| Preface |
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| Contributors |
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PART I INTRODUCTION TO SINGLE-DOMAIN ANTIBODIES |
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1 Nanobodies: From Serendipitous Discovery of Heavy Chain-Only Antibodies in Camelids to a Wide Range of Useful Applications |
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3 | (16) |
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2 Overview, Generation, and Significance of Variable New Antigen Receptors (VNARs) as a Platform for Drug and Diagnostic Development |
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19 | (18) |
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PART II ISOLATION OF SINGLE-DOMAIN ANTIBODIES |
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3 Llama DNA Immunization and Isolation of Functional Single-Domain Antibody Binders |
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37 | (34) |
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4 Preparation of Immune and Synthetic VNAR Libraries as Sources of High-Affinity Binders |
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71 | (24) |
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Jahaziel Gasperin-Bulbarela |
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5 Isolation of Single-Domain Antibodies to Transmembrane Proteins Using Magnetized Yeast Cell Targets |
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95 | (26) |
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6 A Transgenic Heavy Chain IgG Mouse Platform as a Source of High Affinity Fully Human Single-Domain Antibodies for Therapeutic Applications |
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121 | (24) |
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PART III EXPRESSION OF SINGLE-DOMAIN ANTIBODIES |
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7 Cytoplasmic Production of Nanobodies and Nanobody-Based Reagents by Co-Expression of Sulfhydryl Oxidase and DsbC Isomerase |
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145 | (14) |
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8 Small-Scale Secretory VHH Expression in Saccharomyces cerevisiae |
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159 | (22) |
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Marga van Hagen-van Setten |
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9 Production of Single-Domain Antibodies in Pichia pastoris |
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181 | (24) |
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10 Production of Designer VHH-Based Antibodies in Plants |
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205 | (28) |
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PART IV SINGLE-DOMAIN ANTIBODY ENGINEERING AND CHARACTERIZATION |
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11 Assessing the Aggregation Propensity of Single-Domain Antibodies upon Heat-Denaturation Employing the ATm Shift |
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233 | (12) |
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12 Facile Affinity Maturation of Single-Domain Antibodies Using Next-Generation DNA Sequencing |
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245 | (24) |
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13 Engineering pH-Sensitive Single-Domain Antibodies |
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269 | (30) |
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14 Humanization of Camelid Single-Domain Antibodies |
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299 | (14) |
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15 Creation of Multimeric Single-Domain Antibodies Using Bacterial Superglues |
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313 | (14) |
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16 Introducing Cysteines into Nanobodies for Site-Specific Labeling |
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327 | (18) |
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17 Affinity-Guided Site-Selective Labeling of Nanobodies with Aldehyde Handles |
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345 | (12) |
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18 Cytoplasmic Expression of Nanobodies with Formylglycine Generating Enzyme Tag and Conversion to a Bio-Orthogonal Aldehyde Group |
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357 | (16) |
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19 Site-Specific Fluorescent Labeling, Single-Step Immunocytochemistry, and Delivery of Nanobodies into Living Cells |
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373 | (22) |
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20 Design and Validation of Site-Specifically Labeled Single-Domain Antibody-Based Tracers for in Vivo Fluorescence Imaging and Image-Guided Surgery |
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395 | (14) |
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21 Design and Preparation of Photobodies: Light-Activated Single-Domain Antibody Fragments |
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409 | (18) |
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PART V APPLICATIONS OF SINGLE-DOMAIN ANTIBODIES |
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22 Visualizing Filoviral Nucleoproteins Using Nanobodies Fused to the Ascorbate Peroxidase Derivatives APEX2 and dEAPX |
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427 | (24) |
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23 Development of Glypican-2 Targeting Single-Domain Antibody CART Cells for Neuroblastoma |
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451 | (18) |
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24 Single-Domain Antibodies for Intracellular Toxin Neutralization |
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469 | (20) |
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25 Generation of Single-Domain Antibody-Based Recombinant Immunotoxins |
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489 | (24) |
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26 X-ray Crystal Structure Analysis of VHH-Protein Antigen Complexes |
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513 | (18) |
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27 Functionalization of Magnetic Beads with Biotinylated Nanobodies for MALDI-TOF/MS-Based Quantitation of Small Analytes |
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531 | (16) |
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Gualberto Gonzalez-Sapienza |
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28 Nanobody-Based Assays for the Detection of Environmental and Agricultural Contaminants |
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547 | (8) |
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29 Peptide-Tag Specific Nanobodies for Studying Proteins in Live Cells |
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555 | (26) |
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30 Nanobody-Based GFP Traps to Study Protein Localization and Function in Developmental Biology |
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581 | (14) |
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31 Cytogenetic Activation of Intracellular Nanobodies |
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| Correction to: Cytogenetic Activation of Intracellular Nanobodies |
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1 | (606) |
| Index |
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607 | |
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