Atjaunināt sīkdatņu piekrišanu

Proximity-Dependent Protein Biotinylation: Methods and Protocols [Hardback]

Edited by
  • Formāts: Hardback, 326 pages, height x width: 254x178 mm, 48 Illustrations, color; 7 Illustrations, black and white; XI, 326 p. 55 illus., 48 illus. in color., 1 Hardback
  • Sērija : Methods in Molecular Biology 2953
  • Izdošanas datums: 11-Aug-2025
  • Izdevniecība: Springer-Verlag New York Inc.
  • ISBN-10: 1071646931
  • ISBN-13: 9781071646939
Citas grāmatas par šo tēmu:
  • Hardback
  • Cena: 162,93 €*
  • * ši ir gala cena, t.i., netiek piemērotas nekādas papildus atlaides
  • Standarta cena: 191,69 €
  • Ietaupiet 15%
  • Grāmatu piegādes laiks ir 3-4 nedēļas, ja grāmata ir uz vietas izdevniecības noliktavā. Ja izdevējam nepieciešams publicēt jaunu tirāžu, grāmatas piegāde var aizkavēties.
  • Daudzums:
  • Ielikt grozā
  • Piegādes laiks - 4-6 nedēļas
  • Pievienot vēlmju sarakstam
Proximity-Dependent Protein Biotinylation: Methods and ProtocolsPalielināt
  • Formāts: Hardback, 326 pages, height x width: 254x178 mm, 48 Illustrations, color; 7 Illustrations, black and white; XI, 326 p. 55 illus., 48 illus. in color., 1 Hardback
  • Sērija : Methods in Molecular Biology 2953
  • Izdošanas datums: 11-Aug-2025
  • Izdevniecība: Springer-Verlag New York Inc.
  • ISBN-10: 1071646931
  • ISBN-13: 9781071646939
Citas grāmatas par šo tēmu:
Permanent link: https://www.kriso.lv/db/9781071646939.html

This volume explores the cutting-edge of proximity-dependent protein biotinylation approaches, focusing on innovative biotin ligase (BioID) and peroxidase-based methods, like APEX. Spanning a diverse array of biological systems, including bacteria, zebrafish, plants, and mammals, this collection showcases methods that aid in the identification of protein interactions, localization, membrane topology, trafficking, and secretion dynamics. Additionally, the book features applications at the host/microbe interface and for the use of tissue-specific biotinylation for studying interorgan communication, as well as in silico validation methods. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, detailed lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and expert tips for troubleshooting and avoiding known pitfalls.

 

Authoritative and practical, Proximity-Dependent Protein Biotinylation: Methods and Protocols serves as an essential guide for researchers advancing the study of proxeome biology.

Cross-Species Proximity-Dependent Protein Biotinylation: A Standardized
Approach for Mapping Proxeomes.- BioID in Bacteria: Selection of a Suitable
Biotin Ligase for Proxeome Mapping.- Uncovering Transient Protein
Interactions in the Yersinia enterocolitica Type III Secretion System Using
MiniTurbo-Mediated Proximity Labeling.- Exploring Bacterial Surface Proteome
Dynamics During Infection Using Proximity Labeling.- Identification of In
Vivo Protein Networks Using MiniTurbo Proximity Labeling in Bacteria.-
Unraveling N-Terminal Proteoform Interactomes via Multiplexed Recombineering
in Salmonella.- Probing Pairwise Protein-Protein Interactions with the
Bacterial POLAR Assay.- Detection and Quantification of Biotinylated Proteins
for TurboID-Based Proximity Labeling Mass Spectrometry in Arabidopsis.-
Detection and Quantification of Biotinylated Sites for TurboID-Based
Proximity Labeling Mass Spectrometry in Arabidopsis.- Coupling Affinity
Purification with Proximity Labeling in Arabidopsis: The APEAL Approach.-
Characterization of the Protein Interactome of Membrane-Bound Transcription
Factors Using TurboID-Based Proximity Labeling In Planta.- Identification of
Cytosol-Facing Organelle Membrane-Proximal Proteins via Proximity-Dependent
Biotinylation.- BioID2-Based Proximity Labeling in Adult Zebrafish.-
On-Demand Proximity Labeling Using Light-Activated BioID.- Tissue-Specific
Biotinylation for Interorgan Communication.- Spatiotemporally-Resolved
Profiling of Protein Movement by TransitID.- An Integrated High-Throughput
Proteocistromic Framework for Mapping the Interactome and Targetome of Human
Transcription Factors.- Step-by-Step Application of Proximity-Dependent
Biotinylation Tools for Identification of Astrocyte and Neuron Subproteomes
In Vivo Across the Whole Brain.- Antibody-Mediated Protein A-APEX2 Labeling
(AMAPEX) for Proximity Proteome Exploration.- A Ready-to-Use Data Analysis
Pipeline for BioID Experiments Using Data-Dependent or Data-Independent
Acquisition Mass Spectrometry.