This volume contains a compilation of techniques and laboratory protocols directly related to RNA nano technology and its applications in nano biotechnology and nano medicine. The chapters in this book cover a wide range of research methods that can be easily comprehended and carried out in a step-by-step manner by graduate students and postdoctoral fellows from diverse scientific disciplines. Some of the covered topics include various biochemical, biophysical and bioinformatics approaches for analyzing RNA structures and properties; methods to analyze the multi-step assembly process of RNA nano architectures; purification of multifunctional RNA nanoparticles by ultracentrifugation and HPLC; real-time detection of RNA nano constructs in vivo; conjugation of imaging, targeting and therapeutic modules to RNA scaffold; and design and characterization of RNA-protein nanostructures. Written in the highly successful Methods of Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and key tips on troubles troubleshooting and avoiding known pitfalls.
Cutting-edge and practical, RNA Nanotechnology and Therapeutics: Methods and Protocols propels the field toward new heights by engaging researchers working in the diverse fields of physical, engineering, and life sciences.
Overview of Methods in RNA Nanotechnology - Synthesis, Purification and
Characterization of RNA Nanoparticles.- Multiple Approaches for the
Investigation of Bacterial Small Regulatory RNAs Self-Assembly.- Measuring
the Elasticity of Ribonucleotide(s)-Containing DNA Molecules Using AFM.-
Silver Nano clusters for RNA Nano technology: Steps Towards Visualization and
Tracking of RNA Nanoparticle Assemblies.- Purification of RNA Nanoparticles
by Ultracentrifugation.- HPLC Purification of RNA Aptamers up to 59
Nucleotides with Single Nucleotide Resolution.- RNA Nanoparticles with
Thermostable Motifs and Fluorogenic Modules for Real-Time Detection of RNA
Folding and Turnover In Vivo.- Fluorescence Labeling of Short RNA by
Oxidation at the 3-End.- Methods and Assays for Specific Targeting and
Delivery of RNA Nanoparticles to Cancer Metastases.- Functional Assays for
Specific Targeting and Delivery of RNA Nanoparticles to Brain Tumor.-
Aptamer-Mediated Nanoparticle Interactions: From Oligonucleotide-Protein
Complexes to SELEX Screens.- Methods for Assembling B-Cell Lymphoma Specific
and Internalizing Aptamer-siRNA Nanoparticles Via the Sticky Bridge.- A High
Throughput Screening Assay for the Functional Delivery of Splice-Switching
Oligonucleotides in Human Melanoma Cells.- Design, Assembly, and Evaluation
of RNA-Protein Nanostructures.- Mapping RNA Interactions to Proteins in
Virions Using CLIP-Seq.- Mapping Protein-RNA Interactions by RCAP,
RNA-Crosslinking and Peptide Fingerprinting.
Peixuan Guo, Ph. D. William Farish Endowed Chair in Nano biotechnology Professor of School of Pharmacy Director of Nano biotechnology Center University of Kentucky Director of NCI Cancer Nanotechnology Platform Partnership Program: RNA Nanotechnology for Cancer Therapy Mailing address College of Pharmacy University of Kentucky 565 Biopharm Complex 789 S. Limestone Street Lexington, KY 40536 Phone: (Office) (859)218-0128 (Cell Phone): (513)728-1411 peixuan.guo@uky.edu (future)
