E-grāmata: Serum/Plasma Proteomics: Methods and Protocols

Protocols for the Isolation of Platelets for Research and Contrast to
Production of Platelet Concentrates for Transfusion.- Collection of plasma
samples in areas with limited healthcare access.- Blood Collection Processing
& Handling for Plasma and Serum Proteomics.- Preparation of cryoprecipitate
and cryo-depleted plasma for proteomic research analysis.- High throughput
proteome profiling of plasma and native plasma complexes using native
chromatography.- High-throughput and in-depth proteomic profiling of 5 µL
plasma and serum using TMTpro 16plex.- In depth data-independent acquisition
proteomics of human plasma identifies low abundant proteins.- In-depth Blood
Proteome Profiling by Extensive Fractionation and multiplexed quantitative
mass spectrometry.- Early cancer biomarker discovery using DIA-MS proteomic
analysis of EVs from peripheral blood.- Strategies to enrich, identify and
characterize glycoproteome in blood plasma using Liquid Chromatography High
Resolution Mass Spectrometry.- Proteome analysis of whole blood collected by
volumetric absorptive microsampling.- Proteomic applications and
considerations: from research to patient care.- Immunoaffinity mass
spectrometry diagnostic tests for multi-biomarker assays.- Secretome profile
of leukocyte-platelet rich fibrin (L-PRF) membranes.- Quantification of
proteins in blood by absorptive microtiter plate-based affinity purification
coupled to liquid chromatography-mass spectrometry.- Glycomics-assisted
Glycoproteomics Enables Deep and Unbiased N-Glycoproteome Profiling of
Complex Biological Specimens. -The Small Protein Enrichment Assay (SPEA) for
analysis of low abundance peptide hormones in plasma.- In-depth proteomic
analysis of blood circulating small extracellular vesicles.- Protocol for
Plasma Extracellular Vesicle and Particle Isolation and Mass
Spectrometry-based Proteomic Identification.- Analysis of
ExtracellularVesicle and Contaminant Markers in Blood Derivatives Using
Multiple Reaction Monitoring.- Generation of red blood cell nanovesicles as a
delivery tool.- Progress in targeted mass spectrometry (parallel
accumulation-serial fragmentation) and its application in plasma/serum
proteomics.- Offline peptide fractionation and parallel reaction monitoring
MS for the quantitation of low-abundance plasma proteins.- Profiling Serum
Intact N-Glycopeptides Using Data-Independent Acquisition Mass
Spectrometry.- Semi-automated lectin magnetic bead array (LeMBA) for
translational serum glycoprotein biomarker discovery and
validation.- Accessing antibody-reactivities in serum or plasma to
(auto-)antigens using multiplexed bead-based protein immunoassays.- Absolute
quantitative targeted proteomics assays for plasma proteins.- Rapid and
quantitative enrichment of peptides from plasma for mass spectrometric
analysis.- Comprehensive targeted lipidomic profilingfor research and
clinical applications.- Multiplexed bead-based peptide immunoassays for the
detection of antibody reactivities.- Array-based multiplex and
high-throughput serology assays.- Bioinformatic tools and knowledgebases to
assist generating targeted assays for plasma proteomics.- Use of Longitudinal
Serum Analysis and Machine Learning to Develop a Classifier for Cancer Early
Detection.