulin Binding to Plant Receptor Kinases
151(12)
Christine Kaufmann
Margret Sauter
Part V Imaging of PRK Dynamics
16 The Use of FRET/FLIM to Study Proteins Interacting with Plant Receptor Kinases
163(14)
Stefanie Weidtkamp-Peters
Yvonne Stahl
17 Detection and Analyses of Endocytosis of Plant Receptor Kinases
177(14)
Jenna Loiseau
Silke Robatzek
Index 191