Small GTPases play a key role in many aspects of contemporary cell biology: control of cell growth and differentiation; regulation of cell adhesion and cell movement; the organization of the actin cytoskeleton; and the regulation of intracellular vesicular transport.
This volume and its companions (Volumes 255, 256, 257, and the forthcoming 325) cover all biochemical and biological assays currently in use for analyzing the role of small GTPases in these aspects of cell biology at the molecular level.
Contributors to Volume 329 xi Preface xvii Volumes in Series xix Section I. Rab GTPases Structural Basis for Rab Function: An Overview 3(3) Bryan D. Moyer William E. Balch Expression of Wild-Type and Mutant Green Fluorescent Protein--Rab1 for Fluorescence Microscopy Analysis 6(8) Bryan D. Moyer Jeanne Matteson William E. Balch Fluorescence Methods for Monitoring Interactions of Rab Proteins with Nucleotides, Rab Escort Protein, and Geranylgeranyltransferase 14(17) Kirill Alexandrov Axel J. Scheidig Roger S. Goody Prenylation of Rab Proteins in Vitro by Geranylgeranyltransferases 31(8) Jean H. Overmeyer William A. Maltese Antibody and Oligonucleotide Probes to Distinguish Intracellular Expression and Localization Patterns of Rab GDP-Dissociation Inhibitor Isoforms 39(11) Assia Shisheva Expression, Purification, and Biochemical Properties of Ypt/Rab GTPase-Activating Proteins of Gyp Family 50(9) Elke Will Stefan Albert Dieter Gallwitz Purification and Properties of Rab3 GDP/GTP Exchange Protein 59(8) Hiroyuki Nakanishi Yoshimi Takai Purification and Properties of Rab3 GTPase-Activating Protein 67(8) Fumiko Nagano Takuya Sasaki Yoshimi Takai Rabphilin-3: A Target Molecule for Rab3 Small G Proteins 75(8) Hiromichi Shirataki Takuya Sasaki Yoshimi Takai Doc2α as Modulator of Ca2+-Dependent Exocytosis 83(8) Satoshi Orita Takuya Sasaki Yoshimi Takai Purification and Properties of Sec4p GTPase-Activating Protein 91(9) Li-Lin Du Peter Novick Purification and Characterization of Yeast Exocyst Complex 100(11) Daniel R. TerBush Wei Guo Steven Dunkelbarger Peter Novick Expression and Properties of Rab4 and Its Effector Rabaptin-4 in Endocytic Recycling 111(9) Peter Van Der Sluijs Karin Mohrmann Magda Deneka Mandy Jongeneelen Purification of EEA1 from Bovine Brain Cytosol Using Rab5 Affinity Chromatography and Activity Assays 120(12) Savvas Christoforidis Marino Zerial Expression, Purification, and Characterization of Rab5 Effector Complex, Rabaptin-5/Rabex-5 132(13) Roger Lippe Hisanori Horiuchi Anja Runge Marino Zerial Measurement of Rab5 Protein Kinase B/akt and Regulation of Ras-Activated Endocytosis 145(12) Manuel A. Barbieri A. Gumusboga Richard L. Roberts Philip D. Stahl Expression, Purification, and Biochemical Properties of Rabkinesin-6 Domains and Their Interactions with Rab6A 157(8) Arnaud Echard Ahmed El Marjou Bruno Goud Expression and Functional Analyses of Rab8 and Rab11a in Exocytic Transport from trans-Golgi Network 165(10) Wei Chen Angela Wandinger-Ness Expression and Properties of Rab7 in Endosome Function 175(13) Yan Feng Barry Press Wei Chen Jay Zimmerman Angela Wandinger-Ness Expression, Purification, and Properties of Rab8 Function in Actin Cortical Skeleton Organization and Polarized Transport 188(9) John Peranen Johanna Furuhjelm Properties of Rab13 Interaction with Rod cGMP Phosphodiesterase δ Subunit 197(13) Anne-Marie Marzesco Thierry Galli Daniel Louvard Ahmed Zahraoui Subcellular Localization of Rab17 by Cryo-Immunogold Electron Microscopy in Epithelial Cells Grown on Polycarbonate Filters 210(15) Peter J. Peters Walter Hunziker Expression and Properties of Rab25 in Polarized Madin--Darby Canine Kidney Cells 225(9) James R. Goldenring Lorraine M. Aron Lynne A. Lapierre Jennifer Navarre James E. Casanova Purification of TRAPP from Saccharomyces cerevisiae and Identification of Its Mammalian Counterpart 234(11) Michael Sacher Susan Ferro-Novick Section II. ADP-Ribosylation Factor (ARF) GTPases Structural and Functional Organization of ADP-Ribosylation Factor (ARF) Proteins 245(2) Sophie Beraud-Dufour William E. Balch Expression and Properties of ADP-Ribosylation Factor (ARF6) in Endocytic Pathway 247(9) Julie G. Donaldson Harish Radhakrishna Expression