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E-grāmata: Plant Signal Transduction: Methods and Protocols

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  • Formāts: PDF+DRM
  • Sērija : Methods in Molecular Biology 1363
  • Izdošanas datums: 17-Nov-2015
  • Izdevniecība: Humana Press Inc.
  • Valoda: eng
  • ISBN-13: 9781493931156
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Plant Signal Transduction: Methods and ProtocolsPalielināt
  • Formāts: PDF+DRM
  • Sērija : Methods in Molecular Biology 1363
  • Izdošanas datums: 17-Nov-2015
  • Izdevniecība: Humana Press Inc.
  • Valoda: eng
  • ISBN-13: 9781493931156
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This fully updated volume reflects the spectacular advances in our knowledge of signal transduction pathways with a selection of ‘classic’ as well as newly developed approaches. These detailed approaches expand into the fields of molecular biology, biochemistry, physiology, cell biology, genetics, and genomics. Written in the highly successfulMethods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Practical and up-to-date, Plant Signal Transduction: Methods and Protocols, Second Edition serves as an ideal guide for researchers exploring the vast array of signals produced by plants to ensure their survival.

Preface vii
Contributors xiii
1 Suppressor Screens in Arabidopsis
1(8)
Xin Li
Yuelin Zhang
2 Quantitative Reverse Transcription-qPCR-Based Gene Expression Analysis in Plants
9(16)
Heithem Ben Abdallah
Petra Bauer
3 DNA-Binding Factor Target Identification by Chromatin Immunoprecipitation (ChIP) in Plants
25(12)
David Pose
Levi Yant
4 Identification of Genes Responsible for Natural Variation in Volatile Content Using Next-Generation Sequencing Technology
37(10)
Iraida Amaya
Jeremy Pillet
Kevin M. Folta
5 Micro-Tom Tomato as an Alternative Plant Model System: Mutant Collection and Efficient Transformation
47(10)
Masahito Shikata
Hiroshi Ezura
6 Culture of the Tomato Micro-Tom Cultivar in Greenhouse
57(8)
Christophe Rothan
Daniel Just
Lucie Fernandez
Isabelle Atienza
Patricia Ballias
Martine Lemaire-Chamley
7 Virus-Induced Gene Silencing as a Tool to Study Tomato Fruit Biochemistry
65(14)
Elio Fantini
Giovanni Giuliano
8 A Simplified and Rapid Method for the Isolation and Transfection of Arabidopsis Leaf Mesophyll Protoplasts for Large-Scale Applications
79(10)
Arnaldo L. Schapire
L. Maria Lois
9 Preparation of Epidermal Peels and Guard Cell Protoplasts for Cellular, Electrophysiological, and -Omics Assays of Guard Cell Function
89(34)
Mengmeng Zhu
Byeong Wook Jeon
Sisi Geng
Yunqing Yu
Kelly Balmant
Sixue Chen
Sarah M. Assmann
10 Application of Variable Angle Total Internal Reflection Fluorescence Microscopy to Investigate Protein Dynamics in Intact Plant Cells
123(10)
Yinglang Wan
Yiqun Xue
Ruili Li
Jinxing Lin
11 Immunoprecipitation of Plasma Membrane Receptor-Like Kinases for Identification of Phosphorylation Sites and Associated Proteins
133(12)
Yasuhiro Kadota
Alberto P. Macho
Cyril Zipfel
12 Yeast Three-Hybrid System for the Detection of Protein-Protein Interactions
145(10)
Natsumi Maruta
Yuri Trusov
Jose R. Botella
13 Cautions in Measuring In Vivo Interactions Using FRET and BiFC in Nicotiana benthamiana
155(20)
Meral Tunc-Ozdemir
Yan Fu
Alan M. Jones
14 Analysis of Protein---Lipid Interactions Using Purified C2 Domains
175(14)
Jessica Perez-Sancho
Arnaldo L. Schapire
Miguel A. Botella
Abel Rosado
15 Assessing Kinase Activity in Plants with In-Gel Kinase Assays
189(10)
Pengcheng Wang
Jian-Kang Zhu
16 Analyses of Plant UDP-Dependent Glycosyltransferases to Identify Their Volatile Substrates Using Recombinant Proteins
199(10)
Yusuke Kamiyoshihara
Denise M. Tieman
Harry J. Klee
17 Competitive Index: Mixed Infection-Based Virulence Assays for Genetic Analysis in Pseudomonas syringae-Plant Interactions
209(10)
Alberto P. Macho
Jose S. Rufian
Javier Ruiz-Albert
Carmen R. Beuzon
18 Simplified Assays for Evaluation of Resistance to Alternaria brassicicola and Turnip Mosaic Virus
219(10)
Yuri Trusov
Ralf G. Dietzgen
Natsumi Maruta
Jose R. Botella
19 Simultaneous Determination of Plant Hormones by GC-TOF-MS
229(10)
Jose G. Vallarino
Sonia Osorio
Index 239
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