This book acts as a clinical manual for the diagnostician who cannot turn to reference books when the morphology or immunophenotype are atypical. This volume presents a logical practical approach to the diagnosis of blood disorders, both neoplastic and reactive, and other diagnostic applications of flow cytometry in non-neoplastic haematology diagnosis. Illustrations are provided throughout with worked examples.
Recenzijas
I would particularly recommend this book to haematology trainees, as an introduction to, but not as a replacement for, a period spent in an immunophenotyping laboratory. (British Journal of Haematology, 14 November 2014) There is no doubt that this book will find its way firmly onto the bookshelf in every laboratory, and into the work bags of many trainees. (Haem Trainee, 1 January 2014) This is a concise and very practical guide to the use of flow cytometry in hematological and hematopathological diagnosis. While not as detailed in immunophenotyping intricacies as other books of its kind, it shines with its practical approach and expert guidance applied to current clinical practice. (Doody s, 19 July 2013)
Preface, vii Acknowledgements, viii 1 Introduction, 1 References, 2
2 Principles of Flow Cytometry, 3 Introduction, 3 Sample preparation, 3
The flow cytometer, 4 The fluidic system, 4 The optical system, 5 The
electronic system, 9 Data display, 9 Acknowledgements, 18 References, 19
3 Limitations, 20 Introduction, 20 Clinical context issues, 20 Sampling
issues, 20 Technical issues, 25 Interpretation issues, 28 Conclusion, 29
References, 29 4 Normal Blood and Bone Marrow Populations, 31 Normal stem
and precursor cell populations, 31 References, 41 5 Acute Leukaemia, 43
Introduction, 43 Identification of neoplastic precursor cells, 45 Lineage
assignment of acute leukaemia, 52 Acute myeloid leukaemia, 54 Precursor
lymphoid neoplasms (acute lymphoblastic leukaemia/lymphoma), 77 Examination
of cerebro-spinal fluid and serous effusions, 88 Identification of genetic
abnormalities by flow, 92 Conclusion, 93 References, 93 6 Chronic Lymphoid
Leukaemias and Exfoliating Lymphoma, 100 Normal peripheral blood lymphoid
populations, 100 Identification of clonal lymphoid populations, 101
Identification of clonal B-cell disorders, 101 CD5 positive B-cell
lymphoproliferative disorders, 102 CD10 positive B-cell lymphoproliferative
disorders, 116 B-cell disorders identified using an extended B-cell panel,
121 Identification of clonal T-cell disorders, 125 CD4 positive T-cell
disorders, 128 CD8 positive T-cell disorders, 136 CD4/CD8 positive T-cell
disorders, 141 CD4/8 negative disorders, 141 Flow cytometric assessment of
serous effusions, 146 Flow cytometric assessment of cerebrospinal fluid, 146
Summary, 149 References, 149 7 Myelodysplastic Syndromes and
Myeloproliferative Neoplasms, 152 Introduction, 152 Myelodysplastic
syndromes, 152 Myelodysplastic/myeloproliferative neoplasms, 163 Chronic
myeloid leukaemia, 165 The BCR-ABL negative myeloproliferative neoplasms,
166 Mastocytosis, 167 Summary, 170 References, 170 8 Disorders of Plasma
Cells, 173 Plasma cell disorders, 173 Current diagnostic criteria, 173
Plasma cell morphology, 175 The applications of immunophenotyping to plasma
cell disorders, 178 References, 183 9 Minimal Residual Disease, 184
Introduction, 184 Minimal residual disease analysis in the management of
acute lymphoblastic leukaemia, 187 Conclusions, 199 Acknowledgements, 200
References, 200 10 Red Cells, Leucocytes and Platelets, 202 Paroxysmal
nocturnal haemoglobinuria, 202 Red cell membrane disorders, 208 Foetal
maternal haemorrhage, 211 Lymphocyte subset analysis and immunodeficiency,
214 Haemopoietic stem cell enumeration, 214 Granulocyte disorders, 215
Platelet disorders, 219 References, 221 11 Reactive and Non-neoplastic
Phenomena, 225 Peripheral blood, 225 Bone marrow, 231 Pleural and
pericardial fluids, 236 Bronchoalveolar lavage specimens, 238 Cerebrospinal
fluid, 238 References, 240 Index, 243
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