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E-grāmata: Regulators and Effectors of Small GTPases, Part D: Rho Family

Editor-in-chief (California Institute of Technology, Division of Biology, Pasadena, U.S.A.), Volume editor (Lineberger Comprehensive Cancer Center, University of North Caro), Editor-in-chief (The Salk Institute, La Jolla, CA, USA), Volume editor , Volume editor (The Scripps Research Institute, La Jolla, CA, USA)
  • Formāts: PDF+DRM
  • Sērija : Methods in Enzymology
  • Izdošanas datums: 11-Oct-2000
  • Izdevniecība: Academic Press Inc
  • Valoda: eng
  • ISBN-13: 9780080496801
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Regulators and Effectors of Small GTPases, Part D: Rho FamilyPalielināt
  • Formāts: PDF+DRM
  • Sērija : Methods in Enzymology
  • Izdošanas datums: 11-Oct-2000
  • Izdevniecība: Academic Press Inc
  • Valoda: eng
  • ISBN-13: 9780080496801
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This volume and its companions (Volumes 255, 256, 257, and the forthcoming 329) cover all biochemical and biological assays currently in use for analyzing the role of small GTPases in these aspects of cell biology at the molecular level.
The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today--truly an essential publication for researchers in all fields of life sciences.

Recenzijas

Praise for the Series "The Methods in Enzymology series represents the gold-standard." --NEUROSCIENCE "Incomparably useful." --ANALYTICAL BIOCHEMISTRY "It is a true 'methods' series, including almost every detail from basic theory to sources of equipment and reagents, with timely documentation provided on each page." --BIO/TECHNOLOGY "The series has been following the growing, changing and creation of new areas of science. It should be on the shelves of all libraries in the world as a whole collection." --CHEMISTRY IN INDUSTRY "The appearance of another volume in that excellent series, Methods in Enzymology, is always a cause for appreciation for those who wish to successfully carry out a particular technique or prepare an enzyme or metabolic intermediate without the tiresome prospect of searching through unfamiliar literature and perhaps selecting an unproven method which is not easily reproduced." --AMERICAN SOCIETY OF MICROBIOLOGY NEWS "If we had some way to find the work most often consulted in the laboratory, it could well be Colowick and Kaplan's multi-volume series Methods in Enzymology...a great work." --ENZYMOLOGIA "A series that has established itself as a definitive reference for biochemists." --JOURNAL OF CHROMATOGRAPHY

