Recent advances in understanding the biological role of singlet oxygen in the pathways of cellular responses to ultraviolet-A radiation: its key position in photodynamical effects, and its generation by photochemical (dark) reactions, e.g. by cells of the immune system such as eosinophils and macrophages, are the focus of this volume. The new methods and techniques responsible for the rapid progress in this area are presented.
The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today--truly an essential publication for researchers in all fields of life sciences.
Recenzijas
Praise for the Series "The Methods in Enzymology series represents the gold-standard." --NEUROSCIENCE "Incomparably useful." --ANALYTICAL BIOCHEMISTRY "It is a true 'methods' series, including almost every detail from basic theory to sources of equipment and reagents, with timely documentation provided on each page." --BIO/TECHNOLOGY "The series has been following the growing, changing and creation of new areas of science. It should be on the shelves of all libraries in the world as a whole collection." --CHEMISTRY IN INDUSTRY "The appearance of another volume in that excellent series, Methods in Enzymology, is always a cause for appreciation for those who wish to successfully carry out a particular technique or prepare an enzyme or metabolic intermediate without the tiresome prospect of searching through unfamiliar literature and perhaps selecting an unproven method which is not easily reproduced." --AMERICAN SOCIETY OF MICROBIOLOGY NEWS "If we had some way to find the work most often consulted in the laboratory, it could well be Colowick and Kaplan's multi-volume series Methods in Enzymology...a great work." --ENZYMOLOGIA "A series that has established itself as a definitive reference for biochemists." --JOURNAL OF CHROMATOGRAPHY
Contributors to Volume 319 xi Preface xvii Volumes in Series xix Section I. Singlet Oxygen Generation and Detection: Chemical Systems Naphthalene Endoperoxides as Generators of Singlet Oxygen in Biological Media 3(17) Christel Pierlot Jean-Marie Aubry Karlis Briviba Helmut Sies Paolo Di Mascio Photosensitized Production of Singlet Oxygen 20(9) Irene E. Kochevar Robert W. Redmond Singlet Oxygen from Irradiated Titanium Dioxide and Zinc Oxide 29(8) Yorihiro Yamamoto Noritaka Imai Ryuichi Mashima Ryusei Konaka Masayasu Inoue Walter C. Dunlap Time-Resolved Singlet Oxygen Detection 37(13) Santi Nonell Silvia E. Braslavsky Measurement of Photogenerated Singlet Oxygen in Aqueous Media 50(9) Veronique Nardello Jean-Marie Aubry Generation and Detection: Biological Systems Assay for Singlet-Oxygen Generation by Peroxi-dases Using 1270-nm Chemiluminescence 59(8) Jeffrey R. Kanofsky Formation of Electronically Excited States during the Oxidation of Arachidonic Acid by Prostaglandin Endoperoxide Synthase 67(10) Enrique Cadenas Helmut Sies Singlet Oxygen Detection with Sterically Hindered Amine Derivatives in Plants under Light Stress 77(8) Eva Hideg Imre Vass Tamas Kalai Kalman Hideg Cholesterol as a Singlet Oxygen Detector in Biological Systems 85(16) Albert W. Girotti Witold Korytowski Singlet Oxygen Scavenging in Phospholipid Membranes 101(9) Kenji Fukuzawa Catalase Modification as a Marker for Singlet Oxygen 110(9) Fernando Lledias Wilhelm Hansberg Biological Effects: Role in Signaling Nuclear Factor-kB Activation by Singlet Oxygen Produced during Photosensitization 119(11) Jean-Yves Matroule Jacques Piette Mitogen-Activated Protein Kinase Activation by Singlet Oxygen and Ultraviolet A 130(13) Lars-Oliver Klotz Karlis Briviba Helmut Sies Toxic Effects Singlet Oxygen DNA Damage Products: Formation and Measurement 143(10) Jean Cadet Thierry Douki Jean-Pierre Pouget Jean-Luc Ravanet Ultraviolet A- and Singlet Oxygen-Induced Mutation