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Regulators and Effectors of Small GTPases, Part F: Ras Family I, Volume 332 [Hardback]

Volume editor (The Scripps Research Institute, La Jolla, CA, USA), Editor-in-chief (The Salk Institute, La Jolla, CA, USA), Volume editor , Editor-in-chief (California Institute of Technology, Division of Biology, Pasadena, U.S.A.), Volume editor (Lineberger Comprehensive Cancer Center, University of North Caro)
  • Formāts: Hardback, 492 pages, height x width: 229x152 mm, weight: 830 g
  • Sērija : Methods in Enzymology
  • Izdošanas datums: 17-Apr-2001
  • Izdevniecība: Academic Press Inc
  • ISBN-10: 0121822338
  • ISBN-13: 9780121822330
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Regulators and Effectors of Small GTPases, Part F: Ras Family I, Volume 332Palielināt
  • Formāts: Hardback, 492 pages, height x width: 229x152 mm, weight: 830 g
  • Sērija : Methods in Enzymology
  • Izdošanas datums: 17-Apr-2001
  • Izdevniecība: Academic Press Inc
  • ISBN-10: 0121822338
  • ISBN-13: 9780121822330
Citas grāmatas par šo tēmu:
Permanent link: https://www.kriso.lv/db/9780121822330.html
The critically acclaimed laboratory standard for more than 40 years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today, truly an essential publication for researchers in all fields of life sciences.
This volume and its companions (Volumes 255, 256, 257, and the forthcoming 325 and 329) cover all biochemical and biological assays currently in use for analyzing the role of small GTPases in these aspects of cell biology at the molecular level.

Recenzijas

"As a collection of methods, this work is exceptional for the level of detail included. ...This is a necessary addition to the library of anyone interested in the biology of small GTPases." Stephen A. Adam for DOODY PUBLICATION REVIEWS (2002)

Contributors to Volume 332 ix Preface xiii Volumes in Series xv Section
1. Protein Expression and Protein-Protein Interactions Mammalian Expression Vectors for Ras Family Proteins: Generation and Use of Expression Constructs to Analyze Ras Family Function 3(33) James J. Fiordalisi Ronald L. Johnson II Aylin S. Ulko Channing J. Der Adrienne D. Cox Protein Transduction: Delivery of Tat-GTPase Fusion Proteins into Mammalian Cells 36(14) Adamina Vocero-Akbani Meena A. Chellaiah Keith A. Hruska Steven F. Dowdy Green Fluorescent Protein-Tagged Ras Proteins for Intracellular Localization 50(14) Edwin Choy Mark Philips Targeting Proteins to Membranes, Using Signal Sequences for Lipid Modifications 64(13) John T. Stickney Michelle A. Booden Janice E. Buss Targeting Proteins to Specific Cellular Compartments to Optimize Physiological Activity 77(11) Garabet G. Toby Erica A. Golemis Mapping Protein-Protein Interactions with Alkaline Phosphatase Fusion Proteins 88(15) Montarop Yamabhai Brian K. Kay Assays of Human Postprenylation Processing Enzymes 103(12) Yun-Jung Choi Michael Niedbala Mark Lynch Marc Symons Gideon Bollag Anne K. North In Vivo Prenylation Analysis of Ras and Rho Proteins 115(12) Paul T. Kirschmeier David Whyte Oswald Wilson W. Robert Bishop Jin-Keon Pai Ras Interaction with RalGDS Effector Targets 127(12) Shinya Koyama Akira Kikuchi RAS Interaction with RINI Effector Target 139(12) Ying Wang John Colicelli Ras and Rapl Interaction with AF-6 Effector Target 151(20) Benjamin Boettner Christian Herrmann Linda Van Aelst Section II. Screening Analyses Analysis of Protein Kinase Specificity by Peptide Libraries and Prediction of in Vivo Substrates 171(12) Zhou Songyang Peptide Library Screening for Determination of SH2 or Phosphotyrosine-Binding Domain Sequences 183(12) Zhou Songyang Dan Liu Expression Cloning of Farnesylated Proteins 195(8) Douglas A. Andres Expression Cloning to Identify Monomeric GTP-Binding Proteins by GTP Overlay 203(8) Douglas A. Andres Retrovirus cDNA Expression Library Screening for Oncogenes 211(10) Gwendolyn M. Mahon Ian P. Whitehead Identification of Ras-Regulated Genes by Representational Difference Analysis 221(12) Janiel M. Shields Channing J. Der Scott Powers Differential Display Analysis of Gene Expression Altered by ras Oncogene 233(12) Hakryul Jo Yong-Jig Cho Hong Zhang Peng Liang cDNA Array Analyses of K-Ras-Induced Gene Transcription 245(15) Gaston G. Habets Marc Knepper Jaina Sumortin Yun-Jung Choi Takehiko Sasazuki Senji Shirasawa Gideon Bollag Ras Signaling Pathway for Analysis of Protein-Protein Interactions 260(10) Ami Aronheim Isolation of Effector-Selective Ras Mutants by Yeast Two-Hybrid Screening 270(7) Kiran J. Kaur Michael A. White Two-Hybrid Dual Bait System to Discriminate Specificity of Protein Interactions in Small GTPases 277(23) Ilya G. Serebriiskii Olga V. Mitina Jonathan Chernoff Erica A. Golemis Functional Proteomics Analysis of GTPase Signaling Networks 300(19) Gordon Alton Adrienne D. Cox L. Gerard Toussaint III John K. Westwick Section III. Analyses of Mitogen-Activated Protein Kinase Cascades Analyzing JNK and p38 Mitogen-Activated Protein Kinase Activity 319(18) Alan J. Whitmarsh Roger J. Davis Phospho-Specific Mitogen-Activated Protein Kinase Antibodies for ERK, JNK, and p38 Activation 337(6) Said A. Goueli Bruce W. Jarvis Immunostaining for Activated Extracellular Signal-Regulated Kinases in Cells and Tissues 343(10) Daniel G. Gioeli Maja Zecevic Michael J. Weber Dominant Negative Mutants of Mitogen-Activated Protein Kinase Pathway 353(15) M. Jane Arboleda Derek Eberwein Barbara Hibner John F. Lyons Scaffold Protein Regulation of Mitogen-Activated Protein Kinase Cascade 368(19) Andrew D. Catling Scott T. Eblen Hans J. Schaeffer Michael J. Weber Bacterial Expression of Activated Mitogen-Activated Protein Kinases 387(14) Julie L. Wilsbacher Melanie H. Cobb Steroid Receptor Fusion Proteins for Conditional Activation of Raf-MEK-ERK Signaling Pathway 401(16) Martin McMahon Pharmacologic Inhibitors of MKK1 and MKK2 417(15) Natalie G. Ahn Theresa Stines Nahreini Nicholas S. Tolwinski Katheryn A. Resing Analysis of Pharmacologic Inhibitors of Jun N-Terminal Kinases 432(21) Brion W. Murray Brydon L. Bennett Dennis T. Sasaki Author Index 453(24) Subject Index 477