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E-grāmata: Reprogramming the Genome: Applications of CRISPR-Cas in non-mammalian systems part A

Volume editor (Department of Biosciences, School of Science, Indrashil University, Rajpur, Mehsana, India)
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Reprogramming the Genome: Applications of CRISPR-Cas in Non-mammalian Systems, Part A presents a collation of chapters written by global, eminent scientists. CRISPR-Cas9 system is an RNA-mediated immune system of bacteria and archaea that protects from bacteriophage infections. It is one of the revolutionized technologies to uplift biology to the next stages. Chapters in this release include An Introduction and applications of CRISPR-Cas Systems, History, evolution and classification of CRISPR-Cas associated systems, CRISPR based bacterial genome editing and removal of pathogens, CRISPR based genome editing and removal of human viruses, CRISPR based development of RNA editing and diagnostic platform, and much more.

Additional sections cover Genome engineering in insects for control of vector borne diseases, Development of insect cell line using CRISPR technology, CRISPRing protozoan parasites to better understand the biology of diseases, CRISPR based genome editing of Caenorhabditis elegans, and a variety of other important topics.

  • Offers a basic understanding and clear picture of genome editing CRISPR-Cas systems in different organisms
  • Explains how to create an animal model for disease diagnosis/research and reprogram CRISPR for removal of virus, bacteria, fungi, protozoan, and many more
  • Discusses the advances, patents, applications, challenges and opportunities in CRISPR-Cas9 systems in basic sciences, biomedicine, virology, bacteriology, molecular biology, and many more
Contributors ix
Preface xi
1 An introduction and use of the CRISPR-Cas systems
1(10)
Vijai Singh
1 Introduction
1(1)
2 History and mechanism of action of CRISPR-Cas systems
2(1)
3 Progress and use of the CRISPR-Cas systems
2(5)
4 Conclusion and future remarks
7(4)
Acknowledgment
8(1)
References
8(3)
2 History, evolution and classification of CRISPR-Cas associated systems
11(66)
Nisheeth Agarwal
Radhika Gupta
1 Introduction
12(1)
2 History of CASSs
13(26)
3 Evolution of type II CRISPR-Cas9 system as a robust gene silencing tool
39(3)
4 Recent advances in understanding the CRISPR-Cas systems
42(19)
5 Classification and evolution of CASSs
61(8)
6 Concluding remarks
69(8)
Acknowledgment
70(1)
References
70(7)
3 CRISPR based bacterial genome editing and removal of pathogens
77(16)
Ravi Jothi
Chandrasekar Karthika
Arumugam Kamaladevi
Lakkakula Satish
Shunmugiah Karutha Pandian
Shanmugaraj Gowrishankar
1 Introduction
78(1)
2 Discovery of CRISPR/Cas9 system
79(1)
3 CRISPR/Cas9: A bacterial adaptive immune mechanism
80(1)
4 Other components in CRISPR/Cas system
80(1)
5 Mechanism of action of gene editing using CRISPR/Cas9 system
81(3)
6 Strategy of genome editing in bacteria
84(2)
7 Gene editing in beneficial bacteria
86(1)
8 Removal of pathogenic bacteria
86(1)
9 Advantages and disadvantages of CRISPR/Cas9 system
87(1)
10 Ethical implication of CRISPR/Cas9
88(1)
11 Conclusion
89(4)
Acknowledgments
89(1)
References
90(3)
4 CRISPR based genome editing and removal of human viruses
93(24)
Yuan-Chuan Chen
1 Introduction
94(3)
2 CRISPR based genome editing of human viruses
97(3)
3 Advantages and challenges of genome editing of human viruses based on CRISPR
100(1)
4 CRISPR based removal of human viruses
101(7)
5 Perspectives and limitations of removal of human viruses based on CRISPR
108(3)
6 Conclusion and future remark
111(6)
References
112(5)
5 CRISPR based development of RNA editing and the diagnostic platform
117(44)
Lakkakula Satish
Gunamalai Lavanya
Thirupathi Kasthuri
Aruchamy Kalaivaani
Sasanala Shamili
Pandiyan Muthuramalingam
Shanmugaraj Gowrishankar
Shunmugiah Karutha Pandian
Vijai Singh
Yaron Sitrit
Ariel Kushmaro
1 Introduction
118(2)
2 Recent updates in CRISPR-Cas9 technology
120(1)
3 Comparison of ZFN, TALEN and CRISPR-Cas9 platforms
121(5)
4 Molecular mechanism of CRISPR-based RNA editing
126(1)
5 CRISPR-Cas-based biosensors/biosensing devices
127(7)
6 Mechanism of CRISPR-based diagnostic tool
134(4)
7 Types of CRISPR-based diagnostic methods
138(14)
8 Conclusions and future directions
152(9)
References
154(7)
6 CRISPR-based pathogenic fungal genome editing for control of infection and disease
161(36)
Takayuki Arazoe
1 Introduction
162(3)
2 Genome editing in plant pathogenic fungi and fungus-like oomycetes
165(13)
3 Genome editing in human and animal pathogenic fungi
178(10)
4 Conclusion and future perspective
188(7)
References
189(6)
Further reading
195(2)
7 Genome engineering in insects for the control of vector borne diseases
197(28)
V. Edwin Hillary
S. Antony Ceasar
1 Introduction
198(2)
2 Vector insects
200(2)
3 Vector control
202(2)
4 Genome engineering
204(4)
5 Application of genome engineering to control vector insects
208(7)
6 Gene drive
215(3)
7 Conclusion and future prospects
218(7)
References
218(7)
Index 225
Dr Vijai Singh is a Professor and Dean (Research & Innovation) at School of Sciences, Indrashil University, Rajpur, Mehsana, Gujarat, India. He was an Associate Professor in the Department of Biosciences, School of Sciences, Indrashil University, Rajpur, Mehsana, Gujarat, India. Prior this this, he was an Assistant Professor in the Department of Biological Sciences and Biotechnology at the Institute of Advanced Research, Gandhinagar, India and also an Assistant Professor in the Department of Biotechnology at the Invertis University, Bareilly, India. Prior to that, he was a Postdoctoral Fellow in the Synthetic Biology Group at the Institute of Systems and Synthetic Biology, Paris, France and School of Energy & Chemical Engineering at the Ulsan National Institute of Science and Technology, Ulsan, South Korea. He received his Ph.D. in Biotechnology from the National Bureau of Fish Genetic Resources, Uttar Pradesh Technical University, Lucknow, India with a research focus on the development of molecular and immunoassays for diagnosis of Aeromonas hydrophila. His research interests are focused on building novel biosynthetic pathways for production of medically and industrially important biomolecules. Additionally, his laboratory is working on CRISPR-Cas9 tools for genome editing. He has more than 11 years of research and teaching experience in synthetic biology, metabolic engineering, bioinformatics, microbiology, and industrial microbiology. He has published 100 articles, 70 chapters, 15 books and 3 patents. He serves as an associate editor, editorial board member, and reviewer of several peer-reviewed journals. He is also a member of the Board of Study and Academic Council of Indrashil University and is the Member Secretary of the Institutional Biosafety Committee (IBSC) at the same University.