Spectra 153(12) Anne Stary Alain Sarasin Damage to DNA by Long-Range Charge Transport 165(23) Megan E. Nunez Scott R. Rajski Jacqueline K. Barton Cholesterol Photodynamic Oxidation by Ultraviolet Irradiation and Cholesterol Ozonization by Ozone Exposure 188(9) Kyoichi Osada Alex Sevanian Bactericidal and Virucidal Activities of Singlet Oxygen Generated by Thermolysis of Naphthalene Endoperoxides 197(10) Corinne Pellieux Anny Dewilde Christel Pierlot Jean-Marie Aubry Inactivation of Viruses in Human Plasma 207(9) Harald Mohr 3-(4-Methyl-1-naphthyl)propionic Acid, 1,4-Endoperoxide for Dioxygenation of Squalene and for Bacterial Killing 216(6) Minoru Nakano Yasuhiro Kambayashi Hidetaka Tatsuzawa Protection: Singlet Oxygen Quenchers Biological Singlet Oxygen Quenchers Assessed by Monomol Light Emission 222(4) Karlis Briviba Helmut Sies Synthetic Singlet Oxygen Quenchers 226(19) Stefan Beutner Britta Bloedorn Thomas Hoffmann Hans-Dieter Martin Section II. Ultraviolet A Dosimetry Dosimetry of Ultraviolet A Radiation 245(10) Brian L. Diffey Biological Responses: Signaling Radiation-Induced Signal Transduction 255(18) Axel Knebel Frank D. Bohmer Peter Herrlich Signaling Pathways Leading to Nuclear Factor-κB Activation 273(7) Nanxin Li Michael Karin Gene Regulation by Ultraviolet A Radiation and Singlet Oxygen 280(10) Susanne Grether-Beck Jean Krutmann Role for Singlet Oxygen in Biological Effects of Ultraviolet A Radiation 290(6) Rex M. Tyrrell Ultraviolet A-1 Irradiation as a Tool to Study the Pathogenesis of Atopic Dermatitis 296(6) Jean Krutmann Ultraviolet A Radiation-Induced Apoptosis 302(7) Akimichi Morita Jean Krutmann Singlet Oxygen-Triggered Immediate Preprogrammed Apoptosis 309(22) Dianne E. Godar Determination of DNA Damage, Peroxide Generation, Mitochondrial Membrane Potential, and Caspase-3 Activity during Ultraviolet A-Induced Apoptosis 331(11) Saeko Tada-Oikawa Shinji Oikawa Shosuke Kawanishi Mechanism of Photodynamic Therapy-Induced Cell Death 342(17) Nihal Ahmad Hasan Mukhtar Biological Responses: Photocarcinogenesis, Photoaging, and Photoallergic Reactions Photocarcinogenesis: UVA vs UVB 359(7) Frank R. de Gruijl Photoaging-Associated Large-Scale Deletions of Mitochondrial DNA 366(10) Mark Berneburg Jean Krutmann Role of Activated Oxygen Species in Photodynamic Therapy 376(25) Wesley M. Sharman Cynthia M. Allen Johan E. van Lier Gas Chromatography-Mass Spectrometry Analysis of DNA: Optimization of Protocols for Isolation and Analysis of DNA from Human Blood 401(16) Almas Rehman Andrew Jenner Barry Halliwell Oxidative Damage Markers Sequence Specificity of Ultraviolet A-Induced DNA Damage in the Presence of Photosensitizer 417(11) Kimiko Ito Shosuke Kawanishi Protein Oxidative Damage 428(8) Emily Shacter DNA Damage Induced by Ultraviolet and Visible Light and Its Wavelength Dependence 436(9) Christopher Kielbassa Bernd Epe Photoprotection of Skin against Ultraviolet A Damage 445(20) Hans Schaefer Alain Chardon Dominique Moyal Protection Topically Applied Antioxidants in Skin Protection 465(14) Franz Stab Rainer Wolber Thomas Blatt Reza Keyhani Gerhard Sauermann Erythropoietic Protoporphyria: Treatment with Antioxidants and Potential Cure with Gene Therapy 479(6) Micheline M. Mathews-Roth Porphyrias: Photosensitivity and Phototherapy 485(9) Maureen B. Poh-Fitzpatrick Carotenoids in Human Skin: Noninvasive Measurement and Identification of Dermal Carotenoids and Carotenol Esters 494(11) Wilhelm Stahl Ulrike Heinrich Holger Jungmann Hagen Tronnier Helmut Sies Section III. Ozone Reactive Absorption of Ozone: An Assay for Reaction Rates of Ozone with Sulfhydryl Compounds and with Other Biological Molecules 505(7) Jeffrey R. Kanofsky Paul D. Sima Assay for Singlet Oxygen Generation by Plant Leaves Exposed to Ozone 