Contributors to Volume 325 ix Preface xv Volumes in Series xvii Section I. Purification, Posttranslational Modification, and in Vitro Regulation Purification and Biochemical Characterization of TC10 3(12) Cheryl L. Neudauer Ian G. Macara Expression and Purification of Rho/RhoGDI Complexes 15(10) Paul W. Read Robert K. Nakamoto Bacterial Expressed DH and DH/PH Domains 25(13) Kent L. Rossman Sharon L. Campbell Biochemical Analysis of Regulation of Vav, a Guanine-Nucleotide Exchange Factor for Rho Family of GTPases 38(13) Raymond Mosteller Jaewon Han Balaka Das Daniel Broek Activation of Rac1 by Human Tiam1 51(10) Gideon Bollag Anne M. Crompton Danielle Peverly-Mitchell Gaston G. M. Habets Marc Symons Activation of Rho GEF Activity by G13 61(10) Matthew J. Hart William Roscoe Gideon Bollag Purification and Characterization of Guanine Nucleotide Dissociation Stimulator Protein 71(11) Jon P. Hutchinson Katrin Rittinger John F. Eccleston Purification and Biochemical Activity of Salmonella Exchange Factor SopE 82(9) Andrea Friebel Wolf-Dietrich Hardt Stimulation of Rho GDI Release by ERM Proteins 91(10) Akiko Mammoto Kazuo Takahashi Takuya Sasaki Yoshimi Takai Expression and Activity of Human Prenylcysteine-Directed Carboxyl Methyltransferase 101(13) Edwin Choy Mark Philips Purification and Evaluation of Large Clostridial Cytotoxins That Inhibit Small GTPases of Rho and Ras Subfamilies 114(11) Michael Moos Christoph von Eichel-Streiber Rho GTPase-Activating Toxins: Cytotoxic Necrotizing Factors and Dermonecrotic Toxin 125(14) Gudula Schmidt Klaus Aktories Section II. Purification and Activity of GTPase Targets Measurement of GTPase Effector Affinities 139(10) Danny Manor Purification and in Vitro Activity of Rho-Associated Kinase 149(6) Mutsuki Amano Yuko Fukata Hiroaki Shimokawa Kozo Kaibuchi Purification and in Vitro Activities of p21-Activated Kinases 155(12) Charles C. King Abina M. Reilly Ulla G. Knaus Stimulation of Phospholipase C-2 by Rho GTPases 167(10) Daria Illenberger Ilona Stephan Peter Gierschik Frieder Schwald Regulation of Phospholipase D1 Activity by Rho GTPases 177(13) Michael A. Frohman Yasunori Kanaho Yue Zhang Andrew J. Morris In Vitro Interaction of Phosphoinositide-4-phosphate 5-Kinases with Rac 190(10) Kimberley Tolias Christopher L. Carpenter Activity and Regulation of p35/Cdk5 Kinase Complex 200(14) Margareta Nikolic Li-Huei Tsai Actin Assembly Mediated by Arp2/3 Complex and WASP Family Proteins 214(23) R. Dyche Mullins Laura M. Machesky In Vitro Actin Polymerization Using Polymorpho-nuclear Leukocyte Extracts 237(20) Sally H. Zigmond Section III. Analysis of Rho GTPase Function Determination of GTP Loading on Rac and Cdc42 in Platelets and Fibroblasts 257(7) Anser C. Azim Kurt L. Barkalow John H. Hartwig Determination of GTP Loading on Rho 264(9) Xiang-Dong Ren Martin Alexander Schwartz Use and Properties of ROCK-Specific Inhibitor Y-27632 273(12) Shuh Narumiya Toshimasa Ishizaki Masayoshi Uehata Inducible Membrane Recruitment of Small GTP-Binding Proteins by Rapamycin-Based System in Living Cells 285(10) Flavia Castellano Philippe Chavrier Expression of Rho GTPases Using Retroviral Vectors 295(8) Frits Michiels Rob A. van der Kammen Lennert Janssen Garry Nolan John G. Collard Expression of Rho GTPases Using Adenovirus Vectors 303(12) Daniel M. Sullivan Toren Finkel In Vivo Activity of Wild-Type and Mutant PAKs 315(12) Charles C. King Luraynne C. Sanders Gary M. Bokoch Single Cell Assays for Rac Activity 327(7) Laura J. Taylor Amy B. Walsh Patrick Hearing Dafna Bar-Sagi Effect of Rho GTPases on Na-H Exchanger in Mammalian Cells 334(15) Sheryl P. Denker Weihong Yan Diane L. Barber Actin Filament Assembly in Permeabilized Platelets 349(7) John H. Hartwig Kurt L. Barkalow Rho GTPases: Secretion and Actin Dynamics in Permeabilized Mast Cells 356(13) Mark R. Holt Anna Koffer Isolation and in Vitro Contraction of Stress Fibers 369(12) Kazuo Katoh Yumiko Kano Keigi Fujiwara Inhibition of Rho GTPases Using Protein Geranylgeranyltransferase I Inhibitors 381(8) Said M. Sebti Andrew D. Hamilton Imaging Spatiotemporal Dynamics of Rac Activation in Vivo with FLAIR 389(14) Chester E. Chamberlain Vadim S. Kraynov Klaus M. Hahn Section IV. Biological Assays of Rho GTPase Function Rho-like GTPases in Tumor Cell Invasion 403(12) Eva E. Evers Rob A. van der Kammen Jean P. ten Klooster John G. Collard Mouse Embryo Fibroblasts: A Genetic Model System for Studying Rho-and Ras-Dependent Cell Cycle Progression 415(10) Giovanna M. DAbaco Michael F. Olson Analyses of Transforming Activity of Rho Family Activators 425(16) Patricia A. Solski Karon Abe Channing J. Der Rho GTPases and Cell Migration-Fibroblast Wound Healing 441(8) Catherine D. Nobes Rho GTPases and Cell Migration: Measurement of Macrophage Chemotaxis 449(13) Gareth E. Jones Anne J. Ridley Daniel Zicha Rho GTPases and Macrophage Phagocytosis 462(11) Jayesh C. Patel Alan Hall Emmanuelle Caron Rho GTPases and Axonal Growth Cone Collapse 473(9) Alyson E. Fournier Robert G. Kalb Stephen M. Stritimatter Study of in Situ Function of Cytoskeletal Proteins in Lamellipodia and Filopodia Using Microscale Chromophore-Assisted Laser Inactivation 482(14) Eric V. Wong Daniel G. Jay Modulation of Actin Cytoskeleton by Salmonella GTPase Activating Protein SptP 496(9) Jorge E. Galan Yixin Fu Author Index 505(26) Subject Index 531
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