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E-grāmata: Recombinant Glycoproteins: Methods and Protocols

  • Formāts: EPUB+DRM
  • Sērija : Methods in Molecular Biology 2762
  • Izdošanas datums: 05-Feb-2024
  • Izdevniecība: Springer-Verlag New York Inc.
  • Valoda: eng
  • ISBN-13: 9781071636664
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  • Formāts: EPUB+DRM
  • Sērija : Methods in Molecular Biology 2762
  • Izdošanas datums: 05-Feb-2024
  • Izdevniecība: Springer-Verlag New York Inc.
  • Valoda: eng
  • ISBN-13: 9781071636664
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This detailed volume explores the production and purification of glycoproteins for therapeutic use or basic research, as well as the necessary in-depth understanding of cellular and acellular systems available for these purposes, their advantages and disadvantages, and considerations for choosing the most appropriate system for the desired application. Beginning with chapters on viral-encoded glycoproteins and those found in other pathogens, the book continues by examining the production of mammalian glycoproteins, the analysis of glycoprotein content, cell-free synthesis of glycoproteins and considerations for production and purification of glycoproteins. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to the respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.



Authoritative and practical, Recombinant Glycoproteins: Methods and Protocols provides a wide range of guidelines for studying these vitally important proteins.



Chapter Solubilization of Oligomeric Cell-Free Synthesized Proteins Using SMA Copolymers is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.
Production and Purification of Hantavirus Glycoproteins in Drosophila
melanogaster S2 Cells.- Production and Purification of Filovirus
Glycoproteins.- Modification of N-Linked Glycan Sites in Viral
Glycoproteins.- Production of Influenza Virus Glycoproteins Using Insect
Cells.- SARS-CoV-2 S-Protein Ace2 Binding Analysis Using Surface Plasmon
Resonance.- A Biosensor Assay Based on Coiled-Coil-Mediated Human ACE2
Receptor Capture for the Analysis of Its Interactions with the SARS-CoV-2
Receptor Binding Domain.- Production and Purification of Plasmodium
Circumsporozoite Protein in Lactococcus lactis.- Analysis of Caenorhabditis
Protein Glycosylation.- Use of Reductive Amination to Produce Capsular
Polysaccharide-Based Glycoconjugates.- Overexpression and Purification of
Mitogenic and Metabolic Fibroblast Growth Factors.- Production and
Purification of Antibodies in Chinese Hamster Ovary Cells.- Mammalian Antigen
Display for Pandemic Countermeasures.- Analysisof Native and Permethylated
N-Glycan Isomers Using MGC-LC-MS Techniques.- Targeted Glycoproteomics
Analysis Using MRM/PRM Approaches.- Targeted Analysis of Permethylated
N-Glycans Using MRM/PRM Approaches.- Hydrophilic Interaction Liquid
Chromatography (HILIC) Enrichment of Glycopeptides Using PolyHYDROXYETHYL
A.- O-Glycoproteomics Sample Preparation and Analysis Using NanoHPLC and
Tandem MS.- Solubilization of Oligomeric Cell-Free Synthesized Proteins Using
SMA Copolymers.- Cell-Free Systems for the Production of Glycoproteins.-
Considerations for Glycoprotein Production.