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Practical Bacteriology [Hardback]

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  • Formāts: Hardback, 196 pages, height x width: 240x160 mm, weight: 475 g
  • Izdošanas datums: 30-Jan-2017
  • Izdevniecība: Alpha Science International Ltd
  • ISBN-10: 1783322888
  • ISBN-13: 9781783322886
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  • Cena: 38,46 €
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Practical BacteriologyPalielināt
  • Formāts: Hardback, 196 pages, height x width: 240x160 mm, weight: 475 g
  • Izdošanas datums: 30-Jan-2017
  • Izdevniecība: Alpha Science International Ltd
  • ISBN-10: 1783322888
  • ISBN-13: 9781783322886
Citas grāmatas par šo tēmu:
Permanent link: https://www.kriso.lv/db/9781783322886.html
Contains impressive experiments on bacteriology research ideas that have not been included in any previous practical book. The experiments are written in brief and the procedures summarized in appealing and uncomplicated language.

Contains impressive experiments on bacteriology research ideas that have not included in any practical book so far. The book has been written according to the application of bacteria in various fields of biotechnology.

The experiments have been written in brief and the procedures summarized in an appealing and uncomplicated language. Appendices of the book provide a broad knowledge in bacteriology to the readers. The subject matter in Practical Bacteriology contains meaningful and essential experiments in order to carry out novel and competent research.

Contains impressive experiments on bacteriology research ideas that have not included in any practical book so far. The book has been written according to the application of bacteria in various fields of biotechnology.

