E-grāmata: Skin Stem Cells: Methods and Protocols

Isolation, Characterization, and Utilization of Human Skin Basal and Supra-Basal Epidermal Stem Cells.- Isolation of Human Progenitor Epidermal Cells on Collagen Type IV and Analysis of Their Markers with Flow Cytometry and PCR Methods.- Approaches to Study Wound-Induced Hair Neogenesis (WIHN).- Characterization of the Newborn Epidermis and Adult Hair Follicles Using Whole-Mount Immunofluorescent Staining of Mouse Dorsal Skin.- Preparation and Use of shRNA for Knocking Down Specific Genes.- Efficient Generation of CRISPR/Cas9-Mediated Knockout Human Primary Keratinocytes by Electroporation.- Application of CRISPR/Cas9 Pooled Screening for a Non-Immediate Readout Model.- Contribution of Artificial Intelligence to the Identification of Protein-Protein Interactions: A Case Study on PAR-3 and Its Partner Adapter Molecule Crk.- Site-Directed Mutagenesis to Mutate Multiple Residues in a Single Reaction.- Analysis of Proliferation, Apoptosis, and Motility in Mouse Embryonic Melanocytic Precursor Cells.- Protocol for Testing Human Melanoma Exosomes in Shifting Healthy Phenotype of Human Dermal Cells.- Incorporating NK Cells in a Three-Dimensional Organotypic Culture System for Human Skin Stem Cells: Modeling Skin Diseases and Immune Cell Interplay.- Standardized GMP-Compliant Scalable 3D-Bioprocessing of Epidermal Stem Cells for Diabetic Foot Ulcers.- Differentiation and Validation of Human Induced Pluripotent Stem Cells into a Keratinocyte, as Therapeutic Option in Vitiligo.- GMP-Based Isolation of Full-Term Human Placenta-Derived NK Cells for CAR-NK Cell Therapy in Malignant Melanoma.- Isolation and Phenotypic Characterization of Tumor-Infiltrating NK Cells in Skin Carcinoma.- Preparation and Validation of Zebrafish Psoriasis Model to Investigate the Therapeutic Effects of Stem Cells.- Flow Cytometric and Immunohistochemical Follow-Up of Spermatogonial Lineage Commitment.- Development of CAR-NK Cells Targeting cSCC-Specific Antigens for Precision Immunotherapy.