This book provides a broad introduction to all major aspects of quantum dot properties including fluorescence, electrochemical, photochemical and electroluminescence. Such properties have been produced for applications in biosensing, cell tracking, in vivo animal imaging and so on. It focuses on their special applications in DNA biosensing and provides readers with detailed information on the preparation and functionalization of quantum dots and the fabrication of DNA biosensors, using examples to show how these properties can be used in DNA biosensor design and the advantages of quantum dots in DNA biosensing. Further new emerging quantum dots such as metal nanoclusters and graphene dots and their applications in DNA biosensing have also been included.
Introduction.- Quantum Dots.- QDs-Fluorescence Based Biosensing.- QDs-ECL based Biosensing.- QDs-Electrochemical and Photoelectrochemical Biosensing.
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1 | (8) |
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1.1 Overview of DNA Biosensing |
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1 | (1) |
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1.2 Overview of Quantum Dots |
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2 | (7) |
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3 | (1) |
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1.2.2 Electrochemiluminescence Property |
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4 | (1) |
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1.2.3 Electrochemical and Photoelectrochemical Property |
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4 | (1) |
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5 | (4) |
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9 | (16) |
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2.1 Traditional Quantum Dots |
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9 | (1) |
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2.2 New Emerging Quantum Dots |
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10 | (3) |
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11 | (1) |
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12 | (1) |
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13 | (1) |
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2.3 Preparation and Functionalization |
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13 | (12) |
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2.3.1 Cadmium-Based Quantum Dots |
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13 | (2) |
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2.3.2 Cadmium-Free Quantum Dots |
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15 | (2) |
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17 | (2) |
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2.3.4 Quantum Dot Bioconjugation |
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19 | (1) |
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19 | (6) |
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3 Quantum Dot-Fluorescence-Based Biosensing |
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25 | (28) |
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25 | (10) |
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3.1.1 Main Types for DNA Detection |
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25 | (7) |
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3.1.2 Multiplex DNA Detection |
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32 | (3) |
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3.2 QDs for RNA Detection |
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35 | (10) |
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3.2.1 Direct Fluorescence Labeling |
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36 | (3) |
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3.2.2 Foster (or Fluorescence) Resonance Energy Transfer System |
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39 | (2) |
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3.2.3 Sensing Based on DNA-Scaffolded Metal Nanoclusters |
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41 | (3) |
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3.2.4 Sensing Based on Fluorescence In Situ Hybridization |
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44 | (1) |
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3.3 QDs for DNA Microarrays |
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45 | (8) |
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48 | (5) |
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4 Quantum Dot-Electrochemiluminescence-Based Biosensing |
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53 | (18) |
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54 | (6) |
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4.1.1 ECL of Semiconductor QDs |
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55 | (3) |
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58 | (2) |
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4.2 QDs ECL for DNA Biosensing |
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60 | (11) |
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4.2.1 QDs ECL for DNA Analysis |
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60 | (2) |
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4.2.2 QDs ECL for Aptasensor Analysis |
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62 | (5) |
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67 | (4) |
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5 Quantum Dot-Electrochemical and Photoelectrochemical Biosensing |
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71 | |
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5.1 QDs as Electrochemical Labels |
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71 | (8) |
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5.1.1 The Electrochemical Behavior of QDs |
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71 | (4) |
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5.1.2 The Electrochemical DNA Analysis of QDs |
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75 | (2) |
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5.1.3 The Electrochemical Aptamer Analysis of QDs |
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77 | (2) |
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5.2 QDs for Photoelectrochemical Analysis |
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79 | |
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89 | |
Jun-Jie Zhu received his B.S. degree in Chemistry from Nanjing University in 1984 and his Ph.D. degree from the same university in 1993. From 1998 to 1999, he was a postdoctoral research fellow at Bar Ilan University, Israel. He is currently serving as a full professor at Nanjing University, where he was honored by National Natural Science Funds for Distinguished Young Scholars of China in 2003. Professor Zhu mainly works in nano-bioanalytical chemistry, including the synthesis and characterization of functionalized nanomaterials, bioelectrochemistry and nanoelectrochemistry, optical analysis of nanomaterials, regulation and manipulation on microfluidic chips using nanomaterials and the bio-application of nanomaterials.
Jing-Jing Li obtained her B. Sc. in 2005 and M. Sc. in 2008 from Suzhou University. In 2012, she obtained her Ph. D. from Nanjing University under the supervision of Prof. Jun-Jie Zhu and Jian-Rong Zhang. She is currently a research associate at Xuzhou Medical College. Her research interest during her doctoral studies mainly focused on aptamer biosensors and novel functional fluorescent molecular probes for cell imaging.
Hai-Ping Huang received his B. Sc. in 2003 from Southwest University and his M. Sc. in 2006 from Changchun Institute of Applied Chemistry, Chinese Academy of Sciences. In 2009 he completed his Ph. D. at Nanjing University under the supervision of Prof. Jun-Jie Zhu. Currently he is an associate professor at Jiangxi University of Science and Technology. His general research interests include the synthesis, characterization and bio-related applications of novel nanomaterials.
Fang-Fang Cheng obtained her Bachelor degree in 2008 from Yangzhou University. She is currently a senior PhD candidate at Nanjing University, working under the supervision of Prof. Jun-Jie Zhu. Her research interest is mainly in the development of drug delivery systems as well as the optical and electrochemical detection of cancer markers.
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