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Serum/Plasma Proteomics: Methods and Protocols Third Edition 2023 [Mīkstie vāki]

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  • Formāts: Paperback / softback, 596 pages, height x width: 254x178 mm, weight: 1156 g, 50 Illustrations, color; 10 Illustrations, black and white; XVIII, 596 p. 60 illus., 50 illus. in color., 1 Paperback / softback
  • Sērija : Methods in Molecular Biology 2628
  • Izdošanas datums: 14-Feb-2024
  • Izdevniecība: Springer-Verlag New York Inc.
  • ISBN-10: 1071629808
  • ISBN-13: 9781071629802
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Serum/Plasma Proteomics: Methods and Protocols Third Edition 2023Palielināt
  • Formāts: Paperback / softback, 596 pages, height x width: 254x178 mm, weight: 1156 g, 50 Illustrations, color; 10 Illustrations, black and white; XVIII, 596 p. 60 illus., 50 illus. in color., 1 Paperback / softback
  • Sērija : Methods in Molecular Biology 2628
  • Izdošanas datums: 14-Feb-2024
  • Izdevniecība: Springer-Verlag New York Inc.
  • ISBN-10: 1071629808
  • ISBN-13: 9781071629802
Citas grāmatas par šo tēmu:
Permanent link: https://www.kriso.lv/db/9781071629802.html
Keywords:
This third volume provides comprehensive protocols on pre-analytical, analytical, plasma, and serum proteomics. New and updated chapters are divided into nine sections, detailing blood processing and handling strategies, discovery- and targeted-based mass spectrometry, including workflows to aid in discovery and targeted data analysis, in addition to software and bioinformatics for the plasma proteome. This edition further integrates emerging areas in the development of technologies for plasma proteomics and assay platforms in biomarker discovery and translational proteomics, enrichment and detection strategies to understand the plasma proteome, and peptide, lipid and metabolite targeted assays. We also detail the emerging analysis of extracellular vesicles isolated from plasma.





 





Written in the format of the highly successful Methods in Molecular Biology series, each of the 33 chapters includes an introduction to the topic, lists necessary materials and methods, includes hints and tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.





 





Authoritative and cutting-edge, Serum/Plasma Proteomics: Methods and Protocols, Third Edition aims to be comprehensive guide for researchers.





 
Protocols for the Isolation of Platelets for Research and Contrast to
Production of Platelet Concentrates for Transfusion.- Collection of plasma
samples in areas with limited healthcare access.- Blood Collection Processing
& Handling for Plasma and Serum Proteomics.- Preparation of cryoprecipitate
and cryo-depleted plasma for proteomic research analysis.- High throughput
proteome profiling of plasma and native plasma complexes using native
chromatography.- High-throughput and in-depth proteomic profiling of 5 µL
plasma and serum using TMTpro 16plex.- In depth data-independent acquisition
proteomics of human plasma identifies low abundant proteins.- In-depth Blood
Proteome Profiling by Extensive Fractionation and multiplexed quantitative
mass spectrometry.- Early cancer biomarker discovery using DIA-MS proteomic
analysis of EVs from peripheral blood.- Strategies to enrich, identify and
characterize glycoproteome in blood plasma using Liquid Chromatography High
Resolution Mass Spectrometry.- Proteome analysis of whole blood collected by
volumetric absorptive microsampling.- Proteomic applications and
considerations: from research to patient care.- Immunoaffinity mass
spectrometry diagnostic tests for multi-biomarker assays.- Secretome profile
of leukocyte-platelet rich fibrin (L-PRF) membranes.- Quantification of
proteins in blood by absorptive microtiter plate-based affinity purification
coupled to liquid chromatography-mass spectrometry.- Glycomics-assisted
Glycoproteomics Enables Deep and Unbiased N-Glycoproteome Profiling of
Complex Biological Specimens. -The Small Protein Enrichment Assay (SPEA) for
analysis of low abundance peptide hormones in plasma.- In-depth proteomic
analysis of blood circulating small extracellular vesicles.- Protocol for
Plasma Extracellular Vesicle and Particle Isolation and Mass
Spectrometry-based Proteomic Identification.- Analysis of
ExtracellularVesicle and Contaminant Markers in Blood Derivatives Using
Multiple Reaction Monitoring.- Generation of red blood cell nanovesicles as a
delivery tool.- Progress in targeted mass spectrometry (parallel
accumulation-serial fragmentation) and its application in plasma/serum
proteomics.- Offline peptide fractionation and parallel reaction monitoring
MS for the quantitation of low-abundance plasma proteins.- Profiling Serum
Intact N-Glycopeptides Using Data-Independent Acquisition Mass
Spectrometry.- Semi-automated lectin magnetic bead array (LeMBA) for
translational serum glycoprotein biomarker discovery and
validation.- Accessing antibody-reactivities in serum or plasma to
(auto-)antigens using multiplexed bead-based protein immunoassays.- Absolute
quantitative targeted proteomics assays for plasma proteins.- Rapid and
quantitative enrichment of peptides from plasma for mass spectrometric
analysis.- Comprehensive targeted lipidomic profilingfor research and
clinical applications.- Multiplexed bead-based peptide immunoassays for the
detection of antibody reactivities.- Array-based multiplex and
high-throughput serology assays.- Bioinformatic tools and knowledgebases to
assist generating targeted assays for plasma proteomics.- Use of Longitudinal
Serum Analysis and Machine Learning to Develop a Classifier for Cancer Early
Detection.