512(8) Jeffery R. Kanofsky Paul D. Sima Ozone Effects on Plant Defense 520(16) Christian Langebartels Dieter Ernst Jaakko Kangasjarvi Heinrich Sandermann, Jr. High-Pressure Liquid Chromatography Analysis of Ozone-Induced Depletion of Hydrophilic and Lipophilic Antioxidants in Murine Skin 536(10) Stefan U. Weber Sumana Jothi Jens J. Thiele Reactions of Vitamin E with Ozone 546(5) Daniel C. Liebler Induction of Nuclear Factor-κB by Exposure to Ozone and Inhibition by Glucocorticoids 551(11) Kian Fan Chung Ian M. Adcock Detection of 4-Hydroxy-2-nonenol Adducts Following Lipid Peroxidation from Ozone Exposure 562(8) Luke I. Szweda Pamela A. Szweda Andrij Holian Synthesis of Inflammatory Signal Transduction Species Formed during Ozonation and/or Peroxidation of Tissue Lipids 570(15) Giuseppe L. Squadrito Maria G. Salgo Frank R. Fronczek William A. Pryor Section IV. General Methods Assay for Redox-Sensitive Transcription Factor 585(18) Madan M. Chaturvedi Asok Mukhopadhyay Bharat B. Aggarwal Fluorescent Fatty Acid to Monitor Reactive Oxygen in Single Cells 603(9) Eward H. W. Pap G. P. C. Drummen J. A. Post P. J. Rijken K. W. A. Wirtz Noninvasive Techniques for Measuring Oxidation Products on the Surface of Human Skin 612(11) Daniel Maes Tom Mommone MaryAnn McKeever Ed Pelle Christina Fthenakis Lieve Declercq Paolo U. Giacomoni Ken Marenus Author Index 623(38) Subject Index 661
Lester Packer received a PhD in Microbiology and Biochemistry in 1956 from Yale University. In 1961, he joined the University of California at Berkeley serving as Professor of Cell and Molecular Biology until 2000, and then was appointed Adjunct Professor, Pharmacology and Pharmaceutical Sciences, School of Pharmacy at the University of Southern California. Dr Packer received numerous distinctions including three honorary doctoral degrees, several distinguished Professor appointments. He was awarded Chevalier de lOrdre National du Merite (Knight of the French National Order of Merit) and later promoted to the rank of Officier. He served as President of the Society for Free Radical Research International (SFRRI), founder and Honorary President of the Oxygen Club of California. He has edited numerous books and published research; some of the most cited articles have become classics in the field of free radical biology: Dr Packer is a member of many professional societies and editorial boards. His research elucidated - the Antioxidant Network concept. Exogenous lipoic acid was discovered to be one of the most potent natural antioxidants and placed as the ultimate reductant or in the pecking order of the Antioxidant Network regenerating vitamins C and E and stimulating glutathione synthesis, thereby improving the overall cellular antioxidant defense. The Antioxidant Network is a concept addressing the cells redox status. He established a world-wide network of research programs by supporting and co-organizing conferences on free radical research and redox biology in Asia, Europe, and America. Helmut Sies, MD, PhD (hon), studied medicine at the universities of Tübingen, Munich, and Paris. He was the professor and chair of the Institute for Biochemistry and Molecular Biology I at Heinrich-Heine-University Düsseldorf, Germany, where he is now professor emeritus. He is a member of the German National Academy of Sciences Leopoldina and was the president of the North Rhine-Westphalian Academy of Sciences and Arts. He was named Redox Pioneer; was the president of the Society for Free Radical Research International (SFRRI). Helmut Sies introduced the concept of Oxidative Stress in 1985, and was the first to reveal hydrogen peroxide as a normal constituent of aerobic cell metabolism. His research interests comprise redox biology, oxidants, antioxidants, micronutrients.