and Analysis of ARNO and ARNO Mutants and Their Effects on ADP-Ribosylation Factor (AFR)-Mediated Actin Cytoskeletal Rearrangements 256(8) Lorraine C. Santy Scott R. Frank James E. Casanova Expression, Purification, and Measurements of Activity of ARNO1, a Guanine Nucleotide Exchange Factor for ADP-Ribosylation Factor 1 (ARF1) 264(8) Sophie Beraud-Dufour Sylviane Robineau Expression, Purification, and Biochemical Properties of EFA6, a Sec7 Domain-Containing Guanine Exchange Factor for ADP-Ribosylation Factor 6 (ARF6) 272(7) Philippe Chavrier Michel Franco Isolation and Properties of GRP1, an ADP-Ribosylation Factor (ARF)-Guanine Nucleotide Exchange Protein Regulated by Phosphatidylinositol 3,4,5-Trisphosphate 279(11) Jes K. Klarlund Michael P. Czech Functional Analysis of ADP-Ribosylation Factor (ARF) Guanine Nucleotide Exchange Factors Gea1p and Gea2p in Yeast 290(10) Anne Peyroche Catherine L. Jackson Isolation, Cloning, and Characterization of Brefeldin A-Inhibited Guanine Nucleotide-Exchange Protein for ADP-Ribosylation Factor 300(7) Gustavo Pacheco-Rodriguez Joel Moss Martha Vaughan Expression, Purification, and Properties of ADP-Ribosylation Factor (ARF) GAP1 307(10) Irit Huber Miriam Rotman Elah Pick Vardit Makler Lilah Rothem Edna Cukierman Dan Cassel Expression, Analysis, and Properties of Yeast ADP-Ribosylation Factor (ARF) GTPase Activating Proteins (GAPs) Gcs1 and Glo3 317(7) Pak Phi Poon Dan Cassel Irit Huber Richard A. Singer Gerald C. Johnston Purification and Properties of ARD1, an ADP-Ribosylation Factor (ARF)-Related Protein with GTPase-Activating Domain 324(11) Nicolas Vitale Joel Moss Martha Vaugflan Purification and Characterization of GIT Family of ADP-Ribosylation Factor (ARF) GTPase-Activating Proteins 335(8) Richard T. Premont Nicolas Vitale Assay and Purification of Phosphoinositide-Dependent ADP-Ribosylation Factor (ARF) GTPase-Activating Proteins 343(12) Paul A. Randazzo Koichi Miura Trevor R. Jackson Biological Properties and Measurement of Phospholipase D Activation by ADP-Ribosylation Factor (ARF) 355(17) Shamshad Cockcroft Kun Bi Nicholas T. Ktistakis Michael G. Roth Use of Aminoglycoside Antibiotics and Related Compounds to Study ADP-Ribosylation Factor (ARF)/Coatomer Function in Golgi Traffic 372(7) Rockford K. Draper Robert Tod Hudson Tonghuan Hu Adaptor Protein 1-Dependent Clathrin Coat Assembly on Synthetic Liposomes and Golgi Membranes 379(9) Yunxiang Zhu Matthew T. Drake Stuart Kornfeld Receptor-Dependent Formation of COPI-Coated Vesicles from Chemically Defined Donor Liposomes 388(17) Walter Nickel Felix T. Wieland ADP-Ribosylation Factor (ARF) as Regulator of Spectrin Assembly at Golgi Complex 405(12) Maria Antonietta De Matteis Jon S. Morrow Purification, Properties, and Analysis of yARL3 417(7) Fang-Jen S. Lee Chun-Fang Huang Preparation and Assay of Recombinant ADP-Ribosylation Factor-Like Protein-1 (ARL1) 424(7) Gustavo Pacheco-Rodriguez Joel Moss Martha Vaughan Section III. Sar GTPases Purification and Properties of Rat Liver Sec23-Sec24 Complex 431(7) Jacques T. Weissman Meir Aridor William E. Balch Purification of Functional Sec13p--Sec31p Complex, A Subunit of COPII Coat 438(9) William J. Belden Charles Barlowe Section IV. Dynamin GTPases Expression, Purification, and Functional Assays for Self-Association of Dynamin-1 447(10) Hanna Damke Amy B. Muhlberg Sanja Sever Steven Sholly Dale E. Warnock Sandra L. Schmid Analysis of Phosphoinositide Binding by Pleckstrin Homology Domain from Dynamin 457(11) Anthony Lee Mark A. Lemmon Mapping Dynamin Interdomain Interactions with Yeast Two-Hybrid and Glutathione S-Transferase Pulldown Experiments 468(10) Elena Smirnova Dixie-Lee Shurland Alexander M. Vander Bliek Interaction of Dynamin and Amphiphysin with Liposomes 478(8) Kohji Takei Vladimir I. Slepnev Pietro De Camilli Activation of Dynamin GTPase Activity by Phosphoinositides and SH3 Domain-Containing Proteins 486(13) Barbara Barylko Derk D. Binns Joseph P. Albanesi Section V. Septin GTPases Expression and Analysis of Properties of Septin CDCrel-1 in Exocytosis 499(12) Crestina L. Bettes Xiao-Rong Peng William S. Trimble Author Index 511(26) Subject Index 537
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