This volume discusses the latest techniques used in the diverse fields of single cell omics and covers topics such as quantifying the single cell transcriptome; isolation of cells in nanoliter volumes for single cell proteomics measurements by nano-LC-MS/MS; and single cell protein characterization by immunoblotting. A wide range of methodologies are highlighted, ranging from high-yield chemical amplification to mass spectrometry and nanotechnology for the analysis of the chemical constituents of cells. In the Neuromethods series style, chapters include both in-depth overviews, as well as detailed protocols that provide the key advice from specialists you need to get successful results in your laboratory.
Cutting-edge and comprehensive, Single Cell Omics of Neuronal Cells is a valuable resource for experienced and novice researchers interested in learning more about this field and its future developments.
Singe Cell aRNA-Seq for Transcriptomics and Cellular
Phenotyping.- Single Cell Genomic and Epigenomic DNA Analyses.- Single-Cell
Proteome Profiling of Neuronal Cells.- Joint Analysis of Transcriptome and
Proteome Measurements in Single Cells with TotalVI.- Single-Cell Mass
Spectrometry of Metabolites and Proteins for Systems and Functional
Biology.- High-Throughout Image-Guided Microprobe Mass Spectrometric Analysis
of Single Cells.- Single-Cell Resolution Immunoblotting.- Quantifying Enzyme
Activity and Gene Expression within Single-Cells using a Multiplexed
Capillary Electrophoresis Platform.- Quantitative Chemical Imaging at the
Cell Level-SIMS, Fluorescence, and Correlative Techniques.- Electrophysiology
and Single Cells.- Genetically Encoded Biosensors Reveal Spatiotemporal
Dynamics and Cellular Heterogeneity of Neuronal Cells.