The experiments have been written in brief and the procedures summarized in an appealing and uncomplicated language. Appendices of the book provide a broad knowledge in bacteriology to the readers. The subject matter in Practical Bacteriology contains meaningful and essential experiments in order to carry out novel and competent research.
Preface iii
Introduction v
1 Glassware
1(3)
2 Materials
4(2)
3 Instruments
6(5)
4 Apparatus
11(2)
5 Isolation and Screening Techniques
13(10)
5.1 Serial Dilution Technique
13(1)
5.2 Preparation of Liquid Media
14(1)
5.3 Preparation of Solid Media
15(1)
5.4 Spread Plate Technique
16(1)
5.5 Colony Forming Unit (CFU)
17(1)
5.6 Bacterial Colony Morphology
18(1)
5.7 Pour Plate Technique
19(1)
5.8 Streaking Technique
20(1)
5.9 Preparation of Agar Slant Culture
21(1)
5.10 Preparation of Bacterial Glycerol Stock
22(1)
6 Staining Techniques
23(9)
6.1 Simple Staining
23(1)
6.2 Negative Staining
24(1)
6.3 Flagella Staining
25(1)
6.4 Capsule Staining
26(1)
6.5 Endospore Staining
27(1)
6.6 Gram Staining Technique
28(2)
6.7 Acid-Fast Staining
30(2)
7 Motility Test
32(3)
7.1 Preparation of Agar Stab Culture
32(1)
7.2 Wet Mount Technique
32(1)
7.3 Hanging Drop Technique
33(2)
8 Biochemical Test
35(11)
8.1 Indole Test
35(1)
8.2 Methyl Red Test
36(1)
8.3 Voges-Proskauer Test
37(1)
8.4 Citrate Utilization Test
38(1)
8.5 Urease Test
39(1)
8.6 Catalase Test
40(1)
8.7 Oxidase Test
41(1)
8.8 Phenylalanine Deaminase Test
42(1)
8.9 Hydrogen Sulphide Production Test
43(1)
8.10 Lactose Fermentation Test
44(2)
9 Bacterial Growth Curve
46(2)
10 Antimicrobial Susceptibility Test
48(12)
10.1 Antibacterial Activity Test
48(3)
10.2 Antibiotic Susceptibility Test
51(1)
10.3 Multiple Antibiotic Resistance (MAR) Index
52(1)
10.4 Minimum Inhibitory Concentration
53(1)
10.5 Maximum Tolerable Concentration
54(1)
10.6 Antagonism
55(1)
10.7 Preliminary Screening for Antibiotic Production
56(1)
10.8 Activity Index
57(1)
10.9 Percentage Susceptibility Change
58(2)
11 Antioxidant Activity
60(3)
12 Alpha-Glucosidase Inhibition Test
63(2)
13 Isolation of Bacterial Species
65(9)
13.1 Isolation of Escherichia sp.
65(1)
13.2 Isolation of Pseudomonas sp.
66(1)
13.3 Isolation of Bacillus sp.
67(1)
13.4 Isolation of Actinomycetes
68(1)
13.5 Isolation of Rhizobium sp.
69(1)
13.6 Isolation of Nitrogen Fixing Bacteria
70(4)
14 Enzyme Assay
74(24)
14.1 Isolation of Amylase Producing Bacteria
74(2)
14.2 Isolation of Protease Producing Bacteria
76(3)
14.3 Isolation of Lipase Producing Bacteria
79(3)
14.4 Isolation of Cellulase Producing Bacteria
82(2)
14.5 Isolation of Keratinase Producing Bacteria
84(2)
14.6 Isolation of Polygalacturonase Producing Bacteria
86(1)
14.7 Isolation of Pectinase Producing Bacteria
87(2)
14.8 Isolation of L-Asparaginase Producing Bacteria
89(2)
14.9 Isolation of Beta-Galactosidase Producing Bacteria
91(1)
14.10 Isolation of Xylanase Producing Bacteria
92(2)
14.11 Isolation of Chitinase Producing Bacteria
94(2)
14.12 Isolation of Nattokinase Producing Bacteria
96(2)
15 Bacteriology Research Ideas
98(27)
15.1 Protein Concentration Determination
98(1)
15.2 Specific Enzyme Activity
99(1)
15.3 Purification of Bacterial Enzyme
100(2)
15.4 Relative Percentage Inhibition
102(1)
15.5 Antiquorum Sensing Activity
102(1)
15.6 Isolation of Phosphate Solubilizing Bacteria
103(1)
15.7 Isolation of Probiotic Bacteria
104(1)
15.8 Isolation of Bacteria from Spoiled Foods
105(1)
15.9 Isolation of Beta Lactamase Producing Bacteria
105(1)
15.10 Isolation of Polyhydroxyalkanoates (PHA) Producing Bacteria
106(1)
15.11 Isolation of Endophytic Bacteria
107(2)
15.12 Bacterial Examination of Water
109(3)
15.13 Phenol Coefficient Test
112(1)
15.14 Heavy Metals Sensitivity Test
113(1)
15.15 Growth Kinetics of Bacteria in the Presence of Antimicrobials
114(1)
15.16 Cellular Morphology Changes in Bacteria
114(2)
15.17 Dye Degradation by Bacteria
116(1)
15.18 Immobilization of Bacterial Cells
117(3)
15.19 Bacterial Mutation
120(1)
15.20 Bacterial Biomass Estimation
121(1)
15.21 Determination of Thermal Death Point and Thermal Death Temperature
121(1)
15.22 Screening of Bacterial Cell Free Supernatant for Compound Isolation
122(1)
15.23 Extraction of Bacteriocin
123(1)
15.24 Screening for Biosurfactant Production
123(2)
16 Bacterial Genetics
125(9)
16.1 Transformation
125(1)
16.2 Conjugation
126(1)
16.3 Transduction
127(1)
16.4 Isolation of Genomic DNA from Bacteria
128(2)
16.5 Isolation of Plasmid DNA from Bacteria
130(2)
16.6 Isolation of Total RNA from Bacterial Cells
132(2)
17 Nanotechnology in Bacteriology
134(6)
17.1 Silver Nanoparticle Synthesis
134(1)
17.2 Antibacterial Activity of Silver Nanoparticle
135(1)
17.3 Gold Nanoparticle Synthesis
136(1)
17.4 Biosynthesis of Titanium Dioxide Nanoparticle
137(2)
17.5 Biosynthesis of Lead Sulphide Nanoparticle
139(1)
18 Fumigation Procedure
140(1)
Appendices
141(29)
A List of Selected Gram (+) & Gram (-) Bacteria
141(1)
B Types of Media
141(1)
C Bacteriology Laboratory---Safety Rules and Regulations
142(2)
D Media for Bacteriology
144(18)
E Stains & Indicators for Bacteriology
162(1)
F Antibiotics Susceptibility Test Discs
163(1)
G Calculations
163(3)
H Terminology at a Glance
166(4)
Quotations 170(1)
References 171(3)
Index 174