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Reactive Oxygen Species: Methods and Protocols 2021 ed. [Hardback]

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  • Formāts: Hardback, 233 pages, height x width: 254x178 mm, weight: 671 g, 42 Illustrations, color; 21 Illustrations, black and white; XI, 233 p. 63 illus., 42 illus. in color., 1 Hardback
  • Sērija : Methods in Molecular Biology 2202
  • Izdošanas datums: 29-Aug-2020
  • Izdevniecība: Springer-Verlag New York Inc.
  • ISBN-10: 1071608959
  • ISBN-13: 9781071608951
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  • Hardback
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  • Formāts: Hardback, 233 pages, height x width: 254x178 mm, weight: 671 g, 42 Illustrations, color; 21 Illustrations, black and white; XI, 233 p. 63 illus., 42 illus. in color., 1 Hardback
  • Sērija : Methods in Molecular Biology 2202
  • Izdošanas datums: 29-Aug-2020
  • Izdevniecība: Springer-Verlag New York Inc.
  • ISBN-10: 1071608959
  • ISBN-13: 9781071608951
Citas grāmatas par šo tēmu:
This volume provides a varied selection of current methods and molecular probes used to generate, image and quantify ROS production and lipid peroxidation. Chapters guide readers through methods and protocols on in silico modelling of ROS dynamics in biological systems and a breaking proposal. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.





 





Authoritative and cutting-edge,  Reactive Oxygen Species: Methods and Protocols aims to ensure successful results in the further study of this vital field.
Introduction to in silico Modeling to Study ROS Dynamics.- Assessment of
ROS Production in Mitochondria of Live Cells.- Measurement of Superoxide
Production in Acute Hypoxia by Fixed-Cell Microscopy.- A Photodynamic Tool to
Promote a Sustained, ROS-Dependent Growth of Human Hair Follicles in ex vivo
Culture.- Singlet Oxygen and Protochlorophyllide Detection in Arabidopsis
thaliana.- Characterization of oxidative lipidomics and autophagy induction
in Chlamydomonas reinhardtii under abiotic stress.- Fluorescence Detection of
Increased Reactive Oxygen Species Levels in Saccharomyces cerevisiae at the
Diauxic Shift.- Flow Cytometry and Confocal Microscopy for ROS Evaluation in
Fish and Human Spermatozoa.- Measurement of Reactive Oxygen Species In Semen
Samples Using Chemiluminescence.- Methods to Unravel Pathways of Reactive
Oxygen Species in the Photodynamic Inactivation of Bacteria.- Revealing ROS
Production by Antibiotics and Photosensitizers in Biofilms. A Fluorescence
Microscopy Approach.- EPR Spectroscopy as a Method for ROS Quantification in
Skin.- Detection of Superoxide Radical in Adherent Living Cells by Electron
Paramagnetic Resonance (EPR) Spectroscopy Using Cyclic Nitrones.- Singlet
Oxygen Quantum Yield Determination Using Chemical Acceptors.- A General
Method to Establish the Relative Efficiency of Different Sonosensitizers to
Generate ROS for SDT.- Lipid Peroxidation Assay using BODIPY-phenylbutadiene
Probes:A Methodological Overview.- Use of Fluorescent Probes to Detect ROS in
Photodynamic Therapy.