E-grāmata: Plant Stress Tolerance: Methods and Protocols

Strategies of Molecular Signal Integration for Optimized Plant Acclimation to Stress Combinations.- MNase-Seq Analysis for Identifying Stress-Altered Nucleosome Occupancy in Plants.- MeRIP-Seq for Identifying Stress-Responsive Transcriptome-Wide m6A Profiles in Plants.- Isolation and Visualization of Plant Stress Granule-Associated Components via On-Beads Digestion and Co-Localization Analysis.- Determination of Differential Alternative Splicing Under Stress Conditions.- A Method to Quantitatively Examine Heat Stress-Induced Alternative Splicing in Plants by RNA-Seq and RT-PCR.- Thiol Redox Proteomics for Identifying Redox-Sensitive Cysteine Residues Within the Protein of Interest During Stress.- Detection of Stress-Induced Changes in Subcellular Protein-Distribution.- Validation of the Interaction Between Stress-Responsive LncRNAs and RNA-Binding Proteins In Vitro.- Measurement of Photorespiratory Cycle Enzyme Activities in Leaves Exposed to Abiotic Stress.- In Vitro Kinase Assay with Recombinant SnRK2s: An Example for Assaying Stress-Responsive Kinases in Plants.- Identification of Stress-Responsive Metabolites in Plants Using an Untargeted Metabolomics Approach.- Measurement of Reactive Oxygen Species and Nitric Oxide from Tomato Plants in Response to Abiotic and Biotic Stresses.- Reactive Oxygen Species (ROS) Measurement in Arabidopsis Guard Cells in Response to Biotic and Abiotic Stresses.- Detection of Reactive Oxygen Species in Plant Root Immunity.- Studying Thermopriming-Mediated Short- and Long-Term Acquired Thermotolerance in Arabidopsis thaliana.- X-Ray Film Contact Print Method for Analyzing Differential Expression of Plant Protease Inhibitors Under Abiotic Stress.- Isolation and Identification of Growth-Promoting Bacteria from Plants Growing Under Abiotic Stresses.- Functional Characterization and Localization of Plant-Growth Promoting Bacteria Grown Under Stressful Conditions.- Generating Mutant for a Stress-Responsive Gene in Rice Using CRISPR-Cas9